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Laser tweezers: spectroscopy of optically trapped micron-sized particles

Abstract

Information is often obtained about biological systems by analysis of single cells in the system. The optimum conditions for this analysis are when the cells are living and in their natural surroundings as they will be performing their normal functions and interactions. Analysis of cells can be difficult due to their mobility. Laser tweezing is a non contact method that can be employed to overcome this problem and provides a powerful tool in the analysis of functions and interactions at single cell level. In this investigation Raman spectra of a molecule of {beta} - carotene, dissolved in microdroplets of oil was obtained. The droplets were trapped using Nd-YAG beam and a low intensity Ar{sup +} beam was used to analyse the trapped particles. 2 refs., 5 figs.
Authors:
Kerr, K M; Livett, M K; Nugent, K W [1] 
  1. Melbourne Univ., Parkville, VIC (Australia). School of Physics
Publication Date:
Dec 31, 1996
Product Type:
Miscellaneous
Report Number:
INIS-AU-0003; CONF-9511295-
Reference Number:
SCA: 400102; PA: AIX-28:056397; EDB-97:119047; SN: 97001840870
Resource Relation:
Conference: 9. Australian conference on nuclear technique of analysis, Newcastle (Australia), 27-29 Nov 1995; Other Information: PBD: [1996]; Related Information: Is Part Of 9th Australian conference on nuclear techniques of analysis. Proceedings; PB: 186 p.
Subject:
40 CHEMISTRY; CAROTENOIDS; RAMAN SPECTROSCOPY; TRAPPING; ARGON IONS; BIOLOGICAL FUNCTIONS; BIOLOGICAL MATERIALS; EXPERIMENTAL DATA; INELASTIC SCATTERING; LASER RADIATION; NEODYMIUM IONS
OSTI ID:
520458
Research Organizations:
Australian Inst. of Nuclear Science and Engineering, Lucas Heights, NSW (Australia)
Country of Origin:
Australia
Language:
English
Other Identifying Numbers:
Other: ON: DE97638268; TRN: AU9716183056397
Availability:
INIS; OSTI as DE97638268
Submitting Site:
AUN
Size:
pp. 124-126
Announcement Date:

Citation Formats

Kerr, K M, Livett, M K, and Nugent, K W. Laser tweezers: spectroscopy of optically trapped micron-sized particles. Australia: N. p., 1996. Web.
Kerr, K M, Livett, M K, & Nugent, K W. Laser tweezers: spectroscopy of optically trapped micron-sized particles. Australia.
Kerr, K M, Livett, M K, and Nugent, K W. 1996. "Laser tweezers: spectroscopy of optically trapped micron-sized particles." Australia.
@misc{etde_520458,
title = {Laser tweezers: spectroscopy of optically trapped micron-sized particles}
author = {Kerr, K M, Livett, M K, and Nugent, K W}
abstractNote = {Information is often obtained about biological systems by analysis of single cells in the system. The optimum conditions for this analysis are when the cells are living and in their natural surroundings as they will be performing their normal functions and interactions. Analysis of cells can be difficult due to their mobility. Laser tweezing is a non contact method that can be employed to overcome this problem and provides a powerful tool in the analysis of functions and interactions at single cell level. In this investigation Raman spectra of a molecule of {beta} - carotene, dissolved in microdroplets of oil was obtained. The droplets were trapped using Nd-YAG beam and a low intensity Ar{sup +} beam was used to analyse the trapped particles. 2 refs., 5 figs.}
place = {Australia}
year = {1996}
month = {Dec}
}