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Two-site sandwich radioimmunoassay of human gamma interferon with monoclonal antibodies

Journal Article:

Abstract

Two monoclonal antibodies were raised against human gamma interferon (IFN-..gamma..) derived from E. coli harboring the recombinant cDNA for IFN-..gamma.., and one against a synthetic peptide representing its C-terminus amino acid sequence of 20 residues. The monoclonal antibody against the synthetic peptide reacted either with IFN-..gamma.. or the synthetic peptide. One monoclonal anti-IFN-..gamma.. did not react with the synthetic peptide, while the other showed a weak binding with the peptide. A 2-site '1-step' radioimmunoassay was developed. The assay was rapid with a sensitivity capable of detecting a few ng/ml of IFN-..gamma...
Authors:
Tanaka, E; Imai, M; Usuda, S; Tachibana, K; Okamoto, H; Ohike, Y; Nakamura, T; Miyakawa, Y; Mayumi, M [1] 
  1. Jichi Medical School, Minamikawachi, Tochigi (Japan)
Publication Date:
Mar 18, 1985
Product Type:
Journal Article
Reference Number:
AIX-16-061020; EDB-85-141775
Resource Relation:
Journal Name: J. Immunol. Methods; (Netherlands); Journal Volume: 77:2
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; INTERFERON; RADIOIMMUNOASSAY; ANTIGEN-ANTIBODY REACTIONS; MONOCLONAL ANTIBODIES; ANTIBODIES; ISOTOPE APPLICATIONS; RADIOASSAY; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5357978
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JIMMB
Submitting Site:
HEDB
Size:
Pages: 275-282
Announcement Date:

Journal Article:

Citation Formats

Tanaka, E, Imai, M, Usuda, S, Tachibana, K, Okamoto, H, Ohike, Y, Nakamura, T, Miyakawa, Y, and Mayumi, M. Two-site sandwich radioimmunoassay of human gamma interferon with monoclonal antibodies. Netherlands: N. p., 1985. Web.
Tanaka, E, Imai, M, Usuda, S, Tachibana, K, Okamoto, H, Ohike, Y, Nakamura, T, Miyakawa, Y, & Mayumi, M. Two-site sandwich radioimmunoassay of human gamma interferon with monoclonal antibodies. Netherlands.
Tanaka, E, Imai, M, Usuda, S, Tachibana, K, Okamoto, H, Ohike, Y, Nakamura, T, Miyakawa, Y, and Mayumi, M. 1985. "Two-site sandwich radioimmunoassay of human gamma interferon with monoclonal antibodies." Netherlands.
@misc{etde_5357978,
title = {Two-site sandwich radioimmunoassay of human gamma interferon with monoclonal antibodies}
author = {Tanaka, E, Imai, M, Usuda, S, Tachibana, K, Okamoto, H, Ohike, Y, Nakamura, T, Miyakawa, Y, and Mayumi, M}
abstractNote = {Two monoclonal antibodies were raised against human gamma interferon (IFN-..gamma..) derived from E. coli harboring the recombinant cDNA for IFN-..gamma.., and one against a synthetic peptide representing its C-terminus amino acid sequence of 20 residues. The monoclonal antibody against the synthetic peptide reacted either with IFN-..gamma.. or the synthetic peptide. One monoclonal anti-IFN-..gamma.. did not react with the synthetic peptide, while the other showed a weak binding with the peptide. A 2-site '1-step' radioimmunoassay was developed. The assay was rapid with a sensitivity capable of detecting a few ng/ml of IFN-..gamma...}
journal = {J. Immunol. Methods; (Netherlands)}
volume = {77:2}
journal type = {AC}
place = {Netherlands}
year = {1985}
month = {Mar}
}