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Use of sodium salicylate as a blocking agent for cortisol-binding-globulin in a radioimmunoassay for cortisol on unextracted plasma

Journal Article:

Abstract

This report describes investigations into the use of sodium salicylate as a cortisol-binding-globulin blocking agent and the subsequent development of a radioimmunoassay for cortisol on unextracted plasma. Cortisol antiserum was raised against a cortisol 3-0-(carboxy-methyl) oxime-bovine serum albumin conjugate. A /sup 125/I-labelled cortisol-tyrosine methyl ester conjugate was also prepared for use in the assay. The radioimmunoassay developed involved no pre-treatment or extraction of the samples before analysis and was extremely simple to perform. Comparison with another radioimmunoassay for cortisol and with the Mattingly fluorimetric assay gave good correlation.
Authors:
Kane, J W [1] 
  1. Withington Hospital, Manchester (UK)
Publication Date:
Jul 01, 1979
Product Type:
Journal Article
Reference Number:
AIX-11-502517; EDB-80-098203
Resource Relation:
Journal Name: Ann. Clin. Biochem.; (United Kingdom); Journal Volume: 16
Subject:
59 BASIC BIOLOGICAL SCIENCES; HYDROCORTISONE; RADIOIMMUNOASSAY; BLOOD PLASMA; CHEMICAL BONDS; GLOBULINS; IMMUNE SERUMS; INHIBITION; IODINE 125; LABELLED COMPOUNDS; SALICYLIC ACID; SODIUM COMPOUNDS; TYROSINE; ADRENAL HORMONES; ALKALI METAL COMPOUNDS; AMINO ACIDS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BODY FLUIDS; CARBOXYLIC ACIDS; CORTICOSTEROIDS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; GLUCOCORTICOIDS; HORMONES; HYDROXY ACIDS; HYDROXY COMPOUNDS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KETONES; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; PREGNANES; PROTEINS; RADIOASSAY; RADIOISOTOPES; STEROID HORMONES; STEROIDS; TRACER TECHNIQUES; 550201* - Biochemistry- Tracer Techniques
OSTI ID:
5212870
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: ACBOB
Submitting Site:
INIS
Size:
Pages: 209-212
Announcement Date:

Journal Article:

Citation Formats

Kane, J W. Use of sodium salicylate as a blocking agent for cortisol-binding-globulin in a radioimmunoassay for cortisol on unextracted plasma. United Kingdom: N. p., 1979. Web.
Kane, J W. Use of sodium salicylate as a blocking agent for cortisol-binding-globulin in a radioimmunoassay for cortisol on unextracted plasma. United Kingdom.
Kane, J W. 1979. "Use of sodium salicylate as a blocking agent for cortisol-binding-globulin in a radioimmunoassay for cortisol on unextracted plasma." United Kingdom.
@misc{etde_5212870,
title = {Use of sodium salicylate as a blocking agent for cortisol-binding-globulin in a radioimmunoassay for cortisol on unextracted plasma}
author = {Kane, J W}
abstractNote = {This report describes investigations into the use of sodium salicylate as a cortisol-binding-globulin blocking agent and the subsequent development of a radioimmunoassay for cortisol on unextracted plasma. Cortisol antiserum was raised against a cortisol 3-0-(carboxy-methyl) oxime-bovine serum albumin conjugate. A /sup 125/I-labelled cortisol-tyrosine methyl ester conjugate was also prepared for use in the assay. The radioimmunoassay developed involved no pre-treatment or extraction of the samples before analysis and was extremely simple to perform. Comparison with another radioimmunoassay for cortisol and with the Mattingly fluorimetric assay gave good correlation.}
journal = {Ann. Clin. Biochem.; (United Kingdom)}
volume = {16}
journal type = {AC}
place = {United Kingdom}
year = {1979}
month = {Jul}
}