Abstract
Insulin-/sup 125/I antibody reaction was optimized by physical-chemical parameters. After the activation energies Esub(a) and Esub(d)-for association and association, respectively were calculated from the experimental data, the theoretical values of the reaction rate constants ksub(a) and ksub(d) were determined as well as equilibrium constants K. By means of the empirical formulae, the approximate incubation time for the RIA kit and maximal percent of insulin-/sup 125/I binding to antibody (%B) in relation to temperature were computed. The proposed method may be applied to the new antigen-binder systems preparation (new antibodies, shortening of the incubation time, temperature changes, influence of different ions and kind of buffer). (orig.) 891 MG/orig. 892 MBE.
Silberring, J;
Golda, W
[1]
- Akademia Medyczna, Krakow (Poland). Dept. of Endocrinology and Metabolism
Citation Formats
Silberring, J, and Golda, W.
RIA system programming by means of kinetic parameters.
Germany: N. p.,
1979.
Web.
Silberring, J, & Golda, W.
RIA system programming by means of kinetic parameters.
Germany.
Silberring, J, and Golda, W.
1979.
"RIA system programming by means of kinetic parameters."
Germany.
@misc{etde_5335571,
title = {RIA system programming by means of kinetic parameters}
author = {Silberring, J, and Golda, W}
abstractNote = {Insulin-/sup 125/I antibody reaction was optimized by physical-chemical parameters. After the activation energies Esub(a) and Esub(d)-for association and association, respectively were calculated from the experimental data, the theoretical values of the reaction rate constants ksub(a) and ksub(d) were determined as well as equilibrium constants K. By means of the empirical formulae, the approximate incubation time for the RIA kit and maximal percent of insulin-/sup 125/I binding to antibody (%B) in relation to temperature were computed. The proposed method may be applied to the new antigen-binder systems preparation (new antibodies, shortening of the incubation time, temperature changes, influence of different ions and kind of buffer). (orig.) 891 MG/orig. 892 MBE.}
journal = []
volume = {4:6}
journal type = {AC}
place = {Germany}
year = {1979}
month = {Dec}
}
title = {RIA system programming by means of kinetic parameters}
author = {Silberring, J, and Golda, W}
abstractNote = {Insulin-/sup 125/I antibody reaction was optimized by physical-chemical parameters. After the activation energies Esub(a) and Esub(d)-for association and association, respectively were calculated from the experimental data, the theoretical values of the reaction rate constants ksub(a) and ksub(d) were determined as well as equilibrium constants K. By means of the empirical formulae, the approximate incubation time for the RIA kit and maximal percent of insulin-/sup 125/I binding to antibody (%B) in relation to temperature were computed. The proposed method may be applied to the new antigen-binder systems preparation (new antibodies, shortening of the incubation time, temperature changes, influence of different ions and kind of buffer). (orig.) 891 MG/orig. 892 MBE.}
journal = []
volume = {4:6}
journal type = {AC}
place = {Germany}
year = {1979}
month = {Dec}
}