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In vitro induction of variation through radiation for late blight resistance and heat tolerance in potato

Abstract

In vitro plants were obtained from nodal sections of sprouts of cvs. `Kufri Jyoti` and `Kufri Chandramukhi` of potato cultured on MS medium with 3% sucrose. Callus from leaves of in vitro cultured plantlets was induced on modified Linsmaier and Skoog medium supplemented with 5 mg/1 NAA. The obtained shoots and calli were irradiated with 20 and 40 Gy gamma rays. Irradiatied shoots were transferred to MS medium with 8% sucrose for multiplication, and then to MS medium with 8% sucrose and 10 mg/1 BAP to induce microtuber formation, which gave on average 1.3 microtubers per plant. The microtubers were planted in pots and variation was observed in plant morphology and tuber characters. To study variation for late blight resistance, irradiated calli were kept on Gamborg B-5 medium with culture filtrate of Phytophthora infestans. To induce variation for heat tolerance, in vitro shoots from irradiated material were mass-propagated and allowed to produce microtubers at high temperature. (author). 3 refs, 3 tabs.
Authors:
Minocha, J L; Das, A; Gopal, J; Gosal, S S [1] 
  1. Biotechnology Centre, Punjab Agricultural Univ., Ludhiana, Punjab (India)
Publication Date:
Jul 01, 1997
Product Type:
Conference
Report Number:
IAEA-312.D2.RC.545; CONF-9604223-
Reference Number:
SCA: 553002; PA: AIX-28:058875; EDB-97:121375; SN: 97001844118
Resource Relation:
Conference: 2. FAO/IAEA research coordination meeting on in vitro techniques for selection of radiation-induced mutants adapted to adverse environmental conditions, Cairo (Egypt), 15-19 Apr 1996; Other Information: PBD: Jul 1997; Related Information: Is Part Of Report of second FAO/IAEA research coordination meeting on in vitro techniques for selection of radiation-induced mutants adapted to adverse environmental conditions. Working material; PB: 85 p.
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; SOLANUM TUBEROSUM; DISEASE RESISTANCE; RADIATION INDUCED MUTANTS; IN VITRO; POTATOES; PRODUCTIVITY; TISSUE CULTURES
OSTI ID:
519225
Research Organizations:
Joint FAO/IAEA Div. of Nuclear Techniques in Food and Agriculture, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
Other: ON: DE97639792; TRN: XA9744856058875
Availability:
INIS; OSTI as DE97639792
Submitting Site:
INIS
Size:
pp. 29-33
Announcement Date:

Citation Formats

Minocha, J L, Das, A, Gopal, J, and Gosal, S S. In vitro induction of variation through radiation for late blight resistance and heat tolerance in potato. IAEA: N. p., 1997. Web.
Minocha, J L, Das, A, Gopal, J, & Gosal, S S. In vitro induction of variation through radiation for late blight resistance and heat tolerance in potato. IAEA.
Minocha, J L, Das, A, Gopal, J, and Gosal, S S. 1997. "In vitro induction of variation through radiation for late blight resistance and heat tolerance in potato." IAEA.
@misc{etde_519225,
title = {In vitro induction of variation through radiation for late blight resistance and heat tolerance in potato}
author = {Minocha, J L, Das, A, Gopal, J, and Gosal, S S}
abstractNote = {In vitro plants were obtained from nodal sections of sprouts of cvs. `Kufri Jyoti` and `Kufri Chandramukhi` of potato cultured on MS medium with 3% sucrose. Callus from leaves of in vitro cultured plantlets was induced on modified Linsmaier and Skoog medium supplemented with 5 mg/1 NAA. The obtained shoots and calli were irradiated with 20 and 40 Gy gamma rays. Irradiatied shoots were transferred to MS medium with 8% sucrose for multiplication, and then to MS medium with 8% sucrose and 10 mg/1 BAP to induce microtuber formation, which gave on average 1.3 microtubers per plant. The microtubers were planted in pots and variation was observed in plant morphology and tuber characters. To study variation for late blight resistance, irradiated calli were kept on Gamborg B-5 medium with culture filtrate of Phytophthora infestans. To induce variation for heat tolerance, in vitro shoots from irradiated material were mass-propagated and allowed to produce microtubers at high temperature. (author). 3 refs, 3 tabs.}
place = {IAEA}
year = {1997}
month = {Jul}
}