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Photoreactivation of thymine dimers in uv-irradiated human cells: unique dependence on culture conditions

Journal Article:

Abstract

UV-irradiated human fibroblasts in tissue culture were exposed to photoreactivating light in an attempt to demonstrate a light-dependent loss of thymine dimers from the acid-insoluble fraction of the DNA. The only experimental conditions in which this phenomenon was observed was if the cells were grown for at least 10 days in Dulbecco's modified Eagle's minimum essential medium. Such cells lost a maximum of between 10 to 30% of the thymine dimers from their DNA during illumination for 1 h. When cells were grown in a variety of other media, this phenomenon was not observed. The present experiments do not discriminate between true enzymatic photoreactivation and a medium-dependent photosensitization phenomenon that is not enzymatic in nature.
Authors:
Mortelmans, K; Friedberg, E C; [1]  Cleaver, J E; Thomas, G H; [2]  Paterson, M C; Smith, B P [3] 
  1. Stanford Univ., Calif. (USA). Dept. of Pathology. Lab. of Experimental Oncology
  2. California Univ., San Francisco (USA). Lab. of Radiobiology
  3. Atomic Energy of Canada Ltd., Chalk River, Ontario. Biology and Health Physics Div. Chalk River Nuclear Labs.
Publication Date:
Sep 01, 1977
Product Type:
Journal Article
Reference Number:
AIX-08-346820; EDB-78-046769
Resource Relation:
Journal Name: Mutat. Res.; (Netherlands); Journal Volume: 44:3
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DIMERS; PHOTOREACTIVATION; CELL CULTURES; COMPARATIVE EVALUATIONS; CULTURE MEDIA; DNA; FIBROBLASTS; IRRADIATION; LABELLED COMPOUNDS; MAN; THIN-LAYER CHROMATOGRAPHY; THYMINE; TRITIUM COMPOUNDS; ULTRAVIOLET RADIATION; ANIMAL CELLS; ANIMALS; AZINES; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; CHROMATOGRAPHY; CONNECTIVE TISSUE CELLS; ELECTROMAGNETIC RADIATION; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; MAMMALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PRIMATES; PYRIMIDINES; RADIATIONS; RADIOACTIVE MATERIALS; RECOVERY; REPAIR; SEPARATION PROCESSES; SOMATIC CELLS; URACILS; VERTEBRATES; 560121* - Radiation Effects on Cells- External Source- (-1987)
OSTI ID:
5302817
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: MUREA
Submitting Site:
INIS
Size:
Pages: 433-445
Announcement Date:

Journal Article:

Citation Formats

Mortelmans, K, Friedberg, E C, Cleaver, J E, Thomas, G H, Paterson, M C, and Smith, B P. Photoreactivation of thymine dimers in uv-irradiated human cells: unique dependence on culture conditions. Netherlands: N. p., 1977. Web.
Mortelmans, K, Friedberg, E C, Cleaver, J E, Thomas, G H, Paterson, M C, & Smith, B P. Photoreactivation of thymine dimers in uv-irradiated human cells: unique dependence on culture conditions. Netherlands.
Mortelmans, K, Friedberg, E C, Cleaver, J E, Thomas, G H, Paterson, M C, and Smith, B P. 1977. "Photoreactivation of thymine dimers in uv-irradiated human cells: unique dependence on culture conditions." Netherlands.
@misc{etde_5302817,
title = {Photoreactivation of thymine dimers in uv-irradiated human cells: unique dependence on culture conditions}
author = {Mortelmans, K, Friedberg, E C, Cleaver, J E, Thomas, G H, Paterson, M C, and Smith, B P}
abstractNote = {UV-irradiated human fibroblasts in tissue culture were exposed to photoreactivating light in an attempt to demonstrate a light-dependent loss of thymine dimers from the acid-insoluble fraction of the DNA. The only experimental conditions in which this phenomenon was observed was if the cells were grown for at least 10 days in Dulbecco's modified Eagle's minimum essential medium. Such cells lost a maximum of between 10 to 30% of the thymine dimers from their DNA during illumination for 1 h. When cells were grown in a variety of other media, this phenomenon was not observed. The present experiments do not discriminate between true enzymatic photoreactivation and a medium-dependent photosensitization phenomenon that is not enzymatic in nature.}
journal = {Mutat. Res.; (Netherlands)}
volume = {44:3}
journal type = {AC}
place = {Netherlands}
year = {1977}
month = {Sep}
}