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Detection of diffusible substances

Abstract

The different steps of a radioautographic technique for the detection of diffusible substances are described. Using this radioautographic method, the topographic distribution of estradiol-concentrating neurons was studied in the nervous system and pituitary of the ovariectomized mouse and guinea-pig. A relatively good morphological preservation of structures can be ascertained on sections from unfixed, unembedded tissues prepared at low temperatures and kept-under relatively low humidity. The translocation or extraction of diffusible substances is avoided by directly mounting of frozen sections on dried photographic emulsion. Since no solvent is used, this technique excludes the major sources of diffusion artifacts and permits to be in favourable conditions for the localization of diffusible substances.
Authors:
Warembourg, M [1] 
  1. Lille-1 Univ., 59 - Villeneuve-d'Ascq (France)
Publication Date:
Dec 01, 1976
Product Type:
Conference
Reference Number:
AIX-08-330502; EDB-78-028464
Resource Relation:
Journal Name: J. Microsc. Biol. Cell.; (France); Journal Volume: 27:2; Conference: Meeting on techniques in radioautography, Paris, France, 26 Sep - 7 Oct 1975
Subject:
59 BASIC BIOLOGICAL SCIENCES; ESTRADIOL; BIOLOGICAL LOCALIZATION; NERVE CELLS; HISTOLOGICAL TECHNIQUES; AUTORADIOGRAPHY; ANIMAL CELLS; ESTRANES; ESTROGENS; HORMONES; HYDROXY COMPOUNDS; ORGANIC COMPOUNDS; SOMATIC CELLS; STEROID HORMONES; STEROIDS; 550301* - Cytology- Tracer Techniques
OSTI ID:
5333023
Country of Origin:
France
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JMBCD
Submitting Site:
INIS
Size:
Pages: . 277-280
Announcement Date:

Citation Formats

Warembourg, M. Detection of diffusible substances. France: N. p., 1976. Web.
Warembourg, M. Detection of diffusible substances. France.
Warembourg, M. 1976. "Detection of diffusible substances." France.
@misc{etde_5333023,
title = {Detection of diffusible substances}
author = {Warembourg, M}
abstractNote = {The different steps of a radioautographic technique for the detection of diffusible substances are described. Using this radioautographic method, the topographic distribution of estradiol-concentrating neurons was studied in the nervous system and pituitary of the ovariectomized mouse and guinea-pig. A relatively good morphological preservation of structures can be ascertained on sections from unfixed, unembedded tissues prepared at low temperatures and kept-under relatively low humidity. The translocation or extraction of diffusible substances is avoided by directly mounting of frozen sections on dried photographic emulsion. Since no solvent is used, this technique excludes the major sources of diffusion artifacts and permits to be in favourable conditions for the localization of diffusible substances.}
journal = {J. Microsc. Biol. Cell.; (France)}
volume = {27:2}
place = {France}
year = {1976}
month = {Dec}
}