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Enrichment of measles virus-like RNA in the nucleocapsid fraction isolated from subacute sclerosing panencephalitis brains

Abstract

A procedure has been developed which facilitates the detection of measles virus RNA sequences in human brains. The procedure involves isolating subviral components (nucleocapsids) from brain tissues prior to RNA purification, followed by hybridization of these RNAs to cDNA synthesized from measles virus 50 S RNA template. Using these techniques we were able to obtain an RNA fraction which was manyfold enriched in measles virus-specific RNA, relative to unfractionated subacute sclerosing panencephalitis (SSPE) brain RNAs. 70-100% of the measles virus-specific RNA present in these SSPE brain samples were recovered in this enriched fraction.
Authors:
Bedows, E; Payne, F E; [1]  Kohne, D E; [2]  Tourtellotte, W W [3] 
  1. Michigan Univ., Ann Arbor (USA). School of Public Health
  2. Center for Neurologic Study, San Diego, CA, USA
  3. Neurology Service, V.A. Wadsworth Hospital Center, Los Angeles, CA, USA
Publication Date:
Feb 01, 1982
Product Type:
Journal Article
Reference Number:
AIX-13-684218; EDB-82-146198
Resource Relation:
Journal Name: J. Virol. Methods; (Netherlands); Journal Volume: 4:1
Subject:
59 BASIC BIOLOGICAL SCIENCES; MEASLES VIRUS; SEPARATION PROCESSES; RNA; BRAIN; DECOMPOSITION; DISEASES; ENCEPHALITIS; HYBRIDIZATION; INCUBATION; PURIFICATION; TRITIUM COMPOUNDS; URIDINE; AZINES; BODY; CENTRAL NERVOUS SYSTEM; CHEMICAL REACTIONS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INFECTIOUS DISEASES; LABELLED COMPOUNDS; MICROORGANISMS; NERVOUS SYSTEM; NERVOUS SYSTEM DISEASES; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PARASITES; PYRIMIDINES; RIBOSIDES; URACILS; VIRAL DISEASES; VIRUSES; 550201* - Biochemistry- Tracer Techniques
OSTI ID:
5136060
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JVMED
Submitting Site:
HEDB
Size:
Pages: 37-43
Announcement Date:
Jun 01, 1982

Citation Formats

Bedows, E, Payne, F E, Kohne, D E, and Tourtellotte, W W. Enrichment of measles virus-like RNA in the nucleocapsid fraction isolated from subacute sclerosing panencephalitis brains. Netherlands: N. p., 1982. Web.
Bedows, E, Payne, F E, Kohne, D E, & Tourtellotte, W W. Enrichment of measles virus-like RNA in the nucleocapsid fraction isolated from subacute sclerosing panencephalitis brains. Netherlands.
Bedows, E, Payne, F E, Kohne, D E, and Tourtellotte, W W. 1982. "Enrichment of measles virus-like RNA in the nucleocapsid fraction isolated from subacute sclerosing panencephalitis brains." Netherlands.
@misc{etde_5136060,
title = {Enrichment of measles virus-like RNA in the nucleocapsid fraction isolated from subacute sclerosing panencephalitis brains}
author = {Bedows, E, Payne, F E, Kohne, D E, and Tourtellotte, W W}
abstractNote = {A procedure has been developed which facilitates the detection of measles virus RNA sequences in human brains. The procedure involves isolating subviral components (nucleocapsids) from brain tissues prior to RNA purification, followed by hybridization of these RNAs to cDNA synthesized from measles virus 50 S RNA template. Using these techniques we were able to obtain an RNA fraction which was manyfold enriched in measles virus-specific RNA, relative to unfractionated subacute sclerosing panencephalitis (SSPE) brain RNAs. 70-100% of the measles virus-specific RNA present in these SSPE brain samples were recovered in this enriched fraction.}
journal = []
volume = {4:1}
journal type = {AC}
place = {Netherlands}
year = {1982}
month = {Feb}
}