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Radiolabeling and biotinylation of internalizing monoclonal antibody chimeric BR96: Potential use of extracorporeal immunoadsorption with enhanced tumor radioactivity retention of Iodine, Indium and Rhenium

Abstract

In this thesis, methodology of radiolabeling and simultaneous biotinylation for internalizing monoclonal antibody chimeric BR96 have been investigated by using three element groups of potential therapeutic radionuclides iodine, indium and rhenium, and their different labeling methods. The biodistribution and kinetics of biotinylated and radiolabeled chiBR96 have been studied in colon carcinoma isografted rats. The potential use of ECIA, based on the biotin-avidin concept, has been evaluated and compared with the approach of avidin `chase` in the same animal tumor model with respect to an enhancement of tumor-to-normal tissue (T/N) activity ratio. 131 refs.
Authors:
Publication Date:
Jan 01, 1997
Product Type:
Technical Report
Report Number:
LUNFD6-NFRA-1032; LUMEDW-MERI-1032.
Reference Number:
SCA: 550604; PA: AIX-28:059316; EDB-97:121343; SN: 97001844394
Resource Relation:
Other Information: TH: Diss. (FD).; PBD: Jan 1997
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; LABELLED COMPOUNDS; MONOCLONAL ANTIBODIES; LABELLING; BIOTIN; CHIMERAS; EXPERIMENTAL DATA; INDIUM 111; IODINE 125; NEOPLASMS; RADIOIMMUNOTHERAPY; RHENIUM 188
OSTI ID:
519601
Research Organizations:
Lund Univ. (Sweden). Dept. of Radiation Physics
Country of Origin:
Sweden
Language:
English
Other Identifying Numbers:
Other: ON: DE97640048; ISBN 91-628-2329-9; TRN: SE9700166059316
Availability:
INIS; OSTI as DE97640048
Submitting Site:
SWDN
Size:
55 p.
Announcement Date:

Citation Formats

Chen, JianQing. Radiolabeling and biotinylation of internalizing monoclonal antibody chimeric BR96: Potential use of extracorporeal immunoadsorption with enhanced tumor radioactivity retention of Iodine, Indium and Rhenium. Sweden: N. p., 1997. Web.
Chen, JianQing. Radiolabeling and biotinylation of internalizing monoclonal antibody chimeric BR96: Potential use of extracorporeal immunoadsorption with enhanced tumor radioactivity retention of Iodine, Indium and Rhenium. Sweden.
Chen, JianQing. 1997. "Radiolabeling and biotinylation of internalizing monoclonal antibody chimeric BR96: Potential use of extracorporeal immunoadsorption with enhanced tumor radioactivity retention of Iodine, Indium and Rhenium." Sweden.
@misc{etde_519601,
title = {Radiolabeling and biotinylation of internalizing monoclonal antibody chimeric BR96: Potential use of extracorporeal immunoadsorption with enhanced tumor radioactivity retention of Iodine, Indium and Rhenium}
author = {Chen, JianQing}
abstractNote = {In this thesis, methodology of radiolabeling and simultaneous biotinylation for internalizing monoclonal antibody chimeric BR96 have been investigated by using three element groups of potential therapeutic radionuclides iodine, indium and rhenium, and their different labeling methods. The biodistribution and kinetics of biotinylated and radiolabeled chiBR96 have been studied in colon carcinoma isografted rats. The potential use of ECIA, based on the biotin-avidin concept, has been evaluated and compared with the approach of avidin `chase` in the same animal tumor model with respect to an enhancement of tumor-to-normal tissue (T/N) activity ratio. 131 refs.}
place = {Sweden}
year = {1997}
month = {Jan}
}