Abstract
Nuclear microprobe analysis was applied to the study of elemental distribution in brains sections of patients with a diagnosis of Alzheimer's disease. Stained and nonstained cryosections were studied. The work carried out shows that serious elemental losses follow the sample staining procedure. Major losses occurred in a simple rinse of the tissue section, probably reducing most of the in-vivo gradients, which show that generally very little information can be gained from stained sections. However, in many cases stained sections are compulsory because of the requirement to recognize the area which is to be studied. All the elemental maps obtained for the neurofibrillary deposits indicate a localized concentration for Si and probably also Al, associated with the senile plaque core. Neither of these elements were found in the staining solutions used. The validity of the results is discussed as well as the possible link of Al and/or Si in the development of Alzheimer's desease. (orig.).
Pinheiro, T;
[1]
Tapper, U A.S.;
[2]
Sturesson, K;
Brun, A
[3]
- CEC-JRC, Central Bureau for Nuclear Measurements, Geel (Belgium)
- Dept. of Nuclear Physics, Lund Inst. of Science and Tech. (Sweden)
- Div. of Neuropathology, Dept. of Pathology, Lund University Hospital (Sweden)
Citation Formats
Pinheiro, T, Tapper, U A.S., Sturesson, K, and Brun, A.
Experimental investigations into sample preparation of Alzheimer tissue specimens for nuclear microprobe analysis.
Netherlands: N. p.,
1991.
Web.
Pinheiro, T, Tapper, U A.S., Sturesson, K, & Brun, A.
Experimental investigations into sample preparation of Alzheimer tissue specimens for nuclear microprobe analysis.
Netherlands.
Pinheiro, T, Tapper, U A.S., Sturesson, K, and Brun, A.
1991.
"Experimental investigations into sample preparation of Alzheimer tissue specimens for nuclear microprobe analysis."
Netherlands.
@misc{etde_5418044,
title = {Experimental investigations into sample preparation of Alzheimer tissue specimens for nuclear microprobe analysis}
author = {Pinheiro, T, Tapper, U A.S., Sturesson, K, and Brun, A}
abstractNote = {Nuclear microprobe analysis was applied to the study of elemental distribution in brains sections of patients with a diagnosis of Alzheimer's disease. Stained and nonstained cryosections were studied. The work carried out shows that serious elemental losses follow the sample staining procedure. Major losses occurred in a simple rinse of the tissue section, probably reducing most of the in-vivo gradients, which show that generally very little information can be gained from stained sections. However, in many cases stained sections are compulsory because of the requirement to recognize the area which is to be studied. All the elemental maps obtained for the neurofibrillary deposits indicate a localized concentration for Si and probably also Al, associated with the senile plaque core. Neither of these elements were found in the staining solutions used. The validity of the results is discussed as well as the possible link of Al and/or Si in the development of Alzheimer's desease. (orig.).}
journal = []
volume = {54:1-3}
place = {Netherlands}
year = {1991}
month = {Mar}
}
title = {Experimental investigations into sample preparation of Alzheimer tissue specimens for nuclear microprobe analysis}
author = {Pinheiro, T, Tapper, U A.S., Sturesson, K, and Brun, A}
abstractNote = {Nuclear microprobe analysis was applied to the study of elemental distribution in brains sections of patients with a diagnosis of Alzheimer's disease. Stained and nonstained cryosections were studied. The work carried out shows that serious elemental losses follow the sample staining procedure. Major losses occurred in a simple rinse of the tissue section, probably reducing most of the in-vivo gradients, which show that generally very little information can be gained from stained sections. However, in many cases stained sections are compulsory because of the requirement to recognize the area which is to be studied. All the elemental maps obtained for the neurofibrillary deposits indicate a localized concentration for Si and probably also Al, associated with the senile plaque core. Neither of these elements were found in the staining solutions used. The validity of the results is discussed as well as the possible link of Al and/or Si in the development of Alzheimer's desease. (orig.).}
journal = []
volume = {54:1-3}
place = {Netherlands}
year = {1991}
month = {Mar}
}