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Protoplast isolation from Ulmus americana l. Pollen mother cells, tetrads, and microspores

Journal Article:

Abstract

Meiotic protoplasts of U. amerciana are potentially valuable for producing interspecific elm hybrids through protoplast fusion. Meiotic cells(pollen mother cells, tetrads, and microspores) were incubated in either a cellulase, hemicellylase and pectinase enzyme solution of a beta-1,3-glucanase (lainarinase) solution. Respective protoplast isolation frequencies for the three meiotic cell types were 100, 50, and 10%. Exclusion staining with 0.2% Evans blue and 0.1% methyl blue suggested protoplast viability. Some of the microspore protoplasts were vacuolated, which is an important condition for cell division. Although attempts of regenerating cell walls and inducing cell division were unsuccessful, these two problems may be superceded by protoplast fusion with more regenerative protoplasts.
Publication Date:
Jan 01, 1980
Product Type:
Journal Article
Reference Number:
EDB-82-122445
Resource Relation:
Journal Name: Can. J. For. Res.; (Canada); Journal Volume: 10:3
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; PLANT CELLS; CELL CULTURES; SEPARATION PROCESSES; CULTURE MEDIA; ENZYMES; HYBRIDIZATION; MICROSPORES; TREES; PLANTS; SPORES; 140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989); 550400 - Genetics
OSTI ID:
5355601
Research Organizations:
Lawrence Berkeley Lab., Univ. California, Berkeley, CA 94720, USA
Country of Origin:
Canada
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: CJFRA
Submitting Site:
HEDB
Size:
Pages: 284-289
Announcement Date:
Jun 01, 1982

Journal Article:

Citation Formats

Redenbaugh, M K, Westfall, R D, and Karnosky, D F. Protoplast isolation from Ulmus americana l. Pollen mother cells, tetrads, and microspores. Canada: N. p., 1980. Web.
Redenbaugh, M K, Westfall, R D, & Karnosky, D F. Protoplast isolation from Ulmus americana l. Pollen mother cells, tetrads, and microspores. Canada.
Redenbaugh, M K, Westfall, R D, and Karnosky, D F. 1980. "Protoplast isolation from Ulmus americana l. Pollen mother cells, tetrads, and microspores." Canada.
@misc{etde_5355601,
title = {Protoplast isolation from Ulmus americana l. Pollen mother cells, tetrads, and microspores}
author = {Redenbaugh, M K, Westfall, R D, and Karnosky, D F}
abstractNote = {Meiotic protoplasts of U. amerciana are potentially valuable for producing interspecific elm hybrids through protoplast fusion. Meiotic cells(pollen mother cells, tetrads, and microspores) were incubated in either a cellulase, hemicellylase and pectinase enzyme solution of a beta-1,3-glucanase (lainarinase) solution. Respective protoplast isolation frequencies for the three meiotic cell types were 100, 50, and 10%. Exclusion staining with 0.2% Evans blue and 0.1% methyl blue suggested protoplast viability. Some of the microspore protoplasts were vacuolated, which is an important condition for cell division. Although attempts of regenerating cell walls and inducing cell division were unsuccessful, these two problems may be superceded by protoplast fusion with more regenerative protoplasts.}
journal = {Can. J. For. Res.; (Canada)}
volume = {10:3}
journal type = {AC}
place = {Canada}
year = {1980}
month = {Jan}
}