Abstract
Polycyclic aromatic hydrocarbons (PAH) have been identified as genotoxic pollutants in sediment from the Black River, Ohio, where a high incidence of hepatoma was observed in brown bullhead catfish (Ictalurus nebulosus). Subfractions of PAH based on the number of aromatic carbons were isolated from the PAH fraction of a Black River sediment extract. Ten subfractions were analyzed by capillary column gas chromatography-mass spectrometry, tested for mutagenicity using the Ames assay, and tested for induction of unscheduled DNA synthesis (UDS) in primary rat hepatocytes. The Ames assay indicated significant mutagenic activity in fractions which contained PAH with 4-6 aromatic rings; the majority of the activity was found in the fraction composed of 5-ring compounds. UDS was also significant in the same fractions, although the greatest genotoxicity was observed in the cata-condensed and peri-condensed 4-ring fractions, which contained a large amount of alkylated-PAH.
Citation Formats
West, W R, Smith, P A, Booth, G M, Wise, S A, and Lee, M L.
Determination of genotoxic polycyclic aromatic hydrocarbons in a sediment from the Black River (Ohio). [Ictalurus nebulosus].
Germany: N. p.,
1986.
Web.
doi:10.1007/BF01061100.
West, W R, Smith, P A, Booth, G M, Wise, S A, & Lee, M L.
Determination of genotoxic polycyclic aromatic hydrocarbons in a sediment from the Black River (Ohio). [Ictalurus nebulosus].
Germany.
https://doi.org/10.1007/BF01061100
West, W R, Smith, P A, Booth, G M, Wise, S A, and Lee, M L.
1986.
"Determination of genotoxic polycyclic aromatic hydrocarbons in a sediment from the Black River (Ohio). [Ictalurus nebulosus]."
Germany.
https://doi.org/10.1007/BF01061100.
@misc{etde_5234937,
title = {Determination of genotoxic polycyclic aromatic hydrocarbons in a sediment from the Black River (Ohio). [Ictalurus nebulosus]}
author = {West, W R, Smith, P A, Booth, G M, Wise, S A, and Lee, M L}
abstractNote = {Polycyclic aromatic hydrocarbons (PAH) have been identified as genotoxic pollutants in sediment from the Black River, Ohio, where a high incidence of hepatoma was observed in brown bullhead catfish (Ictalurus nebulosus). Subfractions of PAH based on the number of aromatic carbons were isolated from the PAH fraction of a Black River sediment extract. Ten subfractions were analyzed by capillary column gas chromatography-mass spectrometry, tested for mutagenicity using the Ames assay, and tested for induction of unscheduled DNA synthesis (UDS) in primary rat hepatocytes. The Ames assay indicated significant mutagenic activity in fractions which contained PAH with 4-6 aromatic rings; the majority of the activity was found in the fraction composed of 5-ring compounds. UDS was also significant in the same fractions, although the greatest genotoxicity was observed in the cata-condensed and peri-condensed 4-ring fractions, which contained a large amount of alkylated-PAH.}
doi = {10.1007/BF01061100}
journal = []
volume = {15:3}
journal type = {AC}
place = {Germany}
year = {1986}
month = {May}
}
title = {Determination of genotoxic polycyclic aromatic hydrocarbons in a sediment from the Black River (Ohio). [Ictalurus nebulosus]}
author = {West, W R, Smith, P A, Booth, G M, Wise, S A, and Lee, M L}
abstractNote = {Polycyclic aromatic hydrocarbons (PAH) have been identified as genotoxic pollutants in sediment from the Black River, Ohio, where a high incidence of hepatoma was observed in brown bullhead catfish (Ictalurus nebulosus). Subfractions of PAH based on the number of aromatic carbons were isolated from the PAH fraction of a Black River sediment extract. Ten subfractions were analyzed by capillary column gas chromatography-mass spectrometry, tested for mutagenicity using the Ames assay, and tested for induction of unscheduled DNA synthesis (UDS) in primary rat hepatocytes. The Ames assay indicated significant mutagenic activity in fractions which contained PAH with 4-6 aromatic rings; the majority of the activity was found in the fraction composed of 5-ring compounds. UDS was also significant in the same fractions, although the greatest genotoxicity was observed in the cata-condensed and peri-condensed 4-ring fractions, which contained a large amount of alkylated-PAH.}
doi = {10.1007/BF01061100}
journal = []
volume = {15:3}
journal type = {AC}
place = {Germany}
year = {1986}
month = {May}
}