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Measurement of IgG antibodies to house dust mite and grass pollen by a solid-phase radioimmunoassay

Abstract

A solid-phase radioimmunoassay was used to measure specific IgG antibodies to either Dermatophagoides pteronyssinus (DPT) or grass pollens. Radiolabelled protein A from Staphylococcus aureus (SpA) was used to determine the IgG antibodies attached to the microtubes. The binding of IgG from either normal or allergic sera to DPT-coated tubes was antigen specific and mediated by the Fab fragment of the immunoglobulin. IgG antibodies from non-allergic serum competed with IgE antibodies to DPT. IgE antibodies did not significantly interfere with the assay. Indeed heating a reaginic serum resulted in a striking reduction of the (/sup 125/I) anti- IgE binding to allergen-coated tubes without modifying the (/sup 125/I)-SpA binding. Furthermore, filtration of a reaginic serum through Sephacryl S-200 separated a peak of IgE antibodies. The solid phase method was more sensitive than a double-antibody technique employing the same DPT extract as labelled antigen. Non-allergic subjects had less IgG antibodies to DPT or grass pollens than allergic patients. In untreated patients, there was a good correlation between levels of IgG and IgE antibodies to grass pollens but not to DPT. Patients hyposensitized to house dust mite had on the average three times more specific IgG antibodies than untreated cases. (author).
Authors:
Delespesse, G; Debisschop, M J; Flament, J [1] 
  1. Hopital Saint Pierre, Louvain (Belgium). Lab. de Recherches de la Clinique Medicale
Publication Date:
Sep 01, 1979
Product Type:
Journal Article
Reference Number:
AIX-11-505213; EDB-80-091488
Resource Relation:
Journal Name: Clin. Allergy; (United Kingdom); Journal Volume: 9:5
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ALLERGY; DIAGNOSTIC TECHNIQUES; IMMUNOGLOBULINS; RADIOIMMUNOASSAY; ANTIBODIES; ANTIGEN-ANTIBODY REACTIONS; BLOOD SERUM; DUSTS; GRAMINEAE; IODINE 125; LABELLED COMPOUNDS; MAN; MITES; POLLEN; SPECIFICITY; ANIMALS; ARACHNIDS; ARTHROPODS; BETA DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; GAMETES; GERM CELLS; GLOBULINS; INTERMEDIATE MASS NUCLEI; INVERTEBRATES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PATHOLOGICAL CHANGES; PLANTS; PRIMATES; PROTEINS; RADIOASSAY; RADIOISOTOPES; TRACER TECHNIQUES; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5340871
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: CLAGB
Submitting Site:
INIS
Size:
Pages: 503-514
Announcement Date:

Citation Formats

Delespesse, G, Debisschop, M J, and Flament, J. Measurement of IgG antibodies to house dust mite and grass pollen by a solid-phase radioimmunoassay. United Kingdom: N. p., 1979. Web. doi:10.1111/j.1365-2222.1979.tb02515.x.
Delespesse, G, Debisschop, M J, & Flament, J. Measurement of IgG antibodies to house dust mite and grass pollen by a solid-phase radioimmunoassay. United Kingdom. doi:10.1111/j.1365-2222.1979.tb02515.x.
Delespesse, G, Debisschop, M J, and Flament, J. 1979. "Measurement of IgG antibodies to house dust mite and grass pollen by a solid-phase radioimmunoassay." United Kingdom. doi:10.1111/j.1365-2222.1979.tb02515.x. https://www.osti.gov/servlets/purl/10.1111/j.1365-2222.1979.tb02515.x.
@misc{etde_5340871,
title = {Measurement of IgG antibodies to house dust mite and grass pollen by a solid-phase radioimmunoassay}
author = {Delespesse, G, Debisschop, M J, and Flament, J}
abstractNote = {A solid-phase radioimmunoassay was used to measure specific IgG antibodies to either Dermatophagoides pteronyssinus (DPT) or grass pollens. Radiolabelled protein A from Staphylococcus aureus (SpA) was used to determine the IgG antibodies attached to the microtubes. The binding of IgG from either normal or allergic sera to DPT-coated tubes was antigen specific and mediated by the Fab fragment of the immunoglobulin. IgG antibodies from non-allergic serum competed with IgE antibodies to DPT. IgE antibodies did not significantly interfere with the assay. Indeed heating a reaginic serum resulted in a striking reduction of the (/sup 125/I) anti- IgE binding to allergen-coated tubes without modifying the (/sup 125/I)-SpA binding. Furthermore, filtration of a reaginic serum through Sephacryl S-200 separated a peak of IgE antibodies. The solid phase method was more sensitive than a double-antibody technique employing the same DPT extract as labelled antigen. Non-allergic subjects had less IgG antibodies to DPT or grass pollens than allergic patients. In untreated patients, there was a good correlation between levels of IgG and IgE antibodies to grass pollens but not to DPT. Patients hyposensitized to house dust mite had on the average three times more specific IgG antibodies than untreated cases. (author).}
doi = {10.1111/j.1365-2222.1979.tb02515.x}
journal = {Clin. Allergy; (United Kingdom)}
volume = {9:5}
journal type = {AC}
place = {United Kingdom}
year = {1979}
month = {Sep}
}