The use of an antiserum specific for tubulin in cytochemical studies was described previously. Here a report is presented on its application in a radioimmunoassay for tubulin useful in the concentration range of 0.5 to 20 ..mu..g tubulin/ml. The advantages of this technique over the classical colchicine binding assay are discussed in terms of sensitivity and nonsusceptibility to the degree of polymerization and thermal denaturation of tubulin allowing its application to cell culture homogenates.
Joniau, M;  Brabander, M de;  Mey, J de;  Hoebeke, J 
- Louvain Univ. (Belgium). Interdisciplinary Research Centre
- Janssen Pharmaceutica, Beerse (Belgium). Lab. of Oncology
- Brussels Univ. (Belgium). Medische Stichting Koningin Elisabeth
- Brussels Univ. (Belgium). Lab. of Biochemical Pathology