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Studies on the irradiation of toxins of Clostridium botulinum and Staphylococcus aureus

Journal Article:

Abstract

The effects of irradiation of Clostridium botulinum neurotoxin type A (BNTA) and staphylococcal enterotoxin A (SEA) in gelatin phosphate buffer and cooked mince beef slurries were investigated. Estimation of toxins by immunoassays showed that in buffer, toxins were destroyed by irradiation at 8.0 kGy; in mince slurries however, 45% of BTNA and 27-34% of SEA remained after this level of irradiation. At 23.7 kGy, over twice the dose of irradiation proposed for legal acceptance in the UK, 15% of BNTA and 16-26% of SEA still remained. Increasing concentrations of mince conferred increased protection against the effect of irradiation on both toxins. The biological activity of BNTA was more sensitive to irradiation than the immunological activity. Staphylococcal enterotoxin was more resistant to irradiation than BNTA. Irradiation should therefore only be used in conjunction with good manufacturing practices to prevent microbial proliferation and toxin production prior to irradiation. (author).
Authors:
Rose, S A; Bailey, N E; Stringer, M F; [1]  Modi, N K; Tranter, H S; [2]  Hambleton, P [3] 
  1. Campden Food and Drink Research Association, Chipping Campden (UK)
  2. Porton International plc., London (UK)
  3. Centre for Applied Microbiological Research, Porton Down (UK)
Publication Date:
Oct 01, 1988
Product Type:
Journal Article
Reference Number:
AIX-21-010262; EDB-90-011427
Resource Relation:
Journal Name: Journal of Applied Bacteriology; (UK); Journal Volume: 65:4
Subject:
60 APPLIED LIFE SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; RADIOSTERILIZATION; ENDOTOXINS; CLOSTRIDIUM; RADIATION DOSES; STAPHYLOCOCCUS; ANTIGENS; BACTERIA; DOSES; MATERIALS; MICROORGANISMS; STERILIZATION; TOXIC MATERIALS; TOXINS; 553004* - Agriculture & Food Technology- Food Protection & Preservation- (1987-); 560130 - Radiation Effects on Microorganisms
OSTI ID:
5252451
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0021-8847; CODEN: JABAA
Submitting Site:
GBN
Size:
Pages: 223-229
Announcement Date:

Journal Article:

Citation Formats

Rose, S A, Bailey, N E, Stringer, M F, Modi, N K, Tranter, H S, and Hambleton, P. Studies on the irradiation of toxins of Clostridium botulinum and Staphylococcus aureus. United Kingdom: N. p., 1988. Web.
Rose, S A, Bailey, N E, Stringer, M F, Modi, N K, Tranter, H S, & Hambleton, P. Studies on the irradiation of toxins of Clostridium botulinum and Staphylococcus aureus. United Kingdom.
Rose, S A, Bailey, N E, Stringer, M F, Modi, N K, Tranter, H S, and Hambleton, P. 1988. "Studies on the irradiation of toxins of Clostridium botulinum and Staphylococcus aureus." United Kingdom.
@misc{etde_5252451,
title = {Studies on the irradiation of toxins of Clostridium botulinum and Staphylococcus aureus}
author = {Rose, S A, Bailey, N E, Stringer, M F, Modi, N K, Tranter, H S, and Hambleton, P}
abstractNote = {The effects of irradiation of Clostridium botulinum neurotoxin type A (BNTA) and staphylococcal enterotoxin A (SEA) in gelatin phosphate buffer and cooked mince beef slurries were investigated. Estimation of toxins by immunoassays showed that in buffer, toxins were destroyed by irradiation at 8.0 kGy; in mince slurries however, 45% of BTNA and 27-34% of SEA remained after this level of irradiation. At 23.7 kGy, over twice the dose of irradiation proposed for legal acceptance in the UK, 15% of BNTA and 16-26% of SEA still remained. Increasing concentrations of mince conferred increased protection against the effect of irradiation on both toxins. The biological activity of BNTA was more sensitive to irradiation than the immunological activity. Staphylococcal enterotoxin was more resistant to irradiation than BNTA. Irradiation should therefore only be used in conjunction with good manufacturing practices to prevent microbial proliferation and toxin production prior to irradiation. (author).}
journal = {Journal of Applied Bacteriology; (UK)}
volume = {65:4}
journal type = {AC}
place = {United Kingdom}
year = {1988}
month = {Oct}
}