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/sup 1/H-NMR urinalysis. Simultaneous screening of inborn errors of metabolism of amino acid and organic acid disorders

Abstract

In an effort to examine the usefulness of /sup 1/H-nuclear magnetic resonance (NMR) urinalysis in the diagnosis of congenital metabolic disorders, 70 kinds of urinary metabolites were analysed in relation to the diagnosis of inborn errors of amino acid and organic acid disorders. Homogated decoupling (HMG) method failed to analyze six metabolites within the undetectable range. When non-decoupling method (NON), in which the materials are dissolved in dimethyl sulfoxide, was used, the identification of signals became possible. The combination of HMG and NON methods was, therefore, considered to identify all of the metabolites. When the urine samples, which were obtained from patients with hyperglycerolemia, hyperornithinemia, glutaric acidemia type II, or glycerol kinase deficiency, were analysed by using both HMG and NON methods, abnormally increased urinary metabolites were detected. /sup 1/H-NMR urinalysis, if used in the combination of HMG and NON methods, may allow simultanenous screening of inborn errors of metabolism of amino acid and organic acid disorders. (Namekawa, K.).
Publication Date:
Feb 01, 1988
Product Type:
Journal Article
Reference Number:
JPN-88-074275; EDB-88-107062
Resource Relation:
Journal Name: Rinsho Byori; (Japan); Journal Volume: 36:2
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; METABOLIC DISEASES; NUCLEAR MAGNETIC RESONANCE; URINE; ALANINES; CHEMICAL SHIFT; CREATINE; EXCRETION; GLYCINE; NMR SPECTRA; PATIENTS; AMINO ACIDS; BIOLOGICAL MATERIALS; BIOLOGICAL WASTES; BODY FLUIDS; CARBOXYLIC ACIDS; CLEARANCE; DISEASES; MAGNETIC RESONANCE; MATERIALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; RESONANCE; SPECTRA; WASTES; 550600* - Medicine
OSTI ID:
5138170
Research Organizations:
Saitama Children's Medical Center (Japan)
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Journal ID: CODEN: RBYOA
Submitting Site:
JPN
Size:
Pages: 182-190
Announcement Date:
Jun 01, 1988

Citation Formats

Yamamoto, Hideaki, and Yamaguchi, Shuichi. /sup 1/H-NMR urinalysis. Simultaneous screening of inborn errors of metabolism of amino acid and organic acid disorders. Japan: N. p., 1988. Web.
Yamamoto, Hideaki, & Yamaguchi, Shuichi. /sup 1/H-NMR urinalysis. Simultaneous screening of inborn errors of metabolism of amino acid and organic acid disorders. Japan.
Yamamoto, Hideaki, and Yamaguchi, Shuichi. 1988. "/sup 1/H-NMR urinalysis. Simultaneous screening of inborn errors of metabolism of amino acid and organic acid disorders." Japan.
@misc{etde_5138170,
title = {/sup 1/H-NMR urinalysis. Simultaneous screening of inborn errors of metabolism of amino acid and organic acid disorders}
author = {Yamamoto, Hideaki, and Yamaguchi, Shuichi}
abstractNote = {In an effort to examine the usefulness of /sup 1/H-nuclear magnetic resonance (NMR) urinalysis in the diagnosis of congenital metabolic disorders, 70 kinds of urinary metabolites were analysed in relation to the diagnosis of inborn errors of amino acid and organic acid disorders. Homogated decoupling (HMG) method failed to analyze six metabolites within the undetectable range. When non-decoupling method (NON), in which the materials are dissolved in dimethyl sulfoxide, was used, the identification of signals became possible. The combination of HMG and NON methods was, therefore, considered to identify all of the metabolites. When the urine samples, which were obtained from patients with hyperglycerolemia, hyperornithinemia, glutaric acidemia type II, or glycerol kinase deficiency, were analysed by using both HMG and NON methods, abnormally increased urinary metabolites were detected. /sup 1/H-NMR urinalysis, if used in the combination of HMG and NON methods, may allow simultanenous screening of inborn errors of metabolism of amino acid and organic acid disorders. (Namekawa, K.).}
journal = []
volume = {36:2}
journal type = {AC}
place = {Japan}
year = {1988}
month = {Feb}
}