Abstract
Pathologic changes in renal glomeruli of mice with systemic murine lupus erythematosus were quantified using microfluorophotometry. Cryostat sections were taken from kidneys of affected mice, stained with fluorescein-conjugated anti-mouse immunoglobulin, and the extent of immune complex glomerulonephritis was determined. A subjective microscopic examination procedure, which has been used previously, was compared with quantitative microfluorophotometry and a close correlation between the results using each of the two methods was found. Since the microfluorometric procedure measures the total fluorescence per glomerulus, subjective microscopy must estimate that same quantity in a linear fashion. The present advance in measuring capability indicates good potential for rapid, quantitive measurements for further studies on systemic lupus erythematosus, and on other tissue sections stained with fluorescent antibodies.
Citation Formats
Jensen, R H, Greenspan, J S, Moore, D II, Talal, N, and Roubinian, J R.
Quantitative immunofluorescence microscopy of renal glomeruli from mice exhibiting murien lupus erythematosus.
Netherlands: N. p.,
1981.
Web.
Jensen, R H, Greenspan, J S, Moore, D II, Talal, N, & Roubinian, J R.
Quantitative immunofluorescence microscopy of renal glomeruli from mice exhibiting murien lupus erythematosus.
Netherlands.
Jensen, R H, Greenspan, J S, Moore, D II, Talal, N, and Roubinian, J R.
1981.
"Quantitative immunofluorescence microscopy of renal glomeruli from mice exhibiting murien lupus erythematosus."
Netherlands.
@misc{etde_5209454,
title = {Quantitative immunofluorescence microscopy of renal glomeruli from mice exhibiting murien lupus erythematosus}
author = {Jensen, R H, Greenspan, J S, Moore, D II, Talal, N, and Roubinian, J R}
abstractNote = {Pathologic changes in renal glomeruli of mice with systemic murine lupus erythematosus were quantified using microfluorophotometry. Cryostat sections were taken from kidneys of affected mice, stained with fluorescein-conjugated anti-mouse immunoglobulin, and the extent of immune complex glomerulonephritis was determined. A subjective microscopic examination procedure, which has been used previously, was compared with quantitative microfluorophotometry and a close correlation between the results using each of the two methods was found. Since the microfluorometric procedure measures the total fluorescence per glomerulus, subjective microscopy must estimate that same quantity in a linear fashion. The present advance in measuring capability indicates good potential for rapid, quantitive measurements for further studies on systemic lupus erythematosus, and on other tissue sections stained with fluorescent antibodies.}
journal = []
volume = {42}
journal type = {AC}
place = {Netherlands}
year = {1981}
month = {Jan}
}
title = {Quantitative immunofluorescence microscopy of renal glomeruli from mice exhibiting murien lupus erythematosus}
author = {Jensen, R H, Greenspan, J S, Moore, D II, Talal, N, and Roubinian, J R}
abstractNote = {Pathologic changes in renal glomeruli of mice with systemic murine lupus erythematosus were quantified using microfluorophotometry. Cryostat sections were taken from kidneys of affected mice, stained with fluorescein-conjugated anti-mouse immunoglobulin, and the extent of immune complex glomerulonephritis was determined. A subjective microscopic examination procedure, which has been used previously, was compared with quantitative microfluorophotometry and a close correlation between the results using each of the two methods was found. Since the microfluorometric procedure measures the total fluorescence per glomerulus, subjective microscopy must estimate that same quantity in a linear fashion. The present advance in measuring capability indicates good potential for rapid, quantitive measurements for further studies on systemic lupus erythematosus, and on other tissue sections stained with fluorescent antibodies.}
journal = []
volume = {42}
journal type = {AC}
place = {Netherlands}
year = {1981}
month = {Jan}
}