You need JavaScript to view this

Role of DNA polymerase I in liquid holding recovery of uv-irradiated Escherichia coli

Journal Article:

Abstract

Excision of cyclobutyl dipyrimidines from, and accumulation of strand interruptions in DNA of different strains of E.coli K12 were determined during liquid holding recovery after uv irradiation. The extent of Pyr <> Pyr excision was the same (20 to 25%) for both a polA mutant (E.coli P3478) and its parental wild type strain (E.coli W3110); however, single strand interruptions accumulated during liquid holding of polA cells, but not in the parental strain. In contrast, excision was greatly reduced in a mutant (KMBL 1789) which is defective in the 5' ..-->.. 3' exonucleolytic function of DNA polymerase I. These data suggest that excision and resynthesis during liquid holding are carried out primarily, if not entirely, by DNA polymerase I. It is further concluded that excision alone is both a necessary and sufficient condition to elicit liquid holding recovery, and that this excision requires a functional polymerase I 5' ..-->.. 3' exonuclease.
Authors:
Tang, M S; Patrick, M H [1] 
  1. Texas Univ., Dallas (USA)
Publication Date:
Sep 01, 1977
Product Type:
Journal Article
Reference Number:
AIX-09-352007; EDB-78-051666
Resource Relation:
Journal Name: Photochem. Photobiol.; (United Kingdom); Journal Volume: 26:3
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA; STRAND BREAKS; POLYMERASES; REACTION KINETICS; LIQUID HOLDING RECOVERY; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; CHEMICAL RADIATION EFFECTS; DIMERS; ESCHERICHIA COLI; PHOTOSENSITIVITY; PYRIMIDINES; ULTRAVIOLET RADIATION; AZINES; BACTERIA; CHEMISTRY; ELECTROMAGNETIC RADIATION; ENZYMES; HETEROCYCLIC COMPOUNDS; KINETICS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; RADIATION CHEMISTRY; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; SENSITIVITY; 560112* - Radiation Effects on Biochemicals- In Microorganisms- (-1987); 560131 - Radiation Effects on Microorganisms- Basic Studies- (-1987)
OSTI ID:
5227339
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: PHCBA
Submitting Site:
INIS
Size:
Pages: 257-262
Announcement Date:

Journal Article:

Citation Formats

Tang, M S, and Patrick, M H. Role of DNA polymerase I in liquid holding recovery of uv-irradiated Escherichia coli. United Kingdom: N. p., 1977. Web.
Tang, M S, & Patrick, M H. Role of DNA polymerase I in liquid holding recovery of uv-irradiated Escherichia coli. United Kingdom.
Tang, M S, and Patrick, M H. 1977. "Role of DNA polymerase I in liquid holding recovery of uv-irradiated Escherichia coli." United Kingdom.
@misc{etde_5227339,
title = {Role of DNA polymerase I in liquid holding recovery of uv-irradiated Escherichia coli}
author = {Tang, M S, and Patrick, M H}
abstractNote = {Excision of cyclobutyl dipyrimidines from, and accumulation of strand interruptions in DNA of different strains of E.coli K12 were determined during liquid holding recovery after uv irradiation. The extent of Pyr <> Pyr excision was the same (20 to 25%) for both a polA mutant (E.coli P3478) and its parental wild type strain (E.coli W3110); however, single strand interruptions accumulated during liquid holding of polA cells, but not in the parental strain. In contrast, excision was greatly reduced in a mutant (KMBL 1789) which is defective in the 5' ..-->.. 3' exonucleolytic function of DNA polymerase I. These data suggest that excision and resynthesis during liquid holding are carried out primarily, if not entirely, by DNA polymerase I. It is further concluded that excision alone is both a necessary and sufficient condition to elicit liquid holding recovery, and that this excision requires a functional polymerase I 5' ..-->.. 3' exonuclease.}
journal = {Photochem. Photobiol.; (United Kingdom)}
volume = {26:3}
journal type = {AC}
place = {United Kingdom}
year = {1977}
month = {Sep}
}