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Simultaneous measurement of arginine vasopressin and oxytocin in plasma and neurohypophysis by radioimmunoassay

Abstract

Arginine vasopressin (AVP) and oxytocin (OXT) were measured simultaneously in the same sample by specific and sensitive radioimmunoassays (RIAs). The antibodies used did not cross-react to a variety of analogs and related peptides. The extraction procedure using Vycor glass powder resulted in mean recoveries of 84.4% (AVP) and 64.6% (OXT). In both assays, the sensitivity was 1 to 2 pg/ml plasma. A preincubation procedure that depresses plasma levels of both AVP and OXT selectively, provided specific blank values for a given plasma sample. To confirm the validity of the RIAs, dehydration experiments were performed. In rats, the basal levels of plasma AVP and OXT (means: 2,63 pg/ml and 6.80 pg/ml, respectively) are increased significantly after 24 h, 48 h and 72 h of water deprivation. Relationships are presented between both neurohormones in the plasma and neurohypophyses of control and dehydrated animals. As shown in cows, a significant correlation exists between plasma AVP and plasma osmolality but not between plasma OXT and osmolality or plasma AVP and OXT. Basal levels as well as physiological changes in plasma and neurohypophyseal AVP and OXT can be measured by the RIAs described.
Authors:
Landgraf, R [1] 
  1. Deutsche Hochschule fuer Koerperkultur, Leipzig (German Democratic Republic). Forschungsinstitut Koerperkultur und Sport
Publication Date:
Dec 01, 1981
Product Type:
Journal Article
Reference Number:
AIX-13-680202; EDB-82-115900
Resource Relation:
Journal Name: Endokrinologie; (German Democratic Republic); Journal Volume: 78:2-3
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; OXYTOCIN; RADIOIMMUNOASSAY; VASOPRESSIN; ACCURACY; BLOOD PLASMA; COWS; DEHYDRATION; IODINE 125; PITUITARY GLAND; RADIOPHARMACEUTICALS; RATS; SENSITIVITY; SPECIFICITY; TIME DEPENDENCE; ANIMALS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BODY; BODY FLUIDS; CATTLE; DAYS LIVING RADIOISOTOPES; DOMESTIC ANIMALS; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; ENDOCRINE GLANDS; GLANDS; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LABELLED COMPOUNDS; MAMMALS; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; ORGANS; PEPTIDE HORMONES; PITUITARY HORMONES; RADIOASSAY; RADIOISOTOPES; RODENTS; RUMINANTS; TRACER TECHNIQUES; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5428192
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: ENDKA
Submitting Site:
HEDB
Size:
Pages: 101-204
Announcement Date:
Jun 01, 1982

Citation Formats

Landgraf, R. Simultaneous measurement of arginine vasopressin and oxytocin in plasma and neurohypophysis by radioimmunoassay. Germany: N. p., 1981. Web.
Landgraf, R. Simultaneous measurement of arginine vasopressin and oxytocin in plasma and neurohypophysis by radioimmunoassay. Germany.
Landgraf, R. 1981. "Simultaneous measurement of arginine vasopressin and oxytocin in plasma and neurohypophysis by radioimmunoassay." Germany.
@misc{etde_5428192,
title = {Simultaneous measurement of arginine vasopressin and oxytocin in plasma and neurohypophysis by radioimmunoassay}
author = {Landgraf, R}
abstractNote = {Arginine vasopressin (AVP) and oxytocin (OXT) were measured simultaneously in the same sample by specific and sensitive radioimmunoassays (RIAs). The antibodies used did not cross-react to a variety of analogs and related peptides. The extraction procedure using Vycor glass powder resulted in mean recoveries of 84.4% (AVP) and 64.6% (OXT). In both assays, the sensitivity was 1 to 2 pg/ml plasma. A preincubation procedure that depresses plasma levels of both AVP and OXT selectively, provided specific blank values for a given plasma sample. To confirm the validity of the RIAs, dehydration experiments were performed. In rats, the basal levels of plasma AVP and OXT (means: 2,63 pg/ml and 6.80 pg/ml, respectively) are increased significantly after 24 h, 48 h and 72 h of water deprivation. Relationships are presented between both neurohormones in the plasma and neurohypophyses of control and dehydrated animals. As shown in cows, a significant correlation exists between plasma AVP and plasma osmolality but not between plasma OXT and osmolality or plasma AVP and OXT. Basal levels as well as physiological changes in plasma and neurohypophyseal AVP and OXT can be measured by the RIAs described.}
journal = []
volume = {78:2-3}
journal type = {AC}
place = {Germany}
year = {1981}
month = {Dec}
}