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Mutagenesis of metal compounds in bacteria

Journal Article:

Abstract

The mutagenic activity of 41 metal compounds was examined by applying the Rec-assay method with Bacillus subtilis H17 (rec/sup +/) and M45 (rec/sup -/) strains. Among these compounds, Na/sub 2/HAsO/sub 4/, CdCl/sub 2/, K/sub 2/CrO/sub 4/, K/sub 2/Cr/sub 2/O/sub 7/, CH/sub 3/HgCl, C/sub 2/H/sub 5/HgCl, CH/sub 3/COOHgC/sub 6/H/sub 5/, MnCl/sub 2/, MnNO/sub 3/, MnSO/sub 4/, Mn(CH/sub 3/COO)/sub 2/, (NH/sub 4/)/sub 2/MoO/sub 4/ and KMoO/sub 4/ showed positive results. The reactions of K/sub 2/Cr/sub 2/O/sub 7/ and (NH/sub 4/)/sub 2/MoO/sub 4/ were especially strong in the assay. Therefore, mutation induction to reversion (try/sup +/) and streptomycin resistance (SM/sup r/) of E. coli B/r WP2 try/sup -/ (hcl/sup +/ and hcr/sup -/) by the two compounds were examined by the following two experimental procedures. Stationary phase bacteria were exposed to the compounds at high concentrations (6.9 x 10/sup -3/ approx. 3.44 x 10/sup -2/M) in M9 buffer for 15 min at 37/sup -/ with shaking. After incubation at 37/sup 0/ for 48 h visible colonies on the plates were scored. Bacteria in M9 buffer were plated in media supplemented with low concentrations (1.7 x 10/sup -5/ approx. 3.4 x 10/sup -5/M) of the compounds. K/sub 2/Cr/sub 2/O/sub 7/ and (NH/sub 4/)/sub 2/MoO/sub  More>>
Authors:
Publication Date:
Jan 01, 1974
Product Type:
Journal Article
Reference Number:
EDB-85-159160
Resource Relation:
Journal Name: Mutat. Res.; (Netherlands); Journal Volume: 26
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; AMMONIUM COMPOUNDS; MUTAGEN SCREENING; ARSENATES; CADMIUM CHLORIDES; CHROMATES; MANGANESE CHLORIDES; MANGANESE NITRATES; MANGANESE SULFATES; METHYLMERCURY; MOLYBDATES; ORGANIC MERCURY COMPOUNDS; POTASSIUM PERMANGANATES; BACILLUS SUBTILIS; ESCHERICHIA COLI; ALKALI METAL COMPOUNDS; ARSENIC COMPOUNDS; BACILLUS; BACTERIA; CADMIUM COMPOUNDS; CADMIUM HALIDES; CHLORIDES; CHLORINE COMPOUNDS; CHROMIUM COMPOUNDS; HALIDES; HALOGEN COMPOUNDS; MANGANESE COMPOUNDS; MANGANESE HALIDES; MERCURY COMPOUNDS; MICROORGANISMS; MOLYBDENUM COMPOUNDS; NITRATES; NITROGEN COMPOUNDS; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; PERMANGANATES; POTASSIUM COMPOUNDS; REFRACTORY METAL COMPOUNDS; SCREENING; SULFATES; SULFUR COMPOUNDS; TRANSITION ELEMENT COMPOUNDS; 560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)
OSTI ID:
5264257
Research Organizations:
Doshisha Univ., Kyoto, Japan
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: MUREA
Submitting Site:
HEDB
Size:
Pages: 437-438
Announcement Date:

Journal Article:

Citation Formats

Nishioka, H. Mutagenesis of metal compounds in bacteria. Netherlands: N. p., 1974. Web.
Nishioka, H. Mutagenesis of metal compounds in bacteria. Netherlands.
Nishioka, H. 1974. "Mutagenesis of metal compounds in bacteria." Netherlands.
@misc{etde_5264257,
title = {Mutagenesis of metal compounds in bacteria}
author = {Nishioka, H}
abstractNote = {The mutagenic activity of 41 metal compounds was examined by applying the Rec-assay method with Bacillus subtilis H17 (rec/sup +/) and M45 (rec/sup -/) strains. Among these compounds, Na/sub 2/HAsO/sub 4/, CdCl/sub 2/, K/sub 2/CrO/sub 4/, K/sub 2/Cr/sub 2/O/sub 7/, CH/sub 3/HgCl, C/sub 2/H/sub 5/HgCl, CH/sub 3/COOHgC/sub 6/H/sub 5/, MnCl/sub 2/, MnNO/sub 3/, MnSO/sub 4/, Mn(CH/sub 3/COO)/sub 2/, (NH/sub 4/)/sub 2/MoO/sub 4/ and KMoO/sub 4/ showed positive results. The reactions of K/sub 2/Cr/sub 2/O/sub 7/ and (NH/sub 4/)/sub 2/MoO/sub 4/ were especially strong in the assay. Therefore, mutation induction to reversion (try/sup +/) and streptomycin resistance (SM/sup r/) of E. coli B/r WP2 try/sup -/ (hcl/sup +/ and hcr/sup -/) by the two compounds were examined by the following two experimental procedures. Stationary phase bacteria were exposed to the compounds at high concentrations (6.9 x 10/sup -3/ approx. 3.44 x 10/sup -2/M) in M9 buffer for 15 min at 37/sup -/ with shaking. After incubation at 37/sup 0/ for 48 h visible colonies on the plates were scored. Bacteria in M9 buffer were plated in media supplemented with low concentrations (1.7 x 10/sup -5/ approx. 3.4 x 10/sup -5/M) of the compounds. K/sub 2/Cr/sub 2/O/sub 7/ and (NH/sub 4/)/sub 2/MoO/sub 4/ increased the mutation rate of SM/sup r/ and try/sup +/ in both strains treated with either procedure. No marked differences in mutation rate were found between hcr/sup +/ and hcr/sup -/. After treatment with high concentrations of compounds one can imagine that a peroxidation state produced by these peroxides in the media might affect the killing and mutation induction. These results suggest the possibility that the mutagenesis of the metals relate to their atomic values, rather than the peroxidation state as far as these two compounds are concerned.}
journal = {Mutat. Res.; (Netherlands)}
volume = {26}
journal type = {AC}
place = {Netherlands}
year = {1974}
month = {Jan}
}