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Inhibition of time-dependent enhancement of amino acid transport by leukemic leukocytes: a possible index of the sensitivity of cells to drugs

Journal Article:

Abstract

Leukemic leukocytes increase their rates of alpha aminoisobutyric acid (AIB) accumulation when incubated for prolonged periods in amino acid deficient media. The time-dependent increase was prevented by concurrent exposure of cells to cycloheximide or actinomycin D in vitro. In addition, the increase in AIB uptake was not present in leukemic blasts studied in vitro when the cells were obtained from subjects with acute myeloblastic leukemia who had received antileukemic therapy. Cortisol added to cell suspensions in vitro inhibited the development of time-dependent increases in AIB uptake in lymphoid cells, but accentuated the process slightly in myeloblasts. Cortisol administered to a subject with CLL by infusion reduced the time-dependent increase in AIB uptake by CLL cells subsequently studied in vitro. These data indicate that the time-dependent increase in AIB uptake may be a means of testing the sensitivity of leukemic cells to drugs.
Publication Date:
Jan 01, 1975
Product Type:
Journal Article
Reference Number:
ERA-03-036067; EDB-78-070735
Resource Relation:
Journal Name: Blood Cells; (Germany, Federal Republic of); Journal Volume: 1
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 59 BASIC BIOLOGICAL SCIENCES; LEUKEMIA; CHEMOTHERAPY; ACTINOMYCIN; AMINO ACIDS; BLOOD; CYCLOHEXIMIDE; DIAGNOSTIC TECHNIQUES; DRUGS; HYDROCORTISONE; INHIBITION; LEUKOCYTES; NEOPLASMS; SENSITIVITY; TIME DEPENDENCE; UPTAKE; ADRENAL HORMONES; ANTIBIOTICS; ANTIMITOTIC DRUGS; BIOLOGICAL MATERIALS; BLOOD CELLS; BODY FLUIDS; CARBOXYLIC ACIDS; CORTICOSTEROIDS; DISEASES; FUNGICIDES; GLUCOCORTICOIDS; HEMIC DISEASES; HORMONES; HYDROXY COMPOUNDS; KETONES; ORGANIC ACIDS; ORGANIC COMPOUNDS; PESTICIDES; PREGNANES; STEROID HORMONES; STEROIDS; THERAPY; 550600* - Medicine; 550900 - Pathology
OSTI ID:
5142886
Research Organizations:
Univ. of Rochester School of Medicine, NY
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BLCED
Submitting Site:
TIC
Size:
Pages: 139-146
Announcement Date:

Journal Article:

Citation Formats

Frengley, P A, Peck, W A, and Lichtman, M A. Inhibition of time-dependent enhancement of amino acid transport by leukemic leukocytes: a possible index of the sensitivity of cells to drugs. Germany: N. p., 1975. Web.
Frengley, P A, Peck, W A, & Lichtman, M A. Inhibition of time-dependent enhancement of amino acid transport by leukemic leukocytes: a possible index of the sensitivity of cells to drugs. Germany.
Frengley, P A, Peck, W A, and Lichtman, M A. 1975. "Inhibition of time-dependent enhancement of amino acid transport by leukemic leukocytes: a possible index of the sensitivity of cells to drugs." Germany.
@misc{etde_5142886,
title = {Inhibition of time-dependent enhancement of amino acid transport by leukemic leukocytes: a possible index of the sensitivity of cells to drugs}
author = {Frengley, P A, Peck, W A, and Lichtman, M A}
abstractNote = {Leukemic leukocytes increase their rates of alpha aminoisobutyric acid (AIB) accumulation when incubated for prolonged periods in amino acid deficient media. The time-dependent increase was prevented by concurrent exposure of cells to cycloheximide or actinomycin D in vitro. In addition, the increase in AIB uptake was not present in leukemic blasts studied in vitro when the cells were obtained from subjects with acute myeloblastic leukemia who had received antileukemic therapy. Cortisol added to cell suspensions in vitro inhibited the development of time-dependent increases in AIB uptake in lymphoid cells, but accentuated the process slightly in myeloblasts. Cortisol administered to a subject with CLL by infusion reduced the time-dependent increase in AIB uptake by CLL cells subsequently studied in vitro. These data indicate that the time-dependent increase in AIB uptake may be a means of testing the sensitivity of leukemic cells to drugs.}
journal = {Blood Cells; (Germany, Federal Republic of)}
volume = {1}
journal type = {AC}
place = {Germany}
year = {1975}
month = {Jan}
}