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Detection of endotoxins in radiopharmaceutical preparations. II. Comparison of the sensitivity of methods using the rabbit and the Limulus amoebocyte lysate for the detection of endotoxins

Journal Article:

Abstract

The rise of the rabbit internal temperature after i.v. injection of an endotoxin solution is proportional to concentration. Gelation of Limulus amoebocyte, when in presence of an endotoxin solution, is also related to concentration. We compared the sensitivity of these two methods. With our experimental procedure, the rabbit is sensitive to a 0.40 ng/mL solution and the Limulus amoebocyte lysate to a 0.14 ng/mL solution. The rabbit sensitivity increase is related to the per kilogramme injected volume, whereas sensitivity is not related to the volume to check in the case of the lysate.
Publication Date:
Jan 01, 1986
Product Type:
Journal Article
Reference Number:
EDB-86-168974
Resource Relation:
Journal Name: Int. J. Nucl. Med. Biol.; (United Kingdom); Journal Volume: 6
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ENDOTOXINS; DETECTION; RADIOPHARMACEUTICALS; CONTAMINATION; ESCHERICHIA COLI; FEVER; RABBITS; ANIMALS; ANTIGENS; BACTERIA; DRUGS; LABELLED COMPOUNDS; MAMMALS; MATERIALS; MICROORGANISMS; SYMPTOMS; TOXIC MATERIALS; TOXINS; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5205692
Research Organizations:
Commissariat a l'Energie Atomique, Gif-sur-Yvette, France
Country of Origin:
United Kingdom
Language:
French
Other Identifying Numbers:
Journal ID: CODEN: IJNMC
Submitting Site:
NLM
Size:
Pages: 471-476
Announcement Date:
Aug 01, 1986

Journal Article:

Citation Formats

Bruneau, J, Cohen, Y, Merlin, L, and Peysson, S. Detection of endotoxins in radiopharmaceutical preparations. II. Comparison of the sensitivity of methods using the rabbit and the Limulus amoebocyte lysate for the detection of endotoxins. United Kingdom: N. p., 1986. Web.
Bruneau, J, Cohen, Y, Merlin, L, & Peysson, S. Detection of endotoxins in radiopharmaceutical preparations. II. Comparison of the sensitivity of methods using the rabbit and the Limulus amoebocyte lysate for the detection of endotoxins. United Kingdom.
Bruneau, J, Cohen, Y, Merlin, L, and Peysson, S. 1986. "Detection of endotoxins in radiopharmaceutical preparations. II. Comparison of the sensitivity of methods using the rabbit and the Limulus amoebocyte lysate for the detection of endotoxins." United Kingdom.
@misc{etde_5205692,
title = {Detection of endotoxins in radiopharmaceutical preparations. II. Comparison of the sensitivity of methods using the rabbit and the Limulus amoebocyte lysate for the detection of endotoxins}
author = {Bruneau, J, Cohen, Y, Merlin, L, and Peysson, S}
abstractNote = {The rise of the rabbit internal temperature after i.v. injection of an endotoxin solution is proportional to concentration. Gelation of Limulus amoebocyte, when in presence of an endotoxin solution, is also related to concentration. We compared the sensitivity of these two methods. With our experimental procedure, the rabbit is sensitive to a 0.40 ng/mL solution and the Limulus amoebocyte lysate to a 0.14 ng/mL solution. The rabbit sensitivity increase is related to the per kilogramme injected volume, whereas sensitivity is not related to the volume to check in the case of the lysate.}
journal = {Int. J. Nucl. Med. Biol.; (United Kingdom)}
volume = {6}
journal type = {AC}
place = {United Kingdom}
year = {1986}
month = {Jan}
}