Abstract
A method is described that allows the cytologic detection of the transcriptional activity of endogenous RNA polymerase in fixed Chironomus polytene chromosomes. /sup 3/H-UTP is preferentially incorporated onto nucleoli and Balbiani rings where transcription is specially high. The former incorporation was sensitive to actinomycin D while incorporation on Balbiani rings was sensitive to ..cap alpha..-amanitin. The label pattern and frequency resemble those detected by /sup 3/H-uridine incorporation in the living state.
Diez, J L;
de la Torre, C
[1]
- Consejo Superior de Investigacionis Cientificas, Madrid (Spain). Inst. Biologia Celular
Citation Formats
Diez, J L, and de la Torre, C.
/sup 3/H-UTP incorporation in polytene chromosomes of Chironomus permeabilized cells.
Austria: N. p.,
1979.
Web.
doi:10.1007/BF01293437.
Diez, J L, & de la Torre, C.
/sup 3/H-UTP incorporation in polytene chromosomes of Chironomus permeabilized cells.
Austria.
https://doi.org/10.1007/BF01293437
Diez, J L, and de la Torre, C.
1979.
"/sup 3/H-UTP incorporation in polytene chromosomes of Chironomus permeabilized cells."
Austria.
https://doi.org/10.1007/BF01293437.
@misc{etde_5130852,
title = {/sup 3/H-UTP incorporation in polytene chromosomes of Chironomus permeabilized cells}
author = {Diez, J L, and de la Torre, C}
abstractNote = {A method is described that allows the cytologic detection of the transcriptional activity of endogenous RNA polymerase in fixed Chironomus polytene chromosomes. /sup 3/H-UTP is preferentially incorporated onto nucleoli and Balbiani rings where transcription is specially high. The former incorporation was sensitive to actinomycin D while incorporation on Balbiani rings was sensitive to ..cap alpha..-amanitin. The label pattern and frequency resemble those detected by /sup 3/H-uridine incorporation in the living state.}
doi = {10.1007/BF01293437}
journal = []
volume = {101:1-2}
journal type = {AC}
place = {Austria}
year = {1979}
month = {Jan}
}
title = {/sup 3/H-UTP incorporation in polytene chromosomes of Chironomus permeabilized cells}
author = {Diez, J L, and de la Torre, C}
abstractNote = {A method is described that allows the cytologic detection of the transcriptional activity of endogenous RNA polymerase in fixed Chironomus polytene chromosomes. /sup 3/H-UTP is preferentially incorporated onto nucleoli and Balbiani rings where transcription is specially high. The former incorporation was sensitive to actinomycin D while incorporation on Balbiani rings was sensitive to ..cap alpha..-amanitin. The label pattern and frequency resemble those detected by /sup 3/H-uridine incorporation in the living state.}
doi = {10.1007/BF01293437}
journal = []
volume = {101:1-2}
journal type = {AC}
place = {Austria}
year = {1979}
month = {Jan}
}