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Saturation of DNA repair in mammalian cells

Journal Article:

Abstract

Excision repair seems to reach a plateau in normal human cells at a 254 nm dose near 20 J/m/sup 2/. We measured excision repair in normal human fibroblasts up to 80 J/m/sup 2/. The four techniques used (unscheduled DNA synthesis, photolysis of BrdUrd incorporated during repair, loss of sites sensitive to a UV endonuclease from Micrococcus luteus, and loss of pyrimidine dimers from DNA) showed little difference between the two doses. Moreover, the loss of endonuclease sites in 24h following two 20 J/m/sup 2/ doses separated by 24h was similar to the loss observed following one dose. Hence, we concluded that the observed plateau in excision repair is real and does not represent some inhibitory process at high doses but a true saturation of one of the rate limiting steps in repair.
Publication Date:
Jan 01, 1979
Product Type:
Journal Article
Reference Number:
ERA-05-029658; EDB-80-085858
Resource Relation:
Journal Name: Photochem. Photobiol.; (United Kingdom); Journal Volume: 29
Subject:
59 BASIC BIOLOGICAL SCIENCES; BIOLOGICAL REPAIR; BIOCHEMICAL REACTION KINETICS; DNA; BIOLOGICAL EFFECTS; FIBROBLASTS; BIOSYNTHESIS; CELL CULTURES; DIMERS; DNA-ASE; DOSE-RESPONSE RELATIONSHIPS; MAN; PHOTOLYSIS; PYRIMIDINES; ANIMAL CELLS; ANIMALS; AZINES; BIOLOGICAL RECOVERY; CHEMICAL REACTIONS; CONNECTIVE TISSUE CELLS; DECOMPOSITION; ENZYMES; ESTERASES; HETEROCYCLIC COMPOUNDS; HYDROLASES; KINETICS; MAMMALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PHOSPHATASES; PHOTOCHEMICAL REACTIONS; PRIMATES; REACTION KINETICS; RECOVERY; REPAIR; SOMATIC CELLS; SYNTHESIS; VERTEBRATES; 550200* - Biochemistry; 551000 - Physiological Systems
OSTI ID:
5308066
Research Organizations:
Brookhaven National Lab., Upton, NY
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: PHCBA
Submitting Site:
BNL
Size:
Pages: 983-989
Announcement Date:
Aug 01, 1980

Journal Article:

Citation Formats

Ahmed, F E, and Setlow, R B. Saturation of DNA repair in mammalian cells. United Kingdom: N. p., 1979. Web.
Ahmed, F E, & Setlow, R B. Saturation of DNA repair in mammalian cells. United Kingdom.
Ahmed, F E, and Setlow, R B. 1979. "Saturation of DNA repair in mammalian cells." United Kingdom.
@misc{etde_5308066,
title = {Saturation of DNA repair in mammalian cells}
author = {Ahmed, F E, and Setlow, R B}
abstractNote = {Excision repair seems to reach a plateau in normal human cells at a 254 nm dose near 20 J/m/sup 2/. We measured excision repair in normal human fibroblasts up to 80 J/m/sup 2/. The four techniques used (unscheduled DNA synthesis, photolysis of BrdUrd incorporated during repair, loss of sites sensitive to a UV endonuclease from Micrococcus luteus, and loss of pyrimidine dimers from DNA) showed little difference between the two doses. Moreover, the loss of endonuclease sites in 24h following two 20 J/m/sup 2/ doses separated by 24h was similar to the loss observed following one dose. Hence, we concluded that the observed plateau in excision repair is real and does not represent some inhibitory process at high doses but a true saturation of one of the rate limiting steps in repair.}
journal = {Photochem. Photobiol.; (United Kingdom)}
volume = {29}
journal type = {AC}
place = {United Kingdom}
year = {1979}
month = {Jan}
}