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Properties of an endonuclease activity in Micrococcus luteus acting on. gamma. -irradiated DNA and on apurinic DNA

Journal Article:

Abstract

A protein fraction from Micrococcus luteus with endonuclease activity against ..gamma..-irradiated DNA was isolated and characterized. An additional activity on apurinic sites could not be separated, either by sucrose gradient sedimentation or by gel filtration through Sephadex G 100. From gel filtration, a molecular weight of about 25 000 was calculated for both endonuclease activities. The endonuclease activity against ..gamma..-irradiated DNA was stimulated five-fold with 5 mM Mg/sup + +/, whereas that against apurinic sites was less dependent on the Mg/sup + +/ concentration. 100 mM KCl inhibited the ..gamma..-ray endonuclease, but not the apurinic endonuclease activity. In ..gamma..-irradiated DNA the protein recognized 1.65 endonuclease sensitive sites per radiation-induced single-strand break, among which are 0.45 alkali labile lesions in the nucleotide strand. The was evaluated resulting in a Ksub(m)-value of 73 nM.
Authors:
Schaefer, G; Haas, P; Coquerelle, Th; Hagen, U [1] 
  1. Kernforschungszentrum Karlsruhe G.m.b.H. (Germany, F.R.). Inst. fuer Genetik und fuer Toxikologie von Spaltstoffen
Publication Date:
Jan 01, 1980
Product Type:
Journal Article
Reference Number:
AIX-11-527747; EDB-80-086367
Resource Relation:
Journal Name: Int. J. Radiat. Biol.; (United Kingdom); Journal Volume: 37:1
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; MICROCOCCUS LUTEUS; NUCLEASES; ENZYME ACTIVITY; SPECIFICITY; BIOCHEMICAL REACTION KINETICS; CATALYSIS; CHEMICAL RADIATION EFFECTS; DNA; GAMMA RADIATION; MAGNESIUM COMPOUNDS; METABOLISM; MOLECULAR WEIGHT; NUCLEOPROTEINS; QUANTITY RATIO; STIMULATION; STRAND BREAKS; ALKALINE EARTH METAL COMPOUNDS; BACTERIA; CHEMISTRY; ELECTROMAGNETIC RADIATION; ENZYMES; IONIZING RADIATIONS; KINETICS; MICROCOCCUS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PHOSPHOTRANSFERASES; PROTEINS; RADIATION CHEMISTRY; RADIATION EFFECTS; RADIATIONS; REACTION KINETICS; TRANSFERASES; 560111* - Radiation Effects on Biochemicals- In Vitro- (-1987)
OSTI ID:
5299375
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: IJRBA
Submitting Site:
INIS
Size:
Pages: 11-18
Announcement Date:

Journal Article:

Citation Formats

Schaefer, G, Haas, P, Coquerelle, Th, and Hagen, U. Properties of an endonuclease activity in Micrococcus luteus acting on. gamma. -irradiated DNA and on apurinic DNA. United Kingdom: N. p., 1980. Web.
Schaefer, G, Haas, P, Coquerelle, Th, & Hagen, U. Properties of an endonuclease activity in Micrococcus luteus acting on. gamma. -irradiated DNA and on apurinic DNA. United Kingdom.
Schaefer, G, Haas, P, Coquerelle, Th, and Hagen, U. 1980. "Properties of an endonuclease activity in Micrococcus luteus acting on. gamma. -irradiated DNA and on apurinic DNA." United Kingdom.
@misc{etde_5299375,
title = {Properties of an endonuclease activity in Micrococcus luteus acting on. gamma. -irradiated DNA and on apurinic DNA}
author = {Schaefer, G, Haas, P, Coquerelle, Th, and Hagen, U}
abstractNote = {A protein fraction from Micrococcus luteus with endonuclease activity against ..gamma..-irradiated DNA was isolated and characterized. An additional activity on apurinic sites could not be separated, either by sucrose gradient sedimentation or by gel filtration through Sephadex G 100. From gel filtration, a molecular weight of about 25 000 was calculated for both endonuclease activities. The endonuclease activity against ..gamma..-irradiated DNA was stimulated five-fold with 5 mM Mg/sup + +/, whereas that against apurinic sites was less dependent on the Mg/sup + +/ concentration. 100 mM KCl inhibited the ..gamma..-ray endonuclease, but not the apurinic endonuclease activity. In ..gamma..-irradiated DNA the protein recognized 1.65 endonuclease sensitive sites per radiation-induced single-strand break, among which are 0.45 alkali labile lesions in the nucleotide strand. The was evaluated resulting in a Ksub(m)-value of 73 nM.}
journal = {Int. J. Radiat. Biol.; (United Kingdom)}
volume = {37:1}
journal type = {AC}
place = {United Kingdom}
year = {1980}
month = {Jan}
}