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Affinity chromatography of tetanus toxin, tetanus toxoid, and botulinum A toxin on synaptosomes, and differentiation of their acceptors

Journal Article:

Abstract

/sup 125/I-labelled tetanus toxin and /sup 125/I-labelled botulinum A neurotoxin are known to be specifically bound to brain synaptosomes. In order to discriminate between active toxin and inactive admixtures present in the starting material or arising during iodination, synaptosome columns were prepared using bromacetylcellulose and/or kieselgur (Celite) as carriers. Both types of columns adsorb the toxins from low ionic strength medium and release them if the pH and ionic strength are raised. Botulinum toxin was eluted with lower ionic strength than tetanus toxin, and could be freed from nontoxic admixtures. Analysis by affinity chromatography disclosed partially toxoided tetanus toxin in both labelled and unlabelled toxin samples. High concentrations of formaldehyde (0.5%) destroyed both toxicity and affinity to the synaptosomes of tetanus toxin. Low concentrations of formaldehyde (0.05%) yielded a derivative of low toxicity which was still, however less firmly, bound to synaptosomes. Tetanus and botulinum toxin differ by their acceptors. Whereas unlabelled botulinum toxin is unable to compete with labelled tetanus toxin, unlabelled tetanus toxin slightly competes with botulinum toxin. Both labelled toxins display anomalous binding behaviour in that they cannot be displaced completely even with a large excess of unlabelled toxin.
Authors:
Habermann, E [1] 
  1. Giessen Univ. (Germany, F.R.). Pharmakologisches Inst.
Publication Date:
Jan 01, 1976
Product Type:
Journal Article
Reference Number:
AIX-09-352102; EDB-78-051633
Resource Relation:
Journal Name: Naunyn-Schmiedebergs Arch. Pharmakol.; (Germany, Federal Republic of); Journal Volume: 293:1
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; BRAIN; BIOCHEMICAL REACTION KINETICS; CLOSTRIDIUM BOTULINUM; RADIOCHROMATOGRAPHY; NEUROLOGY; TRACER TECHNIQUES; TETANUS; TOXINS; IN VITRO; IODINE 125; LABELLING; MICE; NERVE CELLS; UPTAKE; ANIMAL CELLS; ANIMALS; ANTIGENS; BACTERIA; BETA DECAY RADIOISOTOPES; BODY; CENTRAL NERVOUS SYSTEM; CHROMATOGRAPHY; CLOSTRIDIUM; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; INFECTIOUS DISEASES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; MAMMALS; MEDICINE; MICROORGANISMS; NERVOUS SYSTEM; NUCLEI; ODD-EVEN NUCLEI; ORGANS; RADIOISOTOPES; REACTION KINETICS; RODENTS; SEPARATION PROCESSES; SOMATIC CELLS; TOXIC MATERIALS; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5227489
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: NNAPB
Submitting Site:
INIS
Size:
Pages: 1-9
Announcement Date:

Journal Article:

Citation Formats

Habermann, E. Affinity chromatography of tetanus toxin, tetanus toxoid, and botulinum A toxin on synaptosomes, and differentiation of their acceptors. Germany: N. p., 1976. Web.
Habermann, E. Affinity chromatography of tetanus toxin, tetanus toxoid, and botulinum A toxin on synaptosomes, and differentiation of their acceptors. Germany.
Habermann, E. 1976. "Affinity chromatography of tetanus toxin, tetanus toxoid, and botulinum A toxin on synaptosomes, and differentiation of their acceptors." Germany.
@misc{etde_5227489,
title = {Affinity chromatography of tetanus toxin, tetanus toxoid, and botulinum A toxin on synaptosomes, and differentiation of their acceptors}
author = {Habermann, E}
abstractNote = {/sup 125/I-labelled tetanus toxin and /sup 125/I-labelled botulinum A neurotoxin are known to be specifically bound to brain synaptosomes. In order to discriminate between active toxin and inactive admixtures present in the starting material or arising during iodination, synaptosome columns were prepared using bromacetylcellulose and/or kieselgur (Celite) as carriers. Both types of columns adsorb the toxins from low ionic strength medium and release them if the pH and ionic strength are raised. Botulinum toxin was eluted with lower ionic strength than tetanus toxin, and could be freed from nontoxic admixtures. Analysis by affinity chromatography disclosed partially toxoided tetanus toxin in both labelled and unlabelled toxin samples. High concentrations of formaldehyde (0.5%) destroyed both toxicity and affinity to the synaptosomes of tetanus toxin. Low concentrations of formaldehyde (0.05%) yielded a derivative of low toxicity which was still, however less firmly, bound to synaptosomes. Tetanus and botulinum toxin differ by their acceptors. Whereas unlabelled botulinum toxin is unable to compete with labelled tetanus toxin, unlabelled tetanus toxin slightly competes with botulinum toxin. Both labelled toxins display anomalous binding behaviour in that they cannot be displaced completely even with a large excess of unlabelled toxin.}
journal = {Naunyn-Schmiedebergs Arch. Pharmakol.; (Germany, Federal Republic of)}
volume = {293:1}
journal type = {AC}
place = {Germany}
year = {1976}
month = {Jan}
}