You need JavaScript to view this

Radioimmunoassay of bovine, ovine and porcine luteinizing hormone with a monoclonal antibody and a human tracer

Journal Article:

Abstract

A radioimmunoassay for bovine (bLH), ovine (oLH) and porcine (pLH) luteinizing hormone was developed using a human [sup 125]ILH tracer from a commercial kit and a monoclonal antibody (518B7) specific for LH but with low species specificity. Standard curves demonstrated similar binding kinetics when bLH, oLH and pLH were incubated with tracer and antibody for 2 h at room temperature. A 30-min delay in the addition of the tracer gave sufficient sensitivity when analysing pLH. Separation of antibody-bound LH from free hormone was achieved by using second antibody-coated micro Sepharose beads. The assay was validated and the performance compared with that of an RIA currently in use for determination of bLH (coefficient of correlation: 0.99 and 0.98). Regardless of the standards used, intra-assay coefficients of variation were <10% for LH concentrations exceeding 1 [mu]g/L. The inter-assay coefficients of variation were <15%. The assay was used for clinical evaluation demonstrating the pre-ovulatory LH surge in two cyclic cows, LH pulsatility in an oophorectomized ewe and LH response to GnRH injection in a boar. (au) (7 refs.).
Publication Date:
Jan 01, 1993
Product Type:
Journal Article
Reference Number:
AIX-25-017945; EDB-94-040155
Resource Relation:
Journal Name: Acta Veterinaria Scandinavica; (Denmark); Journal Volume: 34:3
Subject:
60 APPLIED LIFE SCIENCES; COWS; LH; RADIOIMMUNOASSAY; SHEEP; IODINE 125; MONOCLONAL ANTIBODIES; SENSITIVITY; SPECIFICITY; ANIMALS; ANTIBODIES; BETA DECAY RADIOISOTOPES; BIOASSAY; CATTLE; DAYS LIVING RADIOISOTOPES; DIAGNOSTIC TECHNIQUES; DOMESTIC ANIMALS; ELECTRON CAPTURE RADIOISOTOPES; GONADOTROPINS; HORMONES; IMMUNOASSAY; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PEPTIDE HORMONES; PITUITARY HORMONES; PROTEINS; RADIOASSAY; RADIOIMMUNODETECTION; RADIOISOTOPES; RUMINANTS; TRACER TECHNIQUES; VERTEBRATES; 553006* - Agriculture & Food Technology- Other Agricultural Applications- (1987-)
OSTI ID:
5413244
Country of Origin:
Denmark
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0044-605X; CODEN: AVSCA7
Submitting Site:
DKN
Size:
Pages: 255-262
Announcement Date:

Journal Article:

Citation Formats

Fosberg, M, Tagle, R, Madej, A, Molina, J R, and Carlsson, M -A. Radioimmunoassay of bovine, ovine and porcine luteinizing hormone with a monoclonal antibody and a human tracer. Denmark: N. p., 1993. Web.
Fosberg, M, Tagle, R, Madej, A, Molina, J R, & Carlsson, M -A. Radioimmunoassay of bovine, ovine and porcine luteinizing hormone with a monoclonal antibody and a human tracer. Denmark.
Fosberg, M, Tagle, R, Madej, A, Molina, J R, and Carlsson, M -A. 1993. "Radioimmunoassay of bovine, ovine and porcine luteinizing hormone with a monoclonal antibody and a human tracer." Denmark.
@misc{etde_5413244,
title = {Radioimmunoassay of bovine, ovine and porcine luteinizing hormone with a monoclonal antibody and a human tracer}
author = {Fosberg, M, Tagle, R, Madej, A, Molina, J R, and Carlsson, M -A}
abstractNote = {A radioimmunoassay for bovine (bLH), ovine (oLH) and porcine (pLH) luteinizing hormone was developed using a human [sup 125]ILH tracer from a commercial kit and a monoclonal antibody (518B7) specific for LH but with low species specificity. Standard curves demonstrated similar binding kinetics when bLH, oLH and pLH were incubated with tracer and antibody for 2 h at room temperature. A 30-min delay in the addition of the tracer gave sufficient sensitivity when analysing pLH. Separation of antibody-bound LH from free hormone was achieved by using second antibody-coated micro Sepharose beads. The assay was validated and the performance compared with that of an RIA currently in use for determination of bLH (coefficient of correlation: 0.99 and 0.98). Regardless of the standards used, intra-assay coefficients of variation were <10% for LH concentrations exceeding 1 [mu]g/L. The inter-assay coefficients of variation were <15%. The assay was used for clinical evaluation demonstrating the pre-ovulatory LH surge in two cyclic cows, LH pulsatility in an oophorectomized ewe and LH response to GnRH injection in a boar. (au) (7 refs.).}
journal = {Acta Veterinaria Scandinavica; (Denmark)}
volume = {34:3}
journal type = {AC}
place = {Denmark}
year = {1993}
month = {Jan}
}