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Assay for plasma somatomedin: (/sup 3/H)thymidine incorporation by isolated rabbit chondrocytes

Journal Article:

Abstract

The incorporation of (/sup 3/H)thymidine by rabbit chondrocytes in vitro has been developed as a sensitive assay for plasma somatomedin. A concentration of normal plasma of 2.5% enhanced (/sup 3/H)thymidine incorporation by 5- to 20-fold compared with basal levels in the absence of plasma. The mean potency of plasma from normal adult men was 0.96 +-0.1 u./ml (mean +- S.D.) and from acromegalic patients 1.9 +- 0.4 u./ml. The apparent potency of hypopituitary plasma alone increased on heating which suggested the presence of heat-labile inhibitors of somatomedin activity. The potency of heated hypopituitary plasma (0.6 +- 0.09 u./ml) remained significantly lower (P < 0.01) than normal plasma. Human growth hormone (0.1 to 20 ..mu..u/ml), bovine growth hormone (0.5 to 20 ..mu..u/ml), insulin (0.5 to 5 ..mu..u/ml) and glucose (0.3 to 2 mmol/l) had no direct effect on the incorporation of (/sup 3/H)thymidine. Chondrocytes which had been previously stored frozen also showed a response to plasma somatomedin.
Authors:
Ashton, I K; Francis, M J.O. [1] 
  1. Nuffield Orthopaedic Centre, Oxford (UK)
Publication Date:
Jan 01, 1977
Product Type:
Journal Article
Reference Number:
AIX-09-349803; EDB-78-033999
Resource Relation:
Journal Name: J. Endocrinol.; (United Kingdom); Journal Volume: 74:2
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; PEPTIDE HORMONES; BIOASSAY; BIOCHEMICAL REACTION KINETICS; BLOOD PLASMA; CARTILAGE; LABELLING; MAN; RABBITS; STH; THYMIDINE; TRACER TECHNIQUES; TRITIUM; ANIMALS; AZINES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BODY; BODY FLUIDS; CONNECTIVE TISSUE; HETEROCYCLIC COMPOUNDS; HORMONES; HYDROGEN ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LIGHT NUCLEI; MAMMALS; NUCLEI; NUCLEOSIDES; NUCLEOTIDES; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PITUITARY HORMONES; PRIMATES; PYRIMIDINES; RADIOISOTOPES; REACTION KINETICS; RIBOSIDES; TISSUES; VERTEBRATES; YEARS LIVING RADIOISOTOPES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5264935
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JOENA
Submitting Site:
INIS
Size:
Pages: 205-212
Announcement Date:

Journal Article:

Citation Formats

Ashton, I K, and Francis, M J.O. Assay for plasma somatomedin: (/sup 3/H)thymidine incorporation by isolated rabbit chondrocytes. United Kingdom: N. p., 1977. Web.
Ashton, I K, & Francis, M J.O. Assay for plasma somatomedin: (/sup 3/H)thymidine incorporation by isolated rabbit chondrocytes. United Kingdom.
Ashton, I K, and Francis, M J.O. 1977. "Assay for plasma somatomedin: (/sup 3/H)thymidine incorporation by isolated rabbit chondrocytes." United Kingdom.
@misc{etde_5264935,
title = {Assay for plasma somatomedin: (/sup 3/H)thymidine incorporation by isolated rabbit chondrocytes}
author = {Ashton, I K, and Francis, M J.O.}
abstractNote = {The incorporation of (/sup 3/H)thymidine by rabbit chondrocytes in vitro has been developed as a sensitive assay for plasma somatomedin. A concentration of normal plasma of 2.5% enhanced (/sup 3/H)thymidine incorporation by 5- to 20-fold compared with basal levels in the absence of plasma. The mean potency of plasma from normal adult men was 0.96 +-0.1 u./ml (mean +- S.D.) and from acromegalic patients 1.9 +- 0.4 u./ml. The apparent potency of hypopituitary plasma alone increased on heating which suggested the presence of heat-labile inhibitors of somatomedin activity. The potency of heated hypopituitary plasma (0.6 +- 0.09 u./ml) remained significantly lower (P < 0.01) than normal plasma. Human growth hormone (0.1 to 20 ..mu..u/ml), bovine growth hormone (0.5 to 20 ..mu..u/ml), insulin (0.5 to 5 ..mu..u/ml) and glucose (0.3 to 2 mmol/l) had no direct effect on the incorporation of (/sup 3/H)thymidine. Chondrocytes which had been previously stored frozen also showed a response to plasma somatomedin.}
journal = {J. Endocrinol.; (United Kingdom)}
volume = {74:2}
journal type = {AC}
place = {United Kingdom}
year = {1977}
month = {Jan}
}