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Timing of RNA synthesis for sperimiogenesis in organ cultures of Drosophila melanogaster teste

Abstract

A method for the organ culture of Drosophila testes is described which supports the differentiation of primary spermatocytes through the meiotic divisions to elongating spermatids. Autoradiographic and inhibitor studies reveal no evidence for RNA synthesis by developing spermatids of Drosophila melanogaster; most, if not all, of the RNA required for the differentiation and elongation of sperm is synthesized earlier in the primary spermatocytes. Primary spermatocytes will differentiate into elongating spermatids in organ culture, despite severe (96 to 98%) inhibition of /sup 3/H-uridine incorporation into RNA effected by 50 ..mu..g/ml 3'-deoxyadenosine. Protein synthesis in spermatids continues to be active in the presence of 3'-deoxyadenosine, but that in growing spermatocytes is severely inhibited.
Publication Date:
Jan 01, 1974
Product Type:
Journal Article
Reference Number:
EDB-82-131598
Resource Relation:
Journal Name: Wilhelm Roux' Arch. Entwicklungsmech. Org.; (Germany, Federal Republic of); Journal Volume: 174
Subject:
59 BASIC BIOLOGICAL SCIENCES; DROSOPHILA; SPERMATOGENESIS; PROTEINS; BIOSYNTHESIS; RNA; SPERMATOCYTES; CELL DIFFERENTIATION; AUTORADIOGRAPHY; INHIBITION; NUCLEOSIDES; SPERMATOZOA; TESTES; TIME DEPENDENCE; TISSUE CULTURES; TRITIUM COMPOUNDS; ANIMALS; ARTHROPODS; BODY; DIPTERA; FLIES; FRUIT FLIES; GAMETES; GAMETOGENESIS; GERM CELLS; GONADS; INSECTS; INVERTEBRATES; LABELLED COMPOUNDS; MALE GENITALS; NUCLEIC ACIDS; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANS; RIBOSIDES; SYNTHESIS; 551001* - Physiological Systems- Tracer Techniques; 550301 - Cytology- Tracer Techniques
OSTI ID:
5242314
Research Organizations:
Univ. of California, San Diego
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: WREOA
Submitting Site:
HEDB
Size:
Pages: 133-148
Announcement Date:

Citation Formats

Gould-Somero, M, and Holland, L. Timing of RNA synthesis for sperimiogenesis in organ cultures of Drosophila melanogaster teste. Germany: N. p., 1974. Web. doi:10.1007/BF00573626.
Gould-Somero, M, & Holland, L. Timing of RNA synthesis for sperimiogenesis in organ cultures of Drosophila melanogaster teste. Germany. doi:10.1007/BF00573626.
Gould-Somero, M, and Holland, L. 1974. "Timing of RNA synthesis for sperimiogenesis in organ cultures of Drosophila melanogaster teste." Germany. doi:10.1007/BF00573626. https://www.osti.gov/servlets/purl/10.1007/BF00573626.
@misc{etde_5242314,
title = {Timing of RNA synthesis for sperimiogenesis in organ cultures of Drosophila melanogaster teste}
author = {Gould-Somero, M, and Holland, L}
abstractNote = {A method for the organ culture of Drosophila testes is described which supports the differentiation of primary spermatocytes through the meiotic divisions to elongating spermatids. Autoradiographic and inhibitor studies reveal no evidence for RNA synthesis by developing spermatids of Drosophila melanogaster; most, if not all, of the RNA required for the differentiation and elongation of sperm is synthesized earlier in the primary spermatocytes. Primary spermatocytes will differentiate into elongating spermatids in organ culture, despite severe (96 to 98%) inhibition of /sup 3/H-uridine incorporation into RNA effected by 50 ..mu..g/ml 3'-deoxyadenosine. Protein synthesis in spermatids continues to be active in the presence of 3'-deoxyadenosine, but that in growing spermatocytes is severely inhibited.}
doi = {10.1007/BF00573626}
journal = {Wilhelm Roux' Arch. Entwicklungsmech. Org.; (Germany, Federal Republic of)}
volume = {174}
journal type = {AC}
place = {Germany}
year = {1974}
month = {Jan}
}