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Identification of the D-1 dopamine receptor subunit in rat striatum after photoaffinity labeling

Journal Article:

Abstract

When rat striatal membranes, photolabeled with (/sup 3/H)dopamine under assay conditions similar to those used for dopamine-sensitive adenylate cyclase, were subjected to sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis, several radioactively labeled bands appeared. Labeling of these bands was reduced in the presence of non-radioactive dopamine during photolysis, but was unaffected by the presence of sulpiride. Haloperidol preferentially reduced the labeling of the main band which had a molecular weight of about 57,000 rather than the other weakly labeled bands. Labeling of this 57,000 dalton protein was not apparent when rat cerebellar membranes were used and was markedly eliminated by kainic acid-induced lesions that destroyed the intrastriatal nerve cell bodies. These results indicate that this 57,000 dalton protein is the binding subunit of the D-1 dopamine receptor.
Authors:
Kuno, T; Tanaka, C [1] 
  1. Kobe Univ. (Japan). School of Medicine
Publication Date:
Dec 28, 1981
Product Type:
Journal Article
Reference Number:
AIX-13-671404; EDB-82-104941
Resource Relation:
Journal Name: Brain Res.; (Netherlands); Journal Volume: 230:1-2
Subject:
59 BASIC BIOLOGICAL SCIENCES; BRAIN; DOPAMINE; RECEPTORS; LABELLED COMPOUNDS; RADIONUCLIDE KINETICS; RATS; TRITIUM; AMINES; ANIMALS; AROMATICS; AUTONOMIC NERVOUS SYSTEM AGENTS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CARDIOTONICS; CARDIOVASCULAR AGENTS; CENTRAL NERVOUS SYSTEM; DRUGS; HYDROGEN ISOTOPES; HYDROXY COMPOUNDS; ISOTOPES; LIGHT NUCLEI; MAMMALS; NERVOUS SYSTEM; NEUROREGULATORS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PHENOLS; POLYPHENOLS; RADIOISOTOPES; RODENTS; SYMPATHOMIMETICS; VERTEBRATES; YEARS LIVING RADIOISOTOPES; 550501* - Metabolism- Tracer Techniques
OSTI ID:
5405418
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BRREA
Submitting Site:
HEDB
Size:
Pages: 417-421
Announcement Date:

Journal Article:

Citation Formats

Kuno, T, and Tanaka, C. Identification of the D-1 dopamine receptor subunit in rat striatum after photoaffinity labeling. Netherlands: N. p., 1981. Web.
Kuno, T, & Tanaka, C. Identification of the D-1 dopamine receptor subunit in rat striatum after photoaffinity labeling. Netherlands.
Kuno, T, and Tanaka, C. 1981. "Identification of the D-1 dopamine receptor subunit in rat striatum after photoaffinity labeling." Netherlands.
@misc{etde_5405418,
title = {Identification of the D-1 dopamine receptor subunit in rat striatum after photoaffinity labeling}
author = {Kuno, T, and Tanaka, C}
abstractNote = {When rat striatal membranes, photolabeled with (/sup 3/H)dopamine under assay conditions similar to those used for dopamine-sensitive adenylate cyclase, were subjected to sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis, several radioactively labeled bands appeared. Labeling of these bands was reduced in the presence of non-radioactive dopamine during photolysis, but was unaffected by the presence of sulpiride. Haloperidol preferentially reduced the labeling of the main band which had a molecular weight of about 57,000 rather than the other weakly labeled bands. Labeling of this 57,000 dalton protein was not apparent when rat cerebellar membranes were used and was markedly eliminated by kainic acid-induced lesions that destroyed the intrastriatal nerve cell bodies. These results indicate that this 57,000 dalton protein is the binding subunit of the D-1 dopamine receptor.}
journal = {Brain Res.; (Netherlands)}
volume = {230:1-2}
journal type = {AC}
place = {Netherlands}
year = {1981}
month = {Dec}
}