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Femtogram determination of ACTH by bioradioimmunoassay

Journal Article:

Abstract

A bioradioimmunoassay is described for determining adrenocorticotropic hormone (ACTH) levels in biological fluids. Slices of guinea pig's adrenal gland surviving in vitro and depleted of their cortisol content were used. When challenged with ACTH, the slices release newly synthesised cortisol into the medium which is then determined by radioimmunoasssay. A log-linear relationship was evident between added ACTH and the cortisol measured in the medium. Unlike previous methods, the sensitivity of this assay allows the determination of 0.5 femtogram (10/sup -15/g) of ACTH. A good correlation was observed when rat plasma ACTH levels were determined by the present bioassay method and by an ACTH radioimmunoassay. Mean values of plasma ACTH determined by the present method are shown for man, child, guinea pig, rabbit, rat, and hamster.
Authors:
Bruni, G; Dal Pra, P; Segre, G [1] 
  1. Siena Univ. (Italy). Inst. Pharmacology
Publication Date:
Nov 01, 1979
Product Type:
Journal Article
Reference Number:
AIX-11-525327; EDB-80-086090
Resource Relation:
Journal Name: Pharmacol. Res. Commun.; (United Kingdom); Journal Volume: 11:10
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ACTH; BIOASSAY; RADIOIMMUNOASSAY; ADRENAL HORMONES; ANIMALS; BIOLOGICAL MATERIALS; BLOOD; BODY FLUIDS; CORTICOSTEROIDS; GLUCOCORTICOIDS; HORMONES; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; KETONES; MAMMALS; MATERIALS; ORGANIC COMPOUNDS; PEPTIDE HORMONES; PITUITARY HORMONES; PREGNANES; PRIMATES; RADIOASSAY; RODENTS; STEROID HORMONES; STEROIDS; TRACER TECHNIQUES; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5264642
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: PLRCA
Submitting Site:
INIS
Size:
Pages: 853-859
Announcement Date:

Journal Article:

Citation Formats

Bruni, G, Dal Pra, P, and Segre, G. Femtogram determination of ACTH by bioradioimmunoassay. United Kingdom: N. p., 1979. Web.
Bruni, G, Dal Pra, P, & Segre, G. Femtogram determination of ACTH by bioradioimmunoassay. United Kingdom.
Bruni, G, Dal Pra, P, and Segre, G. 1979. "Femtogram determination of ACTH by bioradioimmunoassay." United Kingdom.
@misc{etde_5264642,
title = {Femtogram determination of ACTH by bioradioimmunoassay}
author = {Bruni, G, Dal Pra, P, and Segre, G}
abstractNote = {A bioradioimmunoassay is described for determining adrenocorticotropic hormone (ACTH) levels in biological fluids. Slices of guinea pig's adrenal gland surviving in vitro and depleted of their cortisol content were used. When challenged with ACTH, the slices release newly synthesised cortisol into the medium which is then determined by radioimmunoasssay. A log-linear relationship was evident between added ACTH and the cortisol measured in the medium. Unlike previous methods, the sensitivity of this assay allows the determination of 0.5 femtogram (10/sup -15/g) of ACTH. A good correlation was observed when rat plasma ACTH levels were determined by the present bioassay method and by an ACTH radioimmunoassay. Mean values of plasma ACTH determined by the present method are shown for man, child, guinea pig, rabbit, rat, and hamster.}
journal = {Pharmacol. Res. Commun.; (United Kingdom)}
volume = {11:10}
journal type = {AC}
place = {United Kingdom}
year = {1979}
month = {Nov}
}