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Radioimmunoassay for human ACTH

Journal Article:

Abstract

A radioimmunoassay method for human adrenocorticotropic hormone (ACTH) is described. The antiserum was produced in a guinea pig by multiple injections of a total of 1 mg of porcine ACTH adsorbed with CM-cellulose and mixed with complete Freund's adjuvant. The antiserum used for this study at a final dilution of 1:500,000 was obtained from a guinea pig after 10 immunizations. A highly purified native ACTH (Li, ..cap alpha.. sub(h)sup(1-39) ACTH) was used as an assay standard and a tracer hormone. Separation of free and bound hormone was achieved by dextran coated charcoal. Extraction of ACTH from plasma samples was performed by Donald's method modified with silicic acid. The antibody appeared to be directed against the C-terminal portion of the hormone molecule and showed no significant reaction with other pituitary hormones (GH, TSH, LH, FSH, Hpr, Oxytocin, Arginine-and Lysine-Vasopressin). Plasma ACTH levels of 5 healthy subjects at nine o'clock averaged 32 +- 8.5 pg/ml (M +- SD). Plasma ACTH concentrations in a case of Sheehan's syndrome and of an untreated adrenogenital syndrome at nine o'clock were less than 20 and 194 pg/ml, respectively. A case of Cushing's syndrome accompanied with bilateral nodular hyperplasia of the adrenal cortex showed diminished diurnal variation  More>>
Authors:
Matsumura, S; Oyama, H; Tenku, A; Horino, M; [1]  Kobayashi, K
  1. Kawasaki Medicial School, Kurashiki, Okayama (Japan)
Publication Date:
Mar 01, 1977
Product Type:
Journal Article
Reference Number:
AIX-09-354949; EDB-78-033991
Resource Relation:
Journal Name: Kazan; (Japan); Journal Volume: 2:3
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ACTH; RADIOIMMUNOASSAY; BLOOD PLASMA; IMMUNE SERUMS; IODINE 125; RADIOPHARMACEUTICALS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BODY FLUIDS; DAYS LIVING RADIOISOTOPES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LABELLED COMPOUNDS; NUCLEI; ODD-EVEN NUCLEI; PEPTIDE HORMONES; PITUITARY HORMONES; RADIOACTIVE MATERIALS; RADIOASSAY; RADIOISOTOPES; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5300936
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Journal ID: CODEN: KAZAA
Submitting Site:
INIS
Size:
Pages: . 131-136
Announcement Date:

Journal Article:

Citation Formats

Matsumura, S, Oyama, H, Tenku, A, Horino, M, and Kobayashi, K. Radioimmunoassay for human ACTH. Japan: N. p., 1977. Web.
Matsumura, S, Oyama, H, Tenku, A, Horino, M, & Kobayashi, K. Radioimmunoassay for human ACTH. Japan.
Matsumura, S, Oyama, H, Tenku, A, Horino, M, and Kobayashi, K. 1977. "Radioimmunoassay for human ACTH." Japan.
@misc{etde_5300936,
title = {Radioimmunoassay for human ACTH}
author = {Matsumura, S, Oyama, H, Tenku, A, Horino, M, and Kobayashi, K}
abstractNote = {A radioimmunoassay method for human adrenocorticotropic hormone (ACTH) is described. The antiserum was produced in a guinea pig by multiple injections of a total of 1 mg of porcine ACTH adsorbed with CM-cellulose and mixed with complete Freund's adjuvant. The antiserum used for this study at a final dilution of 1:500,000 was obtained from a guinea pig after 10 immunizations. A highly purified native ACTH (Li, ..cap alpha.. sub(h)sup(1-39) ACTH) was used as an assay standard and a tracer hormone. Separation of free and bound hormone was achieved by dextran coated charcoal. Extraction of ACTH from plasma samples was performed by Donald's method modified with silicic acid. The antibody appeared to be directed against the C-terminal portion of the hormone molecule and showed no significant reaction with other pituitary hormones (GH, TSH, LH, FSH, Hpr, Oxytocin, Arginine-and Lysine-Vasopressin). Plasma ACTH levels of 5 healthy subjects at nine o'clock averaged 32 +- 8.5 pg/ml (M +- SD). Plasma ACTH concentrations in a case of Sheehan's syndrome and of an untreated adrenogenital syndrome at nine o'clock were less than 20 and 194 pg/ml, respectively. A case of Cushing's syndrome accompanied with bilateral nodular hyperplasia of the adrenal cortex showed diminished diurnal variation and normal levels of plasma ACTH. In contrast, elevated plasma ACTH levels and lack of diurnal rhythm of ACTH secretion were observed in a suspected case of Cushing's disease.}
journal = {Kazan; (Japan)}
volume = {2:3}
journal type = {AC}
place = {Japan}
year = {1977}
month = {Mar}
}