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Photochemical (PUVA) treatment of isolated rat islets

Conference:

Abstract

Isolated rat islets were irradiated with long-wave ultraviolet light alone or in combination with the photosensitizer 8-methoxypsoralen. The influence on specific beta cell functions was determined with the aim to find out experimental conditions which allow the use of such islets for transplantation. Short-term effects: Ultraviolet light affected (/sup 3/H)leucine incorporation into (pro)insulin (5 J/cm/sup 2/ : 53.8 %, 10 J/cm/sup 2/ : 41.0 % of the controls) and insulin release was slightly reduced. 8-methoxypsoralen enhanced the irradiation effect. Long-term effects: A restoration of irradiation-affected beta cell function was detected after 5 days of culture unless the dose exceeded 2 J/cm/sup 2/ (0.1 ..mu..M 8-methoxypsoralen) or 1 J/cm/sup 2/ (1 ..mu..M 8-methoxypsoralen). After functional restoration islets were used for transplantation experiments.
Authors:
Schmidt, S; Wilke, B; Kloeting, I [1] 
  1. Zentralinstitut fuer Diabetes, Karlsburg (German Democratic Republic)
Publication Date:
May 01, 1984
Product Type:
Conference
Report Number:
CONF-8309352-
Reference Number:
AIX-16-049836; EDB-85-134843
Resource Relation:
Journal Name: Biomed. Biochim. Acta; (Germany, Federal Republic of); Journal Volume: 43:5; Conference: 11. international Karlsburg symposium on problems of diabetes, Karlsburg, Germany D.R., 19 Sep 1983
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; PANCREAS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL RECOVERY; INSULIN; NEAR ULTRAVIOLET RADIATION; PHOTOSENSITIVITY; PSORALEN; RATS; SOMATIC CELLS; TRANSPLANTS; ANIMAL CELLS; ANIMALS; ANTICOAGULANTS; BIOLOGICAL EFFECTS; BODY; COUMARINS; DIGESTIVE SYSTEM; DRUGS; ELECTROMAGNETIC RADIATION; ENDOCRINE GLANDS; GLANDS; HEMATOLOGIC AGENTS; HETEROCYCLIC COMPOUNDS; HORMONES; MAMMALS; ORGANIC COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; ORGANS; PEPTIDE HORMONES; RADIATION EFFECTS; RADIATIONS; RECOVERY; RODENTS; SENSITIVITY; ULTRAVIOLET RADIATION; VERTEBRATES; 560121* - Radiation Effects on Cells- External Source- (-1987)
OSTI ID:
5367885
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BBIAD
Submitting Site:
HEDB
Size:
Pages: 703-710
Announcement Date:

Conference:

Citation Formats

Schmidt, S, Wilke, B, and Kloeting, I. Photochemical (PUVA) treatment of isolated rat islets. Germany: N. p., 1984. Web.
Schmidt, S, Wilke, B, & Kloeting, I. Photochemical (PUVA) treatment of isolated rat islets. Germany.
Schmidt, S, Wilke, B, and Kloeting, I. 1984. "Photochemical (PUVA) treatment of isolated rat islets." Germany.
@misc{etde_5367885,
title = {Photochemical (PUVA) treatment of isolated rat islets}
author = {Schmidt, S, Wilke, B, and Kloeting, I}
abstractNote = {Isolated rat islets were irradiated with long-wave ultraviolet light alone or in combination with the photosensitizer 8-methoxypsoralen. The influence on specific beta cell functions was determined with the aim to find out experimental conditions which allow the use of such islets for transplantation. Short-term effects: Ultraviolet light affected (/sup 3/H)leucine incorporation into (pro)insulin (5 J/cm/sup 2/ : 53.8 %, 10 J/cm/sup 2/ : 41.0 % of the controls) and insulin release was slightly reduced. 8-methoxypsoralen enhanced the irradiation effect. Long-term effects: A restoration of irradiation-affected beta cell function was detected after 5 days of culture unless the dose exceeded 2 J/cm/sup 2/ (0.1 ..mu..M 8-methoxypsoralen) or 1 J/cm/sup 2/ (1 ..mu..M 8-methoxypsoralen). After functional restoration islets were used for transplantation experiments.}
journal = {Biomed. Biochim. Acta; (Germany, Federal Republic of)}
volume = {43:5}
place = {Germany}
year = {1984}
month = {May}
}