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Qualitative determination of tubulin by radioimmunoassay

Journal Article:

Abstract

The use of an antiserum specific for tubulin in cytochemical studies was described previously. Here a report is presented on its application in a radioimmunoassay for tubulin useful in the concentration range of 0.5 to 20 ..mu..g tubulin/ml. The advantages of this technique over the classical colchicine binding assay are discussed in terms of sensitivity and nonsusceptibility to the degree of polymerization and thermal denaturation of tubulin allowing its application to cell culture homogenates.
Authors:
Joniau, M; [1]  Brabander, M de; [2]  Mey, J de; [3]  Hoebeke, J [4] 
  1. Louvain Univ. (Belgium). Interdisciplinary Research Centre
  2. Janssen Pharmaceutica, Beerse (Belgium). Lab. of Oncology
  3. Brussels Univ. (Belgium). Medische Stichting Koningin Elisabeth
  4. Brussels Univ. (Belgium). Lab. of Biochemical Pathology
Publication Date:
Jun 15, 1977
Product Type:
Journal Article
Reference Number:
AIX-08-330544; EDB-78-028482
Resource Relation:
Journal Name: FEBS (Fed. Eur. Biochem. Soc.) Lett.; (Netherlands); Journal Volume: 78:2
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; PROTEINS; RADIOIMMUNOASSAY; ANTIBODIES; BRAIN; CHEMICAL BONDS; COATINGS; COLCHICINE; COMPARATIVE EVALUATIONS; IODINE 125; LABELLED COMPOUNDS; RATS; TRITIUM COMPOUNDS; TUBES; ALKALOIDS; ANIMALS; ANTIMITOTIC DRUGS; BETA DECAY RADIOISOTOPES; BODY; CENTRAL NERVOUS SYSTEM; DAYS LIVING RADIOISOTOPES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; NERVOUS SYSTEM; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; RADIOACTIVE MATERIALS; RADIOASSAY; RADIOISOTOPES; RODENTS; TRACER TECHNIQUES; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5332896
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: FEBLA
Submitting Site:
INIS
Size:
Pages: 307-312
Announcement Date:
Dec 01, 1977

Journal Article:

Citation Formats

Joniau, M, Brabander, M de, Mey, J de, and Hoebeke, J. Qualitative determination of tubulin by radioimmunoassay. Netherlands: N. p., 1977. Web.
Joniau, M, Brabander, M de, Mey, J de, & Hoebeke, J. Qualitative determination of tubulin by radioimmunoassay. Netherlands.
Joniau, M, Brabander, M de, Mey, J de, and Hoebeke, J. 1977. "Qualitative determination of tubulin by radioimmunoassay." Netherlands.
@misc{etde_5332896,
title = {Qualitative determination of tubulin by radioimmunoassay}
author = {Joniau, M, Brabander, M de, Mey, J de, and Hoebeke, J}
abstractNote = {The use of an antiserum specific for tubulin in cytochemical studies was described previously. Here a report is presented on its application in a radioimmunoassay for tubulin useful in the concentration range of 0.5 to 20 ..mu..g tubulin/ml. The advantages of this technique over the classical colchicine binding assay are discussed in terms of sensitivity and nonsusceptibility to the degree of polymerization and thermal denaturation of tubulin allowing its application to cell culture homogenates.}
journal = {FEBS (Fed. Eur. Biochem. Soc.) Lett.; (Netherlands)}
volume = {78:2}
journal type = {AC}
place = {Netherlands}
year = {1977}
month = {Jun}
}