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Core nucleosomes by digestion of reconstructed histone-DNA complexes

Abstract

Reconstructed complexes of the inner histones (H2A, H2B, H3, H4) and a variety of DNAs were digested with micrococcal nuclease to yield very homogeneous populations of core nucleosomes (..nu../sub 1/). Nucleosomes containing Micrococcus luteus DNA (72% G+C); chicken DNA (43% G+C), Clostridium perfringens DNA (29% G+C); or poly(dA-dT).poly(dA-dT) have been examined by circular dichroism, thermal denaturation, electron microscopy, and DNAse I digestion. Circular dichroism spectra of all particles show a typically suppressed ellipticity at 260 to 280 nm and a prominent ..cap alpha..-helix signal at 222 nm. All particles show biphasic melting except ..nu../sub 1/(dA-dT), which show three prominent melting transitions at ionic strength less than or equal to 1 mM. DNAse I digestion of ..nu../sub 1/ (dA-dT) produces a ladder of DNA fragments differing in length by one base residue. ..nu../sub 1/ (dA-dT) contain 146 base pairs of DNA and exhibit an average DNA helix pitch of 10.4 to 10.5 bases per turn. There appear to be two regions of different DNA pitch within ..nu../sub 1/ (dA-dT). It is suggested that the two regions of DNA pitch might correspond to the two regions of the melting profiles.
Publication Date:
Apr 01, 1979
Product Type:
Journal Article
Reference Number:
ERA-05-029654; EDB-80-085847
Resource Relation:
Journal Name: Nucleic Acids Res.; (United Kingdom); Journal Volume: 6:4
Subject:
59 BASIC BIOLOGICAL SCIENCES; COMPLEXES; STRUCTURAL CHEMICAL ANALYSIS; DNA; HISTONES; DICHROISM; DNA-ASE; ELECTRON MICROSCOPY; PROTEIN DENATURATION; ENZYMES; ESTERASES; HYDROLASES; MICROSCOPY; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PHOSPHATASES; PROTEINS; 550200* - Biochemistry; 550300 - Cytology
OSTI ID:
5374919
Research Organizations:
Oak Ridge National Lab., TN
Country of Origin:
United Kingdom
Language:
English
Contract Number:
W-7405-ENG-26
Other Identifying Numbers:
Journal ID: CODEN: NARHA
Submitting Site:
ORNL
Size:
Pages: 1449-1466
Announcement Date:

Citation Formats

Bryan, P N, Wright, E B, and Olins, D E. Core nucleosomes by digestion of reconstructed histone-DNA complexes. United Kingdom: N. p., 1979. Web. doi:10.1093/nar/6.4.1449.
Bryan, P N, Wright, E B, & Olins, D E. Core nucleosomes by digestion of reconstructed histone-DNA complexes. United Kingdom. doi:10.1093/nar/6.4.1449.
Bryan, P N, Wright, E B, and Olins, D E. 1979. "Core nucleosomes by digestion of reconstructed histone-DNA complexes." United Kingdom. doi:10.1093/nar/6.4.1449. https://www.osti.gov/servlets/purl/10.1093/nar/6.4.1449.
@misc{etde_5374919,
title = {Core nucleosomes by digestion of reconstructed histone-DNA complexes}
author = {Bryan, P N, Wright, E B, and Olins, D E}
abstractNote = {Reconstructed complexes of the inner histones (H2A, H2B, H3, H4) and a variety of DNAs were digested with micrococcal nuclease to yield very homogeneous populations of core nucleosomes (..nu../sub 1/). Nucleosomes containing Micrococcus luteus DNA (72% G+C); chicken DNA (43% G+C), Clostridium perfringens DNA (29% G+C); or poly(dA-dT).poly(dA-dT) have been examined by circular dichroism, thermal denaturation, electron microscopy, and DNAse I digestion. Circular dichroism spectra of all particles show a typically suppressed ellipticity at 260 to 280 nm and a prominent ..cap alpha..-helix signal at 222 nm. All particles show biphasic melting except ..nu../sub 1/(dA-dT), which show three prominent melting transitions at ionic strength less than or equal to 1 mM. DNAse I digestion of ..nu../sub 1/ (dA-dT) produces a ladder of DNA fragments differing in length by one base residue. ..nu../sub 1/ (dA-dT) contain 146 base pairs of DNA and exhibit an average DNA helix pitch of 10.4 to 10.5 bases per turn. There appear to be two regions of different DNA pitch within ..nu../sub 1/ (dA-dT). It is suggested that the two regions of DNA pitch might correspond to the two regions of the melting profiles.}
doi = {10.1093/nar/6.4.1449}
journal = {Nucleic Acids Res.; (United Kingdom)}
volume = {6:4}
journal type = {AC}
place = {United Kingdom}
year = {1979}
month = {Apr}
}