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Determination of serum digosin. A comparison between RIA and EIA

Abstract

The results of two radioimmunoassays (RIA, precipitating technique), of a homogenous (EMIT) and a heterogenous (ELISA) enzyme immunoassay (EIA) for ascertaining the amounts of digoxin showed a good correlation in precision and a reasonably AK satisfying correlation in the recovery. However, there was a clear discrepancy in the amounts of digoxin concentrate in the serum of patients. Only the RIA of Abbott and the EIA of Boehringer showed no significant differences. Particularly noticeable was the tendency towards lower values in the EMIT-technique as well as its liability to unspecific serum changes (lipaemia etc.), which often made the detection of digoxin impossible. The routine use of this technique appears problematic. The need for establishing one's own laboratory and test-specific therapeutical range is pointed out.
Authors:
Spitz, J; Braun, J S; Schmidt, M; [1]  Abt fuer Labormedizin, Krankenhausstiftung Bamberg [2] 
  1. Krankenhausstiftung Bamberg (Germany, F.R.). Nuklearmedizinische Abt.
  2. Germany, F.R.
Publication Date:
Dec 01, 1979
Product Type:
Journal Article
Reference Number:
AIX-11-544351; EDB-80-103260
Resource Relation:
Journal Name: Nucl.-Med. (Stuttgart); (Germany, Federal Republic of); Journal Volume: 18:5
Subject:
59 BASIC BIOLOGICAL SCIENCES; DIGOXIN; RADIOIMMUNOASSAY; ACCURACY; COMPARATIVE EVALUATIONS; ENZYMES; PATIENTS; CARBOHYDRATES; CARDIOTONICS; DIGITALIS GLYCOSIDES; DRUGS; GLYCOSIDES; ISOTOPE APPLICATIONS; ORGANIC COMPOUNDS; RADIOASSAY; STEROIDS; TRACER TECHNIQUES; 550201* - Biochemistry- Tracer Techniques
OSTI ID:
5253948
Country of Origin:
Germany
Language:
German
Other Identifying Numbers:
Journal ID: CODEN: NMIMA
Submitting Site:
INIS
Size:
Pages: 237-240
Announcement Date:
Aug 01, 1980

Citation Formats

Spitz, J, Braun, J S, Schmidt, M, and Abt fuer Labormedizin, Krankenhausstiftung Bamberg. Determination of serum digosin. A comparison between RIA and EIA. Germany: N. p., 1979. Web.
Spitz, J, Braun, J S, Schmidt, M, & Abt fuer Labormedizin, Krankenhausstiftung Bamberg. Determination of serum digosin. A comparison between RIA and EIA. Germany.
Spitz, J, Braun, J S, Schmidt, M, and Abt fuer Labormedizin, Krankenhausstiftung Bamberg. 1979. "Determination of serum digosin. A comparison between RIA and EIA." Germany.
@misc{etde_5253948,
title = {Determination of serum digosin. A comparison between RIA and EIA}
author = {Spitz, J, Braun, J S, Schmidt, M, and Abt fuer Labormedizin, Krankenhausstiftung Bamberg}
abstractNote = {The results of two radioimmunoassays (RIA, precipitating technique), of a homogenous (EMIT) and a heterogenous (ELISA) enzyme immunoassay (EIA) for ascertaining the amounts of digoxin showed a good correlation in precision and a reasonably AK satisfying correlation in the recovery. However, there was a clear discrepancy in the amounts of digoxin concentrate in the serum of patients. Only the RIA of Abbott and the EIA of Boehringer showed no significant differences. Particularly noticeable was the tendency towards lower values in the EMIT-technique as well as its liability to unspecific serum changes (lipaemia etc.), which often made the detection of digoxin impossible. The routine use of this technique appears problematic. The need for establishing one's own laboratory and test-specific therapeutical range is pointed out.}
journal = []
volume = {18:5}
journal type = {AC}
place = {Germany}
year = {1979}
month = {Dec}
}