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X-ray induced DNA double strand break production and repair in mammalian cells as measured by neutral filter elution

Abstract

A neutral filter elution method was used for detecting DNA double strand breaks in mouse L1210 cells after X-ray. The assay detected the number of double strand breaks induced by as little as 1000 rad of X-ray. The rate of DNA elution through the filters under neutral conditions increased with X-ray dose. Certain conditions for deproteinization, pH, and filter type were shown to increase the assay's sensitivity. Hydrogen peroxide and Bleomycin also induced apparent DNA double strand breaks, although the ratios of double to single strand breaks varied from those produced by X-ray. The introduction of double strand cuts by HpA I restriction endonuclease in DNA lysed on filters resulted in a rapid rate of elution under neutral conditions, implying that the method can detect double strand breaks if they exist in the DNA. The eluted DNA banded with a double stranded DNA marker in cesium chloride. This evidence suggested that the assay detected DNA double strand breaks. L1210 cells were shown to rejoin most of the DNA double strand breaks induced by 5-10 krad of X-ray with a half-time of about 40 minutes. (author).
Authors:
Bradley, M O; Kohn, K W [1] 
  1. National Institutes of Health, Bethesda, MD (USA)
Publication Date:
Oct 01, 1979
Product Type:
Journal Article
Reference Number:
AIX-11-505034; EDB-80-073044
Resource Relation:
Journal Name: Nucleic Acids Res.; (United Kingdom); Journal Volume: 7:3
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; STRAND BREAKS; BIOASSAY; ANIMAL CELLS; BIOLOGICAL REPAIR; BLEOMYCIN; CARBON 14 COMPOUNDS; CELL CULTURES; DNA; DOSE-RESPONSE RELATIONSHIPS; EARLY RADIATION EFFECTS; EXTERNAL IRRADIATION; FILTRATION; HYDROGEN PEROXIDE; LABELLED COMPOUNDS; MICE; NUCLEASES; TRITIUM COMPOUNDS; X RADIATION; ANIMALS; ANTIBIOTICS; ANTIMITOTIC DRUGS; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL RECOVERY; DRUGS; ELECTROMAGNETIC RADIATION; ENZYMES; HYDROGEN COMPOUNDS; IONIZING RADIATIONS; IRRADIATION; MAMMALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; PEROXIDES; PHOSPHOTRANSFERASES; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; RODENTS; SEPARATION PROCESSES; TRANSFERASES; VERTEBRATES; 560121* - Radiation Effects on Cells- External Source- (-1987); 560301 - Chemicals Metabolism & Toxicology- Cells- (-1987)
OSTI ID:
5323116
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: NARHA
Submitting Site:
INIS
Size:
Pages: 793-804
Announcement Date:

Citation Formats

Bradley, M O, and Kohn, K W. X-ray induced DNA double strand break production and repair in mammalian cells as measured by neutral filter elution. United Kingdom: N. p., 1979. Web. doi:10.1093/nar/7.3.793.
Bradley, M O, & Kohn, K W. X-ray induced DNA double strand break production and repair in mammalian cells as measured by neutral filter elution. United Kingdom. doi:10.1093/nar/7.3.793.
Bradley, M O, and Kohn, K W. 1979. "X-ray induced DNA double strand break production and repair in mammalian cells as measured by neutral filter elution." United Kingdom. doi:10.1093/nar/7.3.793. https://www.osti.gov/servlets/purl/10.1093/nar/7.3.793.
@misc{etde_5323116,
title = {X-ray induced DNA double strand break production and repair in mammalian cells as measured by neutral filter elution}
author = {Bradley, M O, and Kohn, K W}
abstractNote = {A neutral filter elution method was used for detecting DNA double strand breaks in mouse L1210 cells after X-ray. The assay detected the number of double strand breaks induced by as little as 1000 rad of X-ray. The rate of DNA elution through the filters under neutral conditions increased with X-ray dose. Certain conditions for deproteinization, pH, and filter type were shown to increase the assay's sensitivity. Hydrogen peroxide and Bleomycin also induced apparent DNA double strand breaks, although the ratios of double to single strand breaks varied from those produced by X-ray. The introduction of double strand cuts by HpA I restriction endonuclease in DNA lysed on filters resulted in a rapid rate of elution under neutral conditions, implying that the method can detect double strand breaks if they exist in the DNA. The eluted DNA banded with a double stranded DNA marker in cesium chloride. This evidence suggested that the assay detected DNA double strand breaks. L1210 cells were shown to rejoin most of the DNA double strand breaks induced by 5-10 krad of X-ray with a half-time of about 40 minutes. (author).}
doi = {10.1093/nar/7.3.793}
journal = {Nucleic Acids Res.; (United Kingdom)}
volume = {7:3}
journal type = {AC}
place = {United Kingdom}
year = {1979}
month = {Oct}
}