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/sup 3/H-UTP incorporation in polytene chromosomes of Chironomus permeabilized cells

Abstract

A method is described that allows the cytologic detection of the transcriptional activity of endogenous RNA polymerase in fixed Chironomus polytene chromosomes. /sup 3/H-UTP is preferentially incorporated onto nucleoli and Balbiani rings where transcription is specially high. The former incorporation was sensitive to actinomycin D while incorporation on Balbiani rings was sensitive to ..cap alpha..-amanitin. The label pattern and frequency resemble those detected by /sup 3/H-uridine incorporation in the living state.
Authors:
Diez, J L; de la Torre, C [1] 
  1. Consejo Superior de Investigacionis Cientificas, Madrid (Spain). Inst. Biologia Celular
Publication Date:
Jan 01, 1979
Product Type:
Journal Article
Reference Number:
AIX-11-552210; EDB-80-112916
Resource Relation:
Journal Name: Protoplasma; (Austria); Journal Volume: 101:1-2
Subject:
59 BASIC BIOLOGICAL SCIENCES; CHROMOSOMES; AUTORADIOGRAPHY; POLYMERASES; TRANSCRIPTION; FLIES; RNA; TRITIUM; UPTAKE; UTP; ANIMALS; ARTHROPODS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; ENZYMES; HYDROGEN ISOTOPES; INSECTS; INVERTEBRATES; ISOTOPES; LIGHT NUCLEI; NUCLEI; NUCLEIC ACIDS; NUCLEOTIDES; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; RADIOISOTOPES; YEARS LIVING RADIOISOTOPES; 550301* - Cytology- Tracer Techniques
OSTI ID:
5130852
Country of Origin:
Austria
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: PROTA
Submitting Site:
INIS
Size:
Pages: 81-87
Announcement Date:

Citation Formats

Diez, J L, and de la Torre, C. /sup 3/H-UTP incorporation in polytene chromosomes of Chironomus permeabilized cells. Austria: N. p., 1979. Web. doi:10.1007/BF01293437.
Diez, J L, & de la Torre, C. /sup 3/H-UTP incorporation in polytene chromosomes of Chironomus permeabilized cells. Austria. doi:10.1007/BF01293437.
Diez, J L, and de la Torre, C. 1979. "/sup 3/H-UTP incorporation in polytene chromosomes of Chironomus permeabilized cells." Austria. doi:10.1007/BF01293437. https://www.osti.gov/servlets/purl/10.1007/BF01293437.
@misc{etde_5130852,
title = {/sup 3/H-UTP incorporation in polytene chromosomes of Chironomus permeabilized cells}
author = {Diez, J L, and de la Torre, C}
abstractNote = {A method is described that allows the cytologic detection of the transcriptional activity of endogenous RNA polymerase in fixed Chironomus polytene chromosomes. /sup 3/H-UTP is preferentially incorporated onto nucleoli and Balbiani rings where transcription is specially high. The former incorporation was sensitive to actinomycin D while incorporation on Balbiani rings was sensitive to ..cap alpha..-amanitin. The label pattern and frequency resemble those detected by /sup 3/H-uridine incorporation in the living state.}
doi = {10.1007/BF01293437}
journal = {Protoplasma; (Austria)}
volume = {101:1-2}
journal type = {AC}
place = {Austria}
year = {1979}
month = {Jan}
}