Abstract
Two monoclonal antibodies were raised against human gamma interferon (IFN-..gamma..) derived from E. coli harboring the recombinant cDNA for IFN-..gamma.., and one against a synthetic peptide representing its C-terminus amino acid sequence of 20 residues. The monoclonal antibody against the synthetic peptide reacted either with IFN-..gamma.. or the synthetic peptide. One monoclonal anti-IFN-..gamma.. did not react with the synthetic peptide, while the other showed a weak binding with the peptide. A 2-site '1-step' radioimmunoassay was developed. The assay was rapid with a sensitivity capable of detecting a few ng/ml of IFN-..gamma...
Tanaka, E;
Imai, M;
Usuda, S;
Tachibana, K;
Okamoto, H;
Ohike, Y;
Nakamura, T;
Miyakawa, Y;
Mayumi, M
[1]
- Jichi Medical School, Minamikawachi, Tochigi (Japan)
Citation Formats
Tanaka, E, Imai, M, Usuda, S, Tachibana, K, Okamoto, H, Ohike, Y, Nakamura, T, Miyakawa, Y, and Mayumi, M.
Two-site sandwich radioimmunoassay of human gamma interferon with monoclonal antibodies.
Netherlands: N. p.,
1985.
Web.
Tanaka, E, Imai, M, Usuda, S, Tachibana, K, Okamoto, H, Ohike, Y, Nakamura, T, Miyakawa, Y, & Mayumi, M.
Two-site sandwich radioimmunoassay of human gamma interferon with monoclonal antibodies.
Netherlands.
Tanaka, E, Imai, M, Usuda, S, Tachibana, K, Okamoto, H, Ohike, Y, Nakamura, T, Miyakawa, Y, and Mayumi, M.
1985.
"Two-site sandwich radioimmunoassay of human gamma interferon with monoclonal antibodies."
Netherlands.
@misc{etde_5357978,
title = {Two-site sandwich radioimmunoassay of human gamma interferon with monoclonal antibodies}
author = {Tanaka, E, Imai, M, Usuda, S, Tachibana, K, Okamoto, H, Ohike, Y, Nakamura, T, Miyakawa, Y, and Mayumi, M}
abstractNote = {Two monoclonal antibodies were raised against human gamma interferon (IFN-..gamma..) derived from E. coli harboring the recombinant cDNA for IFN-..gamma.., and one against a synthetic peptide representing its C-terminus amino acid sequence of 20 residues. The monoclonal antibody against the synthetic peptide reacted either with IFN-..gamma.. or the synthetic peptide. One monoclonal anti-IFN-..gamma.. did not react with the synthetic peptide, while the other showed a weak binding with the peptide. A 2-site '1-step' radioimmunoassay was developed. The assay was rapid with a sensitivity capable of detecting a few ng/ml of IFN-..gamma...}
journal = []
volume = {77:2}
journal type = {AC}
place = {Netherlands}
year = {1985}
month = {Mar}
}
title = {Two-site sandwich radioimmunoassay of human gamma interferon with monoclonal antibodies}
author = {Tanaka, E, Imai, M, Usuda, S, Tachibana, K, Okamoto, H, Ohike, Y, Nakamura, T, Miyakawa, Y, and Mayumi, M}
abstractNote = {Two monoclonal antibodies were raised against human gamma interferon (IFN-..gamma..) derived from E. coli harboring the recombinant cDNA for IFN-..gamma.., and one against a synthetic peptide representing its C-terminus amino acid sequence of 20 residues. The monoclonal antibody against the synthetic peptide reacted either with IFN-..gamma.. or the synthetic peptide. One monoclonal anti-IFN-..gamma.. did not react with the synthetic peptide, while the other showed a weak binding with the peptide. A 2-site '1-step' radioimmunoassay was developed. The assay was rapid with a sensitivity capable of detecting a few ng/ml of IFN-..gamma...}
journal = []
volume = {77:2}
journal type = {AC}
place = {Netherlands}
year = {1985}
month = {Mar}
}