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Double-strand break repair and G/sub 2/ block in Chinese hamster ovary cells and their radiosensitive mutants

Journal Article:

Abstract

Two X-ray-sensitive mutants of the CHO K1 cell line were examined for their cell-cycle progression after irradiation with ..gamma..-rays, and for their ability to rejoin double-strand breaks (DSBs) as detected by neutral filter elution. Both mutants were impaired in DSB rejoining and both were irreversibly blocked in the G/sub 2/ phase of the cell cycle as determined by cytofluorometry. From one mutant the authors have isolated several revertants. The revertants stem from genomic DNA transfection experiments and may have been caused by gene uptake. All revertants survived ..gamma..-irradiation as did the wild-type CHO line. One of them has been examined for its ability to rejoin DSBs and was found to be similar to the wild type.
Authors:
Weibezahn, K F; Lohrer, H; Herrlich, P [1] 
  1. Kernforschungszentrum Karlsruhe G.m.b.H. (Germany, F.R.). Inst. fuer Genetik und Toxikologie
Publication Date:
May 01, 1985
Product Type:
Journal Article
Reference Number:
AIX-16-063677; EDB-85-149823
Resource Relation:
Journal Name: Mutat. Res.; (Netherlands); Journal Volume: 145:3
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CHO CELLS; GENETIC RADIATION EFFECTS; STRAND BREAKS; DNA REPAIR; CELL CYCLE; GAMMA RADIATION; RADIOSENSITIVITY; REVERTANTS; X RADIATION; ANIMAL CELLS; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; ELECTROMAGNETIC RADIATION; GENETIC EFFECTS; IONIZING RADIATIONS; MUTANTS; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; 560111* - Radiation Effects on Biochemicals- In Vitro- (-1987)
OSTI ID:
5378367
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: MUREA
Submitting Site:
HEDB
Size:
Pages: 177-183
Announcement Date:

Journal Article:

Citation Formats

Weibezahn, K F, Lohrer, H, and Herrlich, P. Double-strand break repair and G/sub 2/ block in Chinese hamster ovary cells and their radiosensitive mutants. Netherlands: N. p., 1985. Web.
Weibezahn, K F, Lohrer, H, & Herrlich, P. Double-strand break repair and G/sub 2/ block in Chinese hamster ovary cells and their radiosensitive mutants. Netherlands.
Weibezahn, K F, Lohrer, H, and Herrlich, P. 1985. "Double-strand break repair and G/sub 2/ block in Chinese hamster ovary cells and their radiosensitive mutants." Netherlands.
@misc{etde_5378367,
title = {Double-strand break repair and G/sub 2/ block in Chinese hamster ovary cells and their radiosensitive mutants}
author = {Weibezahn, K F, Lohrer, H, and Herrlich, P}
abstractNote = {Two X-ray-sensitive mutants of the CHO K1 cell line were examined for their cell-cycle progression after irradiation with ..gamma..-rays, and for their ability to rejoin double-strand breaks (DSBs) as detected by neutral filter elution. Both mutants were impaired in DSB rejoining and both were irreversibly blocked in the G/sub 2/ phase of the cell cycle as determined by cytofluorometry. From one mutant the authors have isolated several revertants. The revertants stem from genomic DNA transfection experiments and may have been caused by gene uptake. All revertants survived ..gamma..-irradiation as did the wild-type CHO line. One of them has been examined for its ability to rejoin DSBs and was found to be similar to the wild type.}
journal = {Mutat. Res.; (Netherlands)}
volume = {145:3}
journal type = {AC}
place = {Netherlands}
year = {1985}
month = {May}
}