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Quantitative assay for the measurement of immune responses directed against the human placenta

Journal Article:

Abstract

A quantitative in vitro immune assay based on the classical chromium release assay has been developed to detect immune responses directed against alien antigens expressed by the developing foetus and present on the maternal-facing surface of the human placenta. A plasma membrane fraction from the surface of the placenta was prepared and the vesicles thus formed were radiolabelled with /sup 51/Cr. The /sup 51/Cr-labelled vesicles, by various criteria, were found to be suitable for use as targets in a release assay. Further, by means of experimentally immunised animals, the target membranes were shown to be capable of detecting both cellular and humoral anti-placental activity.
Authors:
Davies, M; Sutcliffe, R G [1] 
  1. Glasgow Univ. (UK)
Publication Date:
Feb 12, 1982
Product Type:
Journal Article
Reference Number:
AIX-13-677870; EDB-82-146285
Resource Relation:
Journal Name: J. Immunol. Methods; (Netherlands); Journal Volume: 48:3
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; IMMUNE REACTIONS; RADIOASSAY; PLACENTA; ANTIGENS; CHROMIUM 51; IN VITRO; LABELLED COMPOUNDS; MAN; RADIONUCLIDE KINETICS; SENSITIVITY; UPTAKE; ANIMALS; BETA DECAY RADIOISOTOPES; CHROMIUM ISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; FETAL MEMBRANES; INTERMEDIATE MASS NUCLEI; ISOTOPES; MAMMALS; MEMBRANES; NUCLEI; PRIMATES; RADIOISOTOPES; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
5135294
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JIMMB
Submitting Site:
HEDB
Size:
Pages: 373-383
Announcement Date:

Journal Article:

Citation Formats

Davies, M, and Sutcliffe, R G. Quantitative assay for the measurement of immune responses directed against the human placenta. Netherlands: N. p., 1982. Web.
Davies, M, & Sutcliffe, R G. Quantitative assay for the measurement of immune responses directed against the human placenta. Netherlands.
Davies, M, and Sutcliffe, R G. 1982. "Quantitative assay for the measurement of immune responses directed against the human placenta." Netherlands.
@misc{etde_5135294,
title = {Quantitative assay for the measurement of immune responses directed against the human placenta}
author = {Davies, M, and Sutcliffe, R G}
abstractNote = {A quantitative in vitro immune assay based on the classical chromium release assay has been developed to detect immune responses directed against alien antigens expressed by the developing foetus and present on the maternal-facing surface of the human placenta. A plasma membrane fraction from the surface of the placenta was prepared and the vesicles thus formed were radiolabelled with /sup 51/Cr. The /sup 51/Cr-labelled vesicles, by various criteria, were found to be suitable for use as targets in a release assay. Further, by means of experimentally immunised animals, the target membranes were shown to be capable of detecting both cellular and humoral anti-placental activity.}
journal = {J. Immunol. Methods; (Netherlands)}
volume = {48:3}
journal type = {AC}
place = {Netherlands}
year = {1982}
month = {Feb}
}