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Effect of salt solutions on the radiosensitivity of mammalian cells as a function of the state of adhesion and the water structure

Abstract

The radiation isodose survival curve of attached Chinese hamster (V79) cells, subjected to a wide concentration range of salt or sucrose solutions, was characterized by two maxima separated by a minimum. Cells were radioprotected at the maxima (high and low hypertonic salt concentrations) while they were radiosensitized at the minimum (intermediate hypertonic salt concentrations). Both cations and anions could alter the cellular radiosensitivity above and beyond the (osmotic) effect observed for cells treated with sucrose solutions. However, the basic curve shape, except in the case of sulphate salts, remained the same. When these experiments were repeated with single cells in suspension, the isodose survival curve was quite different in that high salt concentrations did not protect cells in suspension unlike the case with attached cells. The curve shape was also altered in that the second maximum was absent with many salt solutions. When multicellular spheroids were used for these experiments, the data resembled those for single cell suspensions rather than for attached cells. The radiation survival data for cells in suspension in salt solutions correlated with water proton spin lattice relaxation time (T/sub 1/) and, in hypo- and iso-tonic solutions, with cell volume.
Authors:
Moggach, P G; Lepock, J R; Kruuv, J [1] 
  1. Waterloo Univ., Ontario (Canada). Dept. of Physics
Publication Date:
Nov 01, 1979
Product Type:
Journal Article
Reference Number:
AIX-11-510584; EDB-80-079769
Resource Relation:
Journal Name: Int. J. Radiat. Biol.; (United Kingdom); Journal Volume: 36:5
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CELL CULTURES; RADIOSENSITIVITY; POTASSIUM CHLORIDES; RADIOSENSITIVITY EFFECTS; SODIUM CHLORIDES; SODIUM SULFATES; ALKALI METAL COMPOUNDS; ALKALI METAL ISOTOPES; ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; CARBOHYDRATES; CESIUM ISOTOPES; CHLORIDES; CHLORINE COMPOUNDS; CONNECTIVE TISSUE CELLS; DISACCHARIDES; DISPERSIONS; ELECTROMAGNETIC RADIATION; HALIDES; HALOGEN COMPOUNDS; IONIZING RADIATIONS; IRRADIATION; ISOTOPES; MAMMALS; MIXTURES; NUCLEI; ODD-EVEN NUCLEI; OLIGOSACCHARIDES; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; POTASSIUM COMPOUNDS; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; RODENTS; SACCHARIDES; SODIUM COMPOUNDS; SOLUTIONS; SOMATIC CELLS; SULFATES; SULFUR COMPOUNDS; VERTEBRATES; YEARS LIVING RADIOISOTOPES; 560111* - Radiation Effects on Biochemicals- In Vitro- (-1987)
OSTI ID:
5289916
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: IJRBA
Submitting Site:
INIS
Size:
Pages: 435-451
Announcement Date:
May 01, 1980

Citation Formats

Moggach, P G, Lepock, J R, and Kruuv, J. Effect of salt solutions on the radiosensitivity of mammalian cells as a function of the state of adhesion and the water structure. United Kingdom: N. p., 1979. Web. doi:10.1080/09553007914551241.
Moggach, P G, Lepock, J R, & Kruuv, J. Effect of salt solutions on the radiosensitivity of mammalian cells as a function of the state of adhesion and the water structure. United Kingdom. doi:10.1080/09553007914551241.
Moggach, P G, Lepock, J R, and Kruuv, J. 1979. "Effect of salt solutions on the radiosensitivity of mammalian cells as a function of the state of adhesion and the water structure." United Kingdom. doi:10.1080/09553007914551241. https://www.osti.gov/servlets/purl/10.1080/09553007914551241.
@misc{etde_5289916,
title = {Effect of salt solutions on the radiosensitivity of mammalian cells as a function of the state of adhesion and the water structure}
author = {Moggach, P G, Lepock, J R, and Kruuv, J}
abstractNote = {The radiation isodose survival curve of attached Chinese hamster (V79) cells, subjected to a wide concentration range of salt or sucrose solutions, was characterized by two maxima separated by a minimum. Cells were radioprotected at the maxima (high and low hypertonic salt concentrations) while they were radiosensitized at the minimum (intermediate hypertonic salt concentrations). Both cations and anions could alter the cellular radiosensitivity above and beyond the (osmotic) effect observed for cells treated with sucrose solutions. However, the basic curve shape, except in the case of sulphate salts, remained the same. When these experiments were repeated with single cells in suspension, the isodose survival curve was quite different in that high salt concentrations did not protect cells in suspension unlike the case with attached cells. The curve shape was also altered in that the second maximum was absent with many salt solutions. When multicellular spheroids were used for these experiments, the data resembled those for single cell suspensions rather than for attached cells. The radiation survival data for cells in suspension in salt solutions correlated with water proton spin lattice relaxation time (T/sub 1/) and, in hypo- and iso-tonic solutions, with cell volume.}
doi = {10.1080/09553007914551241}
journal = {Int. J. Radiat. Biol.; (United Kingdom)}
volume = {36:5}
journal type = {AC}
place = {United Kingdom}
year = {1979}
month = {Nov}
}