You need JavaScript to view this

Rapid detection of microalbuminuria in diabetic patients by an agglutination inhibition test. Comparison with radioimmunoassay

Journal Article:

Abstract

Subclinical elevation of urinary albumin excretion (UAlbE) early in the course of diabetes mellitus has been suggested to predict later clinical proteinuria and mortality. UAlbE is currently measured using radioimmunoassay (RIA) or radial immunodiffusion methods. However, these procedures are expensive and time-consuming and cannot be used as screening methods. Recently, an agglutination test (AT) has been suggested as a routinary method for the screening of microalbuminuria in diabetic patients. In this paper the results obtained are compared with an AT procedure and RIA method in a screening program of microproteinuria in diabetic patients. An immunological test (a latex agglutination assay) for the analysis of albuminura is used, which human albumin was adsorbed to latex beads (about 0.3 ..mu..l of a urine sample. Urine samples containing an albumin concentration >40 ..mu..g/ml were found to inhibit the agglutination of latex beads with antiserum. The RIA and AT results showed good agreement when urine samples were assayed soon after collection or after a short period of storage (less than or equal to3 weeks at -20 grade centigrades). The AT procedure has been adjusted in order to give a positive response (no agglutination) over the range of supranormal concentrations of urinary albumin (>40 ..mu..g/ml),  More>>
Publication Date:
Jan 01, 1986
Product Type:
Journal Article
Reference Number:
AIX-19-045258; EDB-88-080730
Resource Relation:
Journal Name: J. Nucl. Med. Allied Sci.; (Italy); Journal Volume: 30:4
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ANTIGEN-ANTIBODY REACTIONS; ALBUMINURIA; RADIOIMMUNOASSAY; ALBUMINS; COMPARATIVE EVALUATIONS; DIABETES MELLITUS; DISEASES; ENDOCRINE DISEASES; IMMUNOASSAY; IMMUNOLOGY; ISOTOPE APPLICATIONS; METABOLIC DISEASES; ORGANIC COMPOUNDS; PROTEINS; RADIOASSAY; RADIOIMMUNOLOGY; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5315280
Research Organizations:
Pisa Univ. (Italy). Cattedra di Malattie del Ricambio, Ist. di Clinica Medica II; Consiglio Nazionale delle Ricerche, Pisa (Italy). Ist. di Clinica Fisiologica
Country of Origin:
Italy
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JNMSD
Submitting Site:
INIS
Size:
Pages: 215-219
Announcement Date:

Journal Article:

Citation Formats

Giampietro, O, Miccoli, R, Di Palma, L, Bertolotto, A, Anichini, R, Navalesi, R, and Clerico, A. Rapid detection of microalbuminuria in diabetic patients by an agglutination inhibition test. Comparison with radioimmunoassay. Italy: N. p., 1986. Web.
Giampietro, O, Miccoli, R, Di Palma, L, Bertolotto, A, Anichini, R, Navalesi, R, & Clerico, A. Rapid detection of microalbuminuria in diabetic patients by an agglutination inhibition test. Comparison with radioimmunoassay. Italy.
Giampietro, O, Miccoli, R, Di Palma, L, Bertolotto, A, Anichini, R, Navalesi, R, and Clerico, A. 1986. "Rapid detection of microalbuminuria in diabetic patients by an agglutination inhibition test. Comparison with radioimmunoassay." Italy.
@misc{etde_5315280,
title = {Rapid detection of microalbuminuria in diabetic patients by an agglutination inhibition test. Comparison with radioimmunoassay}
author = {Giampietro, O, Miccoli, R, Di Palma, L, Bertolotto, A, Anichini, R, Navalesi, R, and Clerico, A}
abstractNote = {Subclinical elevation of urinary albumin excretion (UAlbE) early in the course of diabetes mellitus has been suggested to predict later clinical proteinuria and mortality. UAlbE is currently measured using radioimmunoassay (RIA) or radial immunodiffusion methods. However, these procedures are expensive and time-consuming and cannot be used as screening methods. Recently, an agglutination test (AT) has been suggested as a routinary method for the screening of microalbuminuria in diabetic patients. In this paper the results obtained are compared with an AT procedure and RIA method in a screening program of microproteinuria in diabetic patients. An immunological test (a latex agglutination assay) for the analysis of albuminura is used, which human albumin was adsorbed to latex beads (about 0.3 ..mu..l of a urine sample. Urine samples containing an albumin concentration >40 ..mu..g/ml were found to inhibit the agglutination of latex beads with antiserum. The RIA and AT results showed good agreement when urine samples were assayed soon after collection or after a short period of storage (less than or equal to3 weeks at -20 grade centigrades). The AT procedure has been adjusted in order to give a positive response (no agglutination) over the range of supranormal concentrations of urinary albumin (>40 ..mu..g/ml), which are on the other hand undetectable by Albustix. In addition, it is possible to perform a semiquantitative test using various dilutions of urine samples with albumin concentration > 40 ..mu..g/ml, so to estimate approximately the UAlbE. The AT method is simple, fast and specific, and has proved to be useful for the identification of diabetic patients at risk for developing clinical nephropathy. Therefore, it may be used in screening programs for diabetic microproteinuria. 21 refs.}
journal = {J. Nucl. Med. Allied Sci.; (Italy)}
volume = {30:4}
journal type = {AC}
place = {Italy}
year = {1986}
month = {Jan}
}