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A sensitive assay for Staphylococcus aureus nucleases

Journal Article:

Abstract

A sensitive assay for staphylococcal nuclease involving incubation of the enzyme sample with heat-denatured ({sup 3}H) thymidine labelled DNA from E.coli, precipitation with trichloroacetic acid and measurement of the radioactivity of acid-soluble nucleotides released has been developed. The assay is sensitive enough to be used for comparing the levels of nucleases elaborated by different strains of S. aureus as well as for determining the extent of contamination of S. aureus in food and water samples even at levels at which the conventional spectrophotometric and toluidine blue-DNA methods are totally inadequate. (author). 26 refs., 3 figs ., 3 tabs.
Authors:
Kohli, J K; Vakil, B V; Patil, M S; Pandey, V N; Pradhan, D S [1] 
  1. Bhabha Atomic Reserach Centre, Bombay (India). Biochemistry Div.
Publication Date:
Oct 01, 1989
Product Type:
Journal Article
Reference Number:
AIX-22-072714; EDB-91-135777
Resource Relation:
Journal Name: Indian Journal of Biochemistry and Biophysics; (India); Journal Volume: 26:5
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; NUCLEASES; RADIOENZYMATIC ASSAY; STAPHYLOCOCCUS; BIOASSAY; ENZYME ACTIVITY; FOOD; LIQUID SCINTILLATION DETECTORS; SENSITIVITY; THYMIDINE; TRACER TECHNIQUES; TRITIUM COMPOUNDS; WHOLESOMENESS; AZINES; BACTERIA; ENZYMES; ESTERASES; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; HYDROLASES; ISOTOPE APPLICATIONS; MEASURING INSTRUMENTS; MICROORGANISMS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PHOSPHODIESTERASES; PYRIMIDINES; RADIATION DETECTORS; RIBOSIDES; SCINTILLATION COUNTERS; 550201* - Biochemistry- Tracer Techniques; 553004 - Agriculture & Food Technology- Food Protection & Preservation- (1987-)
OSTI ID:
5316890
Country of Origin:
India
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0301-1208; CODEN: IJBBB
Submitting Site:
INIS
Size:
Pages: 296-300
Announcement Date:

Journal Article:

Citation Formats

Kohli, J K, Vakil, B V, Patil, M S, Pandey, V N, and Pradhan, D S. A sensitive assay for Staphylococcus aureus nucleases. India: N. p., 1989. Web.
Kohli, J K, Vakil, B V, Patil, M S, Pandey, V N, & Pradhan, D S. A sensitive assay for Staphylococcus aureus nucleases. India.
Kohli, J K, Vakil, B V, Patil, M S, Pandey, V N, and Pradhan, D S. 1989. "A sensitive assay for Staphylococcus aureus nucleases." India.
@misc{etde_5316890,
title = {A sensitive assay for Staphylococcus aureus nucleases}
author = {Kohli, J K, Vakil, B V, Patil, M S, Pandey, V N, and Pradhan, D S}
abstractNote = {A sensitive assay for staphylococcal nuclease involving incubation of the enzyme sample with heat-denatured ({sup 3}H) thymidine labelled DNA from E.coli, precipitation with trichloroacetic acid and measurement of the radioactivity of acid-soluble nucleotides released has been developed. The assay is sensitive enough to be used for comparing the levels of nucleases elaborated by different strains of S. aureus as well as for determining the extent of contamination of S. aureus in food and water samples even at levels at which the conventional spectrophotometric and toluidine blue-DNA methods are totally inadequate. (author). 26 refs., 3 figs ., 3 tabs.}
journal = {Indian Journal of Biochemistry and Biophysics; (India)}
volume = {26:5}
journal type = {AC}
place = {India}
year = {1989}
month = {Oct}
}