Abstract
The phosphate metabolites, adenosine diphosphate (ADP), inorganic phosphate (P/sub i/), and adenosine triphosphate (ATP), are potentially important regulators of mitochondrial respiration in vivo. However, previous studies on the heart in vivo and in vitro have not consistently demonstrated an appropriate correlation between the concentration of these phosphate metabolites and moderate changes in work and respiration. Recently, mitochondrial NAD(P)H levels have been proposed as a potential regulator of cardiac respiration during alterations in work output. In order to understand better the mechanism of respiratory control under these conditions, we investigated the relationship between the phosphate metabolites, the NAD(P)H levels, and oxygen consumption (Q/sub O(sub 2)/) in the isovolumic perfused rat heart during alterations in work output with pacing. ATP, creatine phosphate (CrP), P/sub i/ and intracellular pH were measured using /sup 31/P NMR. Mitochondrial NAD(P)H levels were monitored using spectrofluorometric techniques. 33 refs.; 3 figs.; 2 tabs.
Citation Formats
Katz, L A, Koretsky, A P, and Balaban, R S.
Respiratory control in the glucose perfused heart. A /sup 31/P NMR and NADH fluorescence study.
Netherlands: N. p.,
1987.
Web.
doi:10.1016/0014-5793(87)80939-0.
Katz, L A, Koretsky, A P, & Balaban, R S.
Respiratory control in the glucose perfused heart. A /sup 31/P NMR and NADH fluorescence study.
Netherlands.
https://doi.org/10.1016/0014-5793(87)80939-0
Katz, L A, Koretsky, A P, and Balaban, R S.
1987.
"Respiratory control in the glucose perfused heart. A /sup 31/P NMR and NADH fluorescence study."
Netherlands.
https://doi.org/10.1016/0014-5793(87)80939-0.
@misc{etde_5407688,
title = {Respiratory control in the glucose perfused heart. A /sup 31/P NMR and NADH fluorescence study}
author = {Katz, L A, Koretsky, A P, and Balaban, R S}
abstractNote = {The phosphate metabolites, adenosine diphosphate (ADP), inorganic phosphate (P/sub i/), and adenosine triphosphate (ATP), are potentially important regulators of mitochondrial respiration in vivo. However, previous studies on the heart in vivo and in vitro have not consistently demonstrated an appropriate correlation between the concentration of these phosphate metabolites and moderate changes in work and respiration. Recently, mitochondrial NAD(P)H levels have been proposed as a potential regulator of cardiac respiration during alterations in work output. In order to understand better the mechanism of respiratory control under these conditions, we investigated the relationship between the phosphate metabolites, the NAD(P)H levels, and oxygen consumption (Q/sub O(sub 2)/) in the isovolumic perfused rat heart during alterations in work output with pacing. ATP, creatine phosphate (CrP), P/sub i/ and intracellular pH were measured using /sup 31/P NMR. Mitochondrial NAD(P)H levels were monitored using spectrofluorometric techniques. 33 refs.; 3 figs.; 2 tabs.}
doi = {10.1016/0014-5793(87)80939-0}
journal = []
volume = {221:2}
journal type = {AC}
place = {Netherlands}
year = {1987}
month = {Sep}
}
title = {Respiratory control in the glucose perfused heart. A /sup 31/P NMR and NADH fluorescence study}
author = {Katz, L A, Koretsky, A P, and Balaban, R S}
abstractNote = {The phosphate metabolites, adenosine diphosphate (ADP), inorganic phosphate (P/sub i/), and adenosine triphosphate (ATP), are potentially important regulators of mitochondrial respiration in vivo. However, previous studies on the heart in vivo and in vitro have not consistently demonstrated an appropriate correlation between the concentration of these phosphate metabolites and moderate changes in work and respiration. Recently, mitochondrial NAD(P)H levels have been proposed as a potential regulator of cardiac respiration during alterations in work output. In order to understand better the mechanism of respiratory control under these conditions, we investigated the relationship between the phosphate metabolites, the NAD(P)H levels, and oxygen consumption (Q/sub O(sub 2)/) in the isovolumic perfused rat heart during alterations in work output with pacing. ATP, creatine phosphate (CrP), P/sub i/ and intracellular pH were measured using /sup 31/P NMR. Mitochondrial NAD(P)H levels were monitored using spectrofluorometric techniques. 33 refs.; 3 figs.; 2 tabs.}
doi = {10.1016/0014-5793(87)80939-0}
journal = []
volume = {221:2}
journal type = {AC}
place = {Netherlands}
year = {1987}
month = {Sep}
}