You need JavaScript to view this

Proton microprobe analysis of pancreatic. beta. cells

Abstract

Freeze-dried pancreas sections from obese hyperglycemic mice were subjected to proton bombardment and the elemental contents in the ..beta.. cells and the exocrine part were obtained from the characteristic X-rays emitted. Quantitative data were provided for 18 different elements. The mole ratio between K and Na exceeded 10, implying that neither the sample preparation nor the irradiation had induced significant diffuse changes. With the demonstration of this high K/Na ratio it seems likely that also the ..beta.. cells are equipped with an efficient Na/sup +//K/sup +/ pump. The ..beta.. cells contained about 70 mmoles Cl per litre cell water. Observed amounts of Ca and Mg were equivalent to those previously recorded by electrothermal atomic absorption spectroscopy. The significant role of Zn for the storage of insulin was emphasized by the demonstration of 3 times as much of this element in the ..beta.. cells as compared with the exocrine pancreas. In addition, the sensitivity of the proton microprobe enabled measurements of various trace elements such as Rb, Cr, Cu, Al and Pb not previously demonstrated in the pancreatic ..beta.. cells.
Authors:
Lindh, U; [1]  Juntti-Berggren, L; Berggren, P O; Hellman, B [2] 
  1. Uppsala Univ. (Sweden). Gustaf Werner Inst.
  2. Uppsala Univ. (Sweden)
Publication Date:
Jan 01, 1985
Product Type:
Conference
Report Number:
CONF-8404254-
Reference Number:
AIX-16-049229; EDB-85-133177
Resource Relation:
Journal Name: Biomed. Biochim. Acta; (Germany, Federal Republic of); Journal Volume: 44:1; Conference: 4. international workshop on isolated pancreatic islets and diabetes, Trassenheide, Germany D.R., 16 Apr 1984
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; ALUMINIUM; PROTON MICROPROBE ANALYSIS; X-RAY SPECTROSCOPY; CALCIUM; CHROMIUM; COPPER; LEAD; MAGNESIUM; PANCREAS; MULTI-ELEMENT ANALYSIS; POTASSIUM; RUBIDIUM; SODIUM; ZINC; CATIONS; INSULIN; MICE; SENSITIVITY; SOMATIC CELLS; TRACE AMOUNTS; ALKALI METALS; ALKALINE EARTH METALS; ANIMAL CELLS; ANIMALS; BODY; CHARGED PARTICLES; CHEMICAL ANALYSIS; DIGESTIVE SYSTEM; ELEMENTS; ENDOCRINE GLANDS; GLANDS; HORMONES; IONS; MAMMALS; METALS; MICROANALYSIS; NONDESTRUCTIVE ANALYSIS; ORGANS; PEPTIDE HORMONES; RODENTS; SPECTROSCOPY; TRANSITION ELEMENTS; VERTEBRATES; 400103* - Radiometric & Radiochemical Procedures- (-1987); 550300 - Cytology
OSTI ID:
5424805
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BBIAD
Submitting Site:
HEDB
Size:
Pages: 55-62
Announcement Date:

Citation Formats

Lindh, U, Juntti-Berggren, L, Berggren, P O, and Hellman, B. Proton microprobe analysis of pancreatic. beta. cells. Germany: N. p., 1985. Web.
Lindh, U, Juntti-Berggren, L, Berggren, P O, & Hellman, B. Proton microprobe analysis of pancreatic. beta. cells. Germany.
Lindh, U, Juntti-Berggren, L, Berggren, P O, and Hellman, B. 1985. "Proton microprobe analysis of pancreatic. beta. cells." Germany.
@misc{etde_5424805,
title = {Proton microprobe analysis of pancreatic. beta. cells}
author = {Lindh, U, Juntti-Berggren, L, Berggren, P O, and Hellman, B}
abstractNote = {Freeze-dried pancreas sections from obese hyperglycemic mice were subjected to proton bombardment and the elemental contents in the ..beta.. cells and the exocrine part were obtained from the characteristic X-rays emitted. Quantitative data were provided for 18 different elements. The mole ratio between K and Na exceeded 10, implying that neither the sample preparation nor the irradiation had induced significant diffuse changes. With the demonstration of this high K/Na ratio it seems likely that also the ..beta.. cells are equipped with an efficient Na/sup +//K/sup +/ pump. The ..beta.. cells contained about 70 mmoles Cl per litre cell water. Observed amounts of Ca and Mg were equivalent to those previously recorded by electrothermal atomic absorption spectroscopy. The significant role of Zn for the storage of insulin was emphasized by the demonstration of 3 times as much of this element in the ..beta.. cells as compared with the exocrine pancreas. In addition, the sensitivity of the proton microprobe enabled measurements of various trace elements such as Rb, Cr, Cu, Al and Pb not previously demonstrated in the pancreatic ..beta.. cells.}
journal = {Biomed. Biochim. Acta; (Germany, Federal Republic of)}
volume = {44:1}
place = {Germany}
year = {1985}
month = {Jan}
}