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In vitro selection of induced mutants to salt-tolerance: Inducible gene regulation for salt tolerance

Abstract

A selection protocol to obtain salt tolerant calli, followed by regeneration and progeny-test of the regenerated plants for salt tolerance in rice was investigated. Callus cultures were initiated from salt-sensitive US elite rice lines and cv. `Pokkali`. Salt-tolerant cell lines were selected from these by a single step selection procedure. The selected salt-tolerant lines grew well on medium with {+-} 0.5% or 1% NaCl, while the parent lines occasionally survived, but did not grow at these salt concentrations. Plants were regenerated from these cell lines through different passages on medium containing salt. Seed was collected from the regenerated plants and salt tolerance of R2 seedlings was compared with those regenerated without salt selection. Salt-tolerance was measured by survival and productive growth of newly germinated seedlings in Hoagland solution with 0.3% and 0.5% NaCl for 4 weeks. Heritable improvement in salt tolerance was obtained in R2 seedlings from one plant regenerated after 5 months selection. Survival and growth of these seedlings was equivalent to that from `Pokkali` seedlings. These results show that cellular tolerance can provide salt-tolerance in rice plants. (author). 6 refs, 2 tabs.
Authors:
Winicov, I [1] 
  1. Department of Microbiology and Biochemistry, Univ. of Nevada-Reno, Reno, NV (United States)
Publication Date:
Jul 01, 1997
Product Type:
Conference
Report Number:
IAEA-312.D2.RC.545; CONF-9604223-
Reference Number:
SCA: 553002; PA: AIX-28:058880; EDB-97:121384; SN: 97001844123
Resource Relation:
Conference: 2. FAO/IAEA research coordination meeting on in vitro techniques for selection of radiation-induced mutants adapted to adverse environmental conditions, Cairo (Egypt), 15-19 Apr 1996; Other Information: PBD: Jul 1997; Related Information: Is Part Of Report of second FAO/IAEA research coordination meeting on in vitro techniques for selection of radiation-induced mutants adapted to adverse environmental conditions. Working material; PB: 85 p.
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; RICE; RADIATION INDUCED MUTANTS; SALINITY; TOLERANCE; BIOLOGICAL RADIATION EFFECTS; IN VITRO; MUTAGEN SCREENING; PROGENY; SEEDLINGS
OSTI ID:
519230
Research Organizations:
Joint FAO/IAEA Div. of Nuclear Techniques in Food and Agriculture, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: ON: DE97639792; TRN: XA9744861058880
Availability:
INIS; OSTI as DE97639792
Submitting Site:
INIS
Size:
pp. 59-62
Announcement Date:

Citation Formats

Winicov, I. In vitro selection of induced mutants to salt-tolerance: Inducible gene regulation for salt tolerance. IAEA: N. p., 1997. Web.
Winicov, I. In vitro selection of induced mutants to salt-tolerance: Inducible gene regulation for salt tolerance. IAEA.
Winicov, I. 1997. "In vitro selection of induced mutants to salt-tolerance: Inducible gene regulation for salt tolerance." IAEA.
@misc{etde_519230,
title = {In vitro selection of induced mutants to salt-tolerance: Inducible gene regulation for salt tolerance}
author = {Winicov, I}
abstractNote = {A selection protocol to obtain salt tolerant calli, followed by regeneration and progeny-test of the regenerated plants for salt tolerance in rice was investigated. Callus cultures were initiated from salt-sensitive US elite rice lines and cv. `Pokkali`. Salt-tolerant cell lines were selected from these by a single step selection procedure. The selected salt-tolerant lines grew well on medium with {+-} 0.5% or 1% NaCl, while the parent lines occasionally survived, but did not grow at these salt concentrations. Plants were regenerated from these cell lines through different passages on medium containing salt. Seed was collected from the regenerated plants and salt tolerance of R2 seedlings was compared with those regenerated without salt selection. Salt-tolerance was measured by survival and productive growth of newly germinated seedlings in Hoagland solution with 0.3% and 0.5% NaCl for 4 weeks. Heritable improvement in salt tolerance was obtained in R2 seedlings from one plant regenerated after 5 months selection. Survival and growth of these seedlings was equivalent to that from `Pokkali` seedlings. These results show that cellular tolerance can provide salt-tolerance in rice plants. (author). 6 refs, 2 tabs.}
place = {IAEA}
year = {1997}
month = {Jul}
}