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Comparison of radioimmunoassay and ELISA methods for detection of antibodies to chromatin components

Journal Article:

Abstract

A solid phase radioimmunoassay has been compared with an enzyme-linked immunosorbent assay (ELISA) for efficacy in measuring anti-chromatin antibodies. The low backgrounds achieved with the radioimmunoassay method produced a high signal-to-noise ratio and enabled detection of the human test antiserum at a dilution of 1:102,400. By contrast, the ELISA could detect the same antiserum only at a dilution of 1;3200 and above. The radioimmunoassay was consistently more sensitive than the ELSIA for detection of anti-chromatin antibodies in a number of human and mouse sera and ascites fluid containing a monoclonal antibody. Factors affecting sensitivity in both assays are discussed.
Authors:
Fowler, E; Cheng, N [1] 
  1. North Carolina Univ., Chapel Hill (USA). Dept. of Bacteriology and Immunology
Publication Date:
Sep 16, 1983
Product Type:
Journal Article
Reference Number:
AIX-15-001885; EDB-84-051670
Resource Relation:
Journal Name: J. Immunol. Methods; (Netherlands); Journal Volume: 62:3
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; RADIOIMMUNOASSAY; COMPARATIVE EVALUATIONS; BACKGROUND NOISE; CHEMICAL BONDS; CHROMATIN; IODINE 125; RADIOENZYMATIC ASSAY; SENSITIVITY; BETA DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NOISE; NUCLEI; ODD-EVEN NUCLEI; RADIOASSAY; RADIOISOTOPES; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5212687
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JIMMB
Submitting Site:
HEDB
Size:
Pages: 297-303
Announcement Date:
Feb 01, 1984

Journal Article:

Citation Formats

Fowler, E, and Cheng, N. Comparison of radioimmunoassay and ELISA methods for detection of antibodies to chromatin components. Netherlands: N. p., 1983. Web.
Fowler, E, & Cheng, N. Comparison of radioimmunoassay and ELISA methods for detection of antibodies to chromatin components. Netherlands.
Fowler, E, and Cheng, N. 1983. "Comparison of radioimmunoassay and ELISA methods for detection of antibodies to chromatin components." Netherlands.
@misc{etde_5212687,
title = {Comparison of radioimmunoassay and ELISA methods for detection of antibodies to chromatin components}
author = {Fowler, E, and Cheng, N}
abstractNote = {A solid phase radioimmunoassay has been compared with an enzyme-linked immunosorbent assay (ELISA) for efficacy in measuring anti-chromatin antibodies. The low backgrounds achieved with the radioimmunoassay method produced a high signal-to-noise ratio and enabled detection of the human test antiserum at a dilution of 1:102,400. By contrast, the ELISA could detect the same antiserum only at a dilution of 1;3200 and above. The radioimmunoassay was consistently more sensitive than the ELSIA for detection of anti-chromatin antibodies in a number of human and mouse sera and ascites fluid containing a monoclonal antibody. Factors affecting sensitivity in both assays are discussed.}
journal = {J. Immunol. Methods; (Netherlands)}
volume = {62:3}
journal type = {AC}
place = {Netherlands}
year = {1983}
month = {Sep}
}