Abstract
Total body protein turnover was studied in six elderly patients. During the study they were fed by continuous infusion of a liquid formula through a nasogastric tube. L-(1-/sup 14/C)leucine and (/sup 15/N)-glycine were infused at a constant rate for 30 h. The labelled glycine was infused into the intragastric line; the labelled leucine was given either by this route or intravenously. The specific radioactivity of free leucine in plasma and the rate of output of /sup 14/CO/sub 2/ in expired air both reached a plateau at 10 h, and remained constant until the end of the infusion at 30 h. The /sup 15/N abundance in urinary urea and total N was very similar. In neither was a plateau reached by 30 h but in four out of the six patients the abundance in urinary NH/sub 4//sup +/ had attained a plateau by the end of the infusion. Flux rates and rates of protein synthesis were calculated in four ways and a comparison of methods was used to examine the validity of the assumptions on which the different methods depended. The results suggest that the rate of protein turnover is reduced in the elderly, compared with younger subjects.
Citation Formats
Golden, M H.N., and Waterlow, J C.
Total protein synthesis in elderly people; a comparison of results with (/sup 15/N)glycine and (/sup 14/C)leucine.
United Kingdom: N. p.,
1977.
Web.
Golden, M H.N., & Waterlow, J C.
Total protein synthesis in elderly people; a comparison of results with (/sup 15/N)glycine and (/sup 14/C)leucine.
United Kingdom.
Golden, M H.N., and Waterlow, J C.
1977.
"Total protein synthesis in elderly people; a comparison of results with (/sup 15/N)glycine and (/sup 14/C)leucine."
United Kingdom.
@misc{etde_5179908,
title = {Total protein synthesis in elderly people; a comparison of results with (/sup 15/N)glycine and (/sup 14/C)leucine}
author = {Golden, M H.N., and Waterlow, J C}
abstractNote = {Total body protein turnover was studied in six elderly patients. During the study they were fed by continuous infusion of a liquid formula through a nasogastric tube. L-(1-/sup 14/C)leucine and (/sup 15/N)-glycine were infused at a constant rate for 30 h. The labelled glycine was infused into the intragastric line; the labelled leucine was given either by this route or intravenously. The specific radioactivity of free leucine in plasma and the rate of output of /sup 14/CO/sub 2/ in expired air both reached a plateau at 10 h, and remained constant until the end of the infusion at 30 h. The /sup 15/N abundance in urinary urea and total N was very similar. In neither was a plateau reached by 30 h but in four out of the six patients the abundance in urinary NH/sub 4//sup +/ had attained a plateau by the end of the infusion. Flux rates and rates of protein synthesis were calculated in four ways and a comparison of methods was used to examine the validity of the assumptions on which the different methods depended. The results suggest that the rate of protein turnover is reduced in the elderly, compared with younger subjects.}
journal = []
volume = {53:3}
journal type = {AC}
place = {United Kingdom}
year = {1977}
month = {Sep}
}
title = {Total protein synthesis in elderly people; a comparison of results with (/sup 15/N)glycine and (/sup 14/C)leucine}
author = {Golden, M H.N., and Waterlow, J C}
abstractNote = {Total body protein turnover was studied in six elderly patients. During the study they were fed by continuous infusion of a liquid formula through a nasogastric tube. L-(1-/sup 14/C)leucine and (/sup 15/N)-glycine were infused at a constant rate for 30 h. The labelled glycine was infused into the intragastric line; the labelled leucine was given either by this route or intravenously. The specific radioactivity of free leucine in plasma and the rate of output of /sup 14/CO/sub 2/ in expired air both reached a plateau at 10 h, and remained constant until the end of the infusion at 30 h. The /sup 15/N abundance in urinary urea and total N was very similar. In neither was a plateau reached by 30 h but in four out of the six patients the abundance in urinary NH/sub 4//sup +/ had attained a plateau by the end of the infusion. Flux rates and rates of protein synthesis were calculated in four ways and a comparison of methods was used to examine the validity of the assumptions on which the different methods depended. The results suggest that the rate of protein turnover is reduced in the elderly, compared with younger subjects.}
journal = []
volume = {53:3}
journal type = {AC}
place = {United Kingdom}
year = {1977}
month = {Sep}
}