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Studies on the production of insulin radio-immunoassay kit

Journal Article:

Abstract

Insulin was labelled with Iodine-125 in about 35% yield by applying the chloramine-T method. The specific activity of the labelled product was about 100 ..mu..Ci/ug. To use the labelled product for the radioimmunoassay of insulin, the well labelled fractions were selected through a starch gel electrophoresis autoradiography, elution, and subsequent incubations with insulin antibodies. The results of the standardizations using the well labelled insulin fractions for radioimmunoassay indicated that the ratio of the antibody bound (B) to the free (F) insulin-/sup 125/I is 0.2 to 1.6 in the standard insulin dose of up to 50 ..mu..U/ml, the relatively steep dose gradient. Kits were prepared and the stabilities were also checked.
Authors:
Kim, J R; Kim, T H; Kim, Y S [1] 
  1. Korea Atomic Energy Research Inst., Seoul (Republic of Korea)
Publication Date:
Jan 01, 1978
Product Type:
Journal Article
Reference Number:
AIX-11-521087; EDB-80-085040
Resource Relation:
Journal Name: Taehan Haekui Hakhoe Chapchi; (Korea, South); Journal Volume: 13:2
Subject:
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY; 62 RADIOLOGY AND NUCLEAR MEDICINE; INSULIN; LABELLING; IODINE 125; RADIOCHEMISTRY; RADIOIMMUNOASSAY; STANDARDIZATION; RADIOPHARMACEUTICALS; CHEMICAL PREPARATION; ANTIBODIES; AUTORADIOGRAPHY; CHEMICAL REACTION YIELD; CHLORAMINES; ELECTROPHORESIS; INCUBATION; IODINATION; AMINES; BETA DECAY RADIOISOTOPES; CHEMICAL REACTIONS; CHEMISTRY; DAYS LIVING RADIOISOTOPES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; HALOGENATION; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LABELLED COMPOUNDS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC CHLORINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; PEPTIDE HORMONES; RADIOACTIVE MATERIALS; RADIOASSAY; RADIOISOTOPES; SYNTHESIS; TRACER TECHNIQUES; YIELDS; 400702* - Radiochemistry & Nuclear Chemistry- Properties of Radioactive Materials; 550601 - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5269122
Country of Origin:
Korea, Republic of
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: THHCD
Submitting Site:
INIS
Size:
Pages: 27-33
Announcement Date:
Jun 01, 1980

Journal Article:

Citation Formats

Kim, J R, Kim, T H, and Kim, Y S. Studies on the production of insulin radio-immunoassay kit. Korea, Republic of: N. p., 1978. Web.
Kim, J R, Kim, T H, & Kim, Y S. Studies on the production of insulin radio-immunoassay kit. Korea, Republic of.
Kim, J R, Kim, T H, and Kim, Y S. 1978. "Studies on the production of insulin radio-immunoassay kit." Korea, Republic of.
@misc{etde_5269122,
title = {Studies on the production of insulin radio-immunoassay kit}
author = {Kim, J R, Kim, T H, and Kim, Y S}
abstractNote = {Insulin was labelled with Iodine-125 in about 35% yield by applying the chloramine-T method. The specific activity of the labelled product was about 100 ..mu..Ci/ug. To use the labelled product for the radioimmunoassay of insulin, the well labelled fractions were selected through a starch gel electrophoresis autoradiography, elution, and subsequent incubations with insulin antibodies. The results of the standardizations using the well labelled insulin fractions for radioimmunoassay indicated that the ratio of the antibody bound (B) to the free (F) insulin-/sup 125/I is 0.2 to 1.6 in the standard insulin dose of up to 50 ..mu..U/ml, the relatively steep dose gradient. Kits were prepared and the stabilities were also checked.}
journal = {Taehan Haekui Hakhoe Chapchi; (Korea, South)}
volume = {13:2}
journal type = {AC}
place = {Korea, Republic of}
year = {1978}
month = {Jan}
}