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Simultaneous determination of the content of serotonin, dopamine, noradrenaline and adrenaline in pancreatic islets isolated from fed and starved mice

Journal Article:

Abstract

A highly sensitive double isotope method for the simultaneous determination of serotonin, dopamine, noradrenaline and adrenaline has been developed. Advantages and limitations of the method are discussed. The mentioned biogenic amines are all present in isolated pancreatic islet tissue from albino mice in concentrations ranging from approximately 5-30 ..mu..mol per kg wet weight (0.8-5 x 10/sup -3/ pmol/ng DNA). A somewhat higher content of these amines, especially dopamine, was found in pancreatic acinar tissue. The hypothesis that the impaired glucose-induced insulin secretion during starvation partly is caused by an increased content of biogenic amines in the pancreatic islets was not supported by our experiments which showed an unchanged islet content of these amines after 48 h starvation.
Authors:
Hansen, S E; Hedeskov, C J [1] 
  1. Copenhagen Univ. (Denmark)
Publication Date:
Jan 01, 1977
Product Type:
Journal Article
Reference Number:
AIX-09-354292; EDB-78-038501
Resource Relation:
Journal Name: Acta Endocrinol.; (Denmark); Journal Volume: 86
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; ADRENALINE; QUANTITATIVE CHEMICAL ANALYSIS; DOPAMINE; NORADRENALINE; SEROTONIN; CARBON 14 COMPOUNDS; DOUBLE LABELLING; MICE; PANCREAS; THIN-LAYER CHROMATOGRAPHY; TRITIUM COMPOUNDS; ADRENAL HORMONES; AMINES; ANIMALS; AROMATICS; AZOLES; BODY; CHEMICAL ANALYSIS; CHROMATOGRAPHY; DIGESTIVE SYSTEM; DRUGS; ENDOCRINE GLANDS; GLANDS; HETEROCYCLIC COMPOUNDS; HORMONES; HYDROXY COMPOUNDS; INDOLES; LABELLED COMPOUNDS; LABELLING; MAMMALS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PHENOLS; POLYPHENOLS; PYRROLES; RADIOACTIVE MATERIALS; RADIOPROTECTIVE SUBSTANCES; RESPONSE MODIFYING FACTORS; RODENTS; SEPARATION PROCESSES; STEROID HORMONES; SYMPATHOMIMETICS; TRYPTAMINES; VERTEBRATES; 400102* - Chemical & Spectral Procedures
OSTI ID:
5347219
Country of Origin:
Denmark
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: ACENA
Submitting Site:
INIS
Size:
Pages: 820-832
Announcement Date:
Feb 01, 1978

Journal Article:

Citation Formats

Hansen, S E, and Hedeskov, C J. Simultaneous determination of the content of serotonin, dopamine, noradrenaline and adrenaline in pancreatic islets isolated from fed and starved mice. Denmark: N. p., 1977. Web.
Hansen, S E, & Hedeskov, C J. Simultaneous determination of the content of serotonin, dopamine, noradrenaline and adrenaline in pancreatic islets isolated from fed and starved mice. Denmark.
Hansen, S E, and Hedeskov, C J. 1977. "Simultaneous determination of the content of serotonin, dopamine, noradrenaline and adrenaline in pancreatic islets isolated from fed and starved mice." Denmark.
@misc{etde_5347219,
title = {Simultaneous determination of the content of serotonin, dopamine, noradrenaline and adrenaline in pancreatic islets isolated from fed and starved mice}
author = {Hansen, S E, and Hedeskov, C J}
abstractNote = {A highly sensitive double isotope method for the simultaneous determination of serotonin, dopamine, noradrenaline and adrenaline has been developed. Advantages and limitations of the method are discussed. The mentioned biogenic amines are all present in isolated pancreatic islet tissue from albino mice in concentrations ranging from approximately 5-30 ..mu..mol per kg wet weight (0.8-5 x 10/sup -3/ pmol/ng DNA). A somewhat higher content of these amines, especially dopamine, was found in pancreatic acinar tissue. The hypothesis that the impaired glucose-induced insulin secretion during starvation partly is caused by an increased content of biogenic amines in the pancreatic islets was not supported by our experiments which showed an unchanged islet content of these amines after 48 h starvation.}
journal = {Acta Endocrinol.; (Denmark)}
volume = {86}
journal type = {AC}
place = {Denmark}
year = {1977}
month = {Jan}
}