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Development of a test system for the determination of biodegradability in surface waters; Entwicklung eines Testsystems fuer die Pruefung des biologischen Abbaus in Oberflaechengewaessern

Abstract

The study presented here describes the development of a laboratory test system for the determination of aerobic biodegradability of substances at low concentrations in surface water. It was aimed to prepare a draft guideline for a biodegradation simulation test according to OECD format. The experimental approach was based on a literature study conducted within the frame of this project. Further useful information on the possible test design was derived from the German BBA guideline 5-1. Natural water and sediments were collected. Radiolabelled Lindane or 4-Nitrophenol was added. The test vessels (reactors) were aerated and incubated under controlled conditions for up to 92 days. The results showed biological stability of the sediment/water systems even without addition of nutrients and adherence to non-reducing conditions. Mineralisation of 4-Nitrophenol was influenced by the sediment type, the method of aeration and temperature. Factors affecting the mineralisation of Lindane were the method of application and again, the sediment type and temperature. Considerable amounts of the radioactivity were bound to the sediment and were to a large extent unextractable. The potential of a reactor to mineralise a test substance could not be correlated with the biological parameters measured. (orig.) [Deutsch] Die vorliegende Studie beschreibt die Entwicklung eines  More>>
Publication Date:
Apr 01, 1997
Product Type:
Technical Report
Report Number:
UBA-FB-97-031
Reference Number:
SCA: 540320; PA: DEN-97:0FC661; EDB-97:120841; SN: 97001843680
Resource Relation:
Other Information: PBD: Apr 1997; Related Information: Umweltbundesamt. Texte, v. 13/97
Subject:
54 ENVIRONMENTAL SCIENCES; LINDANE; BIODEGRADATION; NITROPHENOL; WATER POLLUTION; SEDIMENTS; TRACER TECHNIQUES; ENZYMES; MEASURING METHODS; AEROBIC CONDITIONS
Sponsoring Organizations:
Bundesministerium fuer Umwelt, Naturschutz und Reaktorsicherheit, Bonn (Germany)
OSTI ID:
520348
Research Organizations:
Umweltbundesamt, Berlin (Germany); ECT Oekotoxikologie GmbH, Floersheim (Germany)
Country of Origin:
Germany
Language:
German;English
Other Identifying Numbers:
Journal ID: ISSN 0722-186X; Other: ON: DE97768896; CNN: Contract UFOPLAN 10603120/02; TRN: DE97FC661
Availability:
INIS; OSTI as DE97768896
Submitting Site:
DEN
Size:
117 p.
Announcement Date:

Citation Formats

Kalsch, W, Knacker, T, Robertz, M, and Schallnass, H J. Development of a test system for the determination of biodegradability in surface waters; Entwicklung eines Testsystems fuer die Pruefung des biologischen Abbaus in Oberflaechengewaessern. Germany: N. p., 1997. Web.
Kalsch, W, Knacker, T, Robertz, M, & Schallnass, H J. Development of a test system for the determination of biodegradability in surface waters; Entwicklung eines Testsystems fuer die Pruefung des biologischen Abbaus in Oberflaechengewaessern. Germany.
Kalsch, W, Knacker, T, Robertz, M, and Schallnass, H J. 1997. "Development of a test system for the determination of biodegradability in surface waters; Entwicklung eines Testsystems fuer die Pruefung des biologischen Abbaus in Oberflaechengewaessern." Germany.
@misc{etde_520348,
title = {Development of a test system for the determination of biodegradability in surface waters; Entwicklung eines Testsystems fuer die Pruefung des biologischen Abbaus in Oberflaechengewaessern}
author = {Kalsch, W, Knacker, T, Robertz, M, and Schallnass, H J}
abstractNote = {The study presented here describes the development of a laboratory test system for the determination of aerobic biodegradability of substances at low concentrations in surface water. It was aimed to prepare a draft guideline for a biodegradation simulation test according to OECD format. The experimental approach was based on a literature study conducted within the frame of this project. Further useful information on the possible test design was derived from the German BBA guideline 5-1. Natural water and sediments were collected. Radiolabelled Lindane or 4-Nitrophenol was added. The test vessels (reactors) were aerated and incubated under controlled conditions for up to 92 days. The results showed biological stability of the sediment/water systems even without addition of nutrients and adherence to non-reducing conditions. Mineralisation of 4-Nitrophenol was influenced by the sediment type, the method of aeration and temperature. Factors affecting the mineralisation of Lindane were the method of application and again, the sediment type and temperature. Considerable amounts of the radioactivity were bound to the sediment and were to a large extent unextractable. The potential of a reactor to mineralise a test substance could not be correlated with the biological parameters measured. (orig.) [Deutsch] Die vorliegende Studie beschreibt die Entwicklung eines Labortestverfahrens zur Pruefung des aeroben Abbaus niedrig konzentrierter Stoffe in Oberflaechengewaessern. Dabei war es ein Ziel, das Verfahren so weit abzusichern, dass ein Entwurf fuer eine Pruefrichtlinie als Simulationstest im Format der OECD-Richtlinien abgefasst werden konnte. Grundlage fuer die Konzeption war eine zuvoerderst durchgefuehrte Literaturstudie. Hinweise auf ein moegliches Testdesign ergaben sich auch aus der BBA-Richtlinie 5-1. Wasser und Sediment wurden der Natur entnommen und nach Zugabe der radioaktiven Pruefsubstanz Lindan oder 4-Nitrophenol in einem beluefteten Gefaess unter kontrollierten Bedingungen bis zu 92 Tage lang inkubiert. Die Ergebnisse zeigten, dass die Wasser/Sediment-Systeme auch ueber diesen langen Zeitraum ohne Zufuetterung biologisch stabil waren und nichtreduzierende Bedingungen auch im Sediment erhalten blieben. Die Mineralisierung von 4-Nitrophenol wurde vom Sedimenttyp, der Belueftungsart und der Temperatur beeinflusst, die von Lindan von der Applikationsart und ebenfalls vom Sedimenttyp und der Temperatur. Wesentliche Anteile der Radioaktivitaet wurden ans Sediment gebunden und waren zum grossen Teil nicht extrahierbar. Es wird schliesslich diskutiert, dass das Potential eines Sediments, Stoffe zu minimalisieren, nicht mit den gewaehlten biologischen Kenngroessen klassifiziert werden kann. Daraus koennen Probleme bei der Uebertragbarkeit der Ergebnisse auf neue Standorte erwachsen. (orig.)}
place = {Germany}
year = {1997}
month = {Apr}
}