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Antibody response to the lipopolysaccharide and protein antigens of Salmonella typhi during typhoid infection. I. Measurement of serum antibodies by radioimmunoassay

Journal Article:

Abstract

Serum antibody responses to the lipopolysaccharide and protein antigens of S. typhi in typhoid patients were studied using a solid-phase radioimmunoassay technique with /sup 125/I labelled anti-immunoglobulin antibody. Sera from 24 adult typhoid patients and 20 non-typhoid adult controls were compared. As a group, sera from typhoid patients showed increased IgA, IgG and IgM immunoglobulin levels and gave significantly higher anti-LPS and anti-protein antibody titres in all three major immunoglobulin classes than did non-typhoid controls. Levels of antibodies against LPS or protein in sera of typhoid patients were highly variable with a skew distribution. A good correlation was found between antibody titres to the LPS antigen and those to a protein antigen. No correlation, however, was found between the anti-LPS antibody titres measured by radioimmunoassay and the anti-O antibody titres measured by the Widal agglutination test. Titration of anti-LPS or anti-protein antibodies by radioimmunoassay was found to be more sensitive and specific than Widal test for the serological diagnosis of typhoid fever. The advantages of measuring antibody response by radioimmunoassay over conventional Widal test are discussed.
Authors:
Tsang, R S.W.; Chau, P Y; Lam, S K; [1]  La Brooy, J T; Rowley, D [2] 
  1. Hong Kong Univ.
  2. Adelaide Univ. (Australia)
Publication Date:
Dec 01, 1981
Product Type:
Journal Article
Reference Number:
AIX-13-680368; EDB-82-115901
Resource Relation:
Journal Name: Clin. Exp. Immunol.; (United Kingdom); Journal Volume: 46:3
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; BLOOD SERUM; RADIOIMMUNOASSAY; IODINE 125; TYPHOID; DIAGNOSTIC TECHNIQUES; ANTIBODIES; ANTIGEN-ANTIBODY REACTIONS; IMMUNOGLOBULINS; LIPOPOLYSACCHARIDES; SALMONELLA; BACTERIA; BACTERIAL DISEASES; BETA DECAY RADIOISOTOPES; CARBOHYDRATES; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; GLOBULINS; INFECTIOUS DISEASES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LIPIDS; MICROORGANISMS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; POLYSACCHARIDES; PROTEINS; RADIOASSAY; RADIOISOTOPES; SACCHARIDES; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5405650
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: CEXIA
Submitting Site:
HEDB
Size:
Pages: 508-514
Announcement Date:

Journal Article:

Citation Formats

Tsang, R S.W., Chau, P Y, Lam, S K, La Brooy, J T, and Rowley, D. Antibody response to the lipopolysaccharide and protein antigens of Salmonella typhi during typhoid infection. I. Measurement of serum antibodies by radioimmunoassay. United Kingdom: N. p., 1981. Web.
Tsang, R S.W., Chau, P Y, Lam, S K, La Brooy, J T, & Rowley, D. Antibody response to the lipopolysaccharide and protein antigens of Salmonella typhi during typhoid infection. I. Measurement of serum antibodies by radioimmunoassay. United Kingdom.
Tsang, R S.W., Chau, P Y, Lam, S K, La Brooy, J T, and Rowley, D. 1981. "Antibody response to the lipopolysaccharide and protein antigens of Salmonella typhi during typhoid infection. I. Measurement of serum antibodies by radioimmunoassay." United Kingdom.
@misc{etde_5405650,
title = {Antibody response to the lipopolysaccharide and protein antigens of Salmonella typhi during typhoid infection. I. Measurement of serum antibodies by radioimmunoassay}
author = {Tsang, R S.W., Chau, P Y, Lam, S K, La Brooy, J T, and Rowley, D}
abstractNote = {Serum antibody responses to the lipopolysaccharide and protein antigens of S. typhi in typhoid patients were studied using a solid-phase radioimmunoassay technique with /sup 125/I labelled anti-immunoglobulin antibody. Sera from 24 adult typhoid patients and 20 non-typhoid adult controls were compared. As a group, sera from typhoid patients showed increased IgA, IgG and IgM immunoglobulin levels and gave significantly higher anti-LPS and anti-protein antibody titres in all three major immunoglobulin classes than did non-typhoid controls. Levels of antibodies against LPS or protein in sera of typhoid patients were highly variable with a skew distribution. A good correlation was found between antibody titres to the LPS antigen and those to a protein antigen. No correlation, however, was found between the anti-LPS antibody titres measured by radioimmunoassay and the anti-O antibody titres measured by the Widal agglutination test. Titration of anti-LPS or anti-protein antibodies by radioimmunoassay was found to be more sensitive and specific than Widal test for the serological diagnosis of typhoid fever. The advantages of measuring antibody response by radioimmunoassay over conventional Widal test are discussed.}
journal = {Clin. Exp. Immunol.; (United Kingdom)}
volume = {46:3}
journal type = {AC}
place = {United Kingdom}
year = {1981}
month = {Dec}
}